AU2002210282A1 - Method for extracting intact micro-organisms from sludge or biofilms - Google Patents

Method for extracting intact micro-organisms from sludge or biofilms

Info

Publication number
AU2002210282A1
AU2002210282A1 AU2002210282A AU1028202A AU2002210282A1 AU 2002210282 A1 AU2002210282 A1 AU 2002210282A1 AU 2002210282 A AU2002210282 A AU 2002210282A AU 1028202 A AU1028202 A AU 1028202A AU 2002210282 A1 AU2002210282 A1 AU 2002210282A1
Authority
AU
Australia
Prior art keywords
sludge
biofilms
sonication
organisms
extracting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002210282A
Inventor
Benaissa El Moualij
Ernst Heinen
Laurence Melen
Daniele Zorzi
Willy Zorzi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite de Liege ULG
Original Assignee
Universite de Liege ULG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite de Liege ULG filed Critical Universite de Liege ULG
Publication of AU2002210282A1 publication Critical patent/AU2002210282A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Abstract

The invention concerns a method for analysing and extracting consortium of microelements, more particularly from the microbial content of sludge or biofilms, whereby a sludge or biofilm sample, optionally diluted, is subjected to the joint, simultaneous or sequential, action of ultrasonic vibrations and an enzymatic digestion. The enzymatic digestion is carried out on a sonication supernatant using a cocktail of hydrolase enzymes. The sonication is advantageously a fractionated sonication. The sludge may be derived from a waste water purification plant.
AU2002210282A 2000-10-11 2001-10-11 Method for extracting intact micro-organisms from sludge or biofilms Abandoned AU2002210282A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BE2000/0648 2000-10-11
BE200000648 2000-10-11
PCT/BE2001/000179 WO2002031184A2 (en) 2000-10-11 2001-10-11 Method for extracting intact micro-organisms from sludge or biofilms

Publications (1)

Publication Number Publication Date
AU2002210282A1 true AU2002210282A1 (en) 2002-04-22

Family

ID=3896709

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002210282A Abandoned AU2002210282A1 (en) 2000-10-11 2001-10-11 Method for extracting intact micro-organisms from sludge or biofilms

Country Status (5)

Country Link
EP (1) EP1373553B1 (en)
AT (1) ATE364713T1 (en)
AU (1) AU2002210282A1 (en)
DE (1) DE60128962D1 (en)
WO (1) WO2002031184A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8076117B2 (en) 2004-03-18 2011-12-13 Mayo Foundation For Medical Education And Research Microbial biofilm removal methods and systems
EP2680866A1 (en) 2011-03-01 2014-01-08 Quorum Innovations, LLC Materials and methods for treating conditions associated with pathogenic biofilm
CN105273040A (en) * 2015-11-24 2016-01-27 北京工业大学 Method for extracting 0092 type filamentous bacterium high-expansion sludge close integration type extracellular polymeric substances
CN105348368A (en) * 2015-11-24 2016-02-24 北京工业大学 Extraction method for tightly-bound extracellular polymeric substance (TB-EPS) in type 0092 filamentous bacterium low-expansion sludge
CN105293849A (en) * 2015-11-24 2016-02-03 北京工业大学 Extraction method for tightly-bound extracellular polymeric substance (TB-EPS) in biological denitrification sludge
GB201617713D0 (en) 2016-10-19 2016-11-30 Q-Linea Ab Method for recovering microbial cells
CN112553290B (en) * 2020-12-11 2024-01-05 东南大学 Method for analyzing microbial activity in microbial cement-based material

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0773719B2 (en) * 1987-10-13 1995-08-09 建設省土木研究所長 Sludge treatment method
DE4421446A1 (en) * 1994-06-22 1996-01-04 Hielscher Gmbh Method for exposing sludge to microwaves within aqueous medium
WO2001093875A1 (en) * 2000-06-06 2001-12-13 Pharmacal Biotechnologies, Llc Compositions for treating biofilm

Also Published As

Publication number Publication date
WO2002031184A2 (en) 2002-04-18
EP1373553B1 (en) 2007-06-13
WO2002031184A3 (en) 2003-01-03
ATE364713T1 (en) 2007-07-15
DE60128962D1 (en) 2007-07-26
EP1373553A2 (en) 2004-01-02

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