AU2001296850A1 - Split-ubiquitin based reporter systems and methods of their use - Google Patents

Split-ubiquitin based reporter systems and methods of their use

Info

Publication number
AU2001296850A1
AU2001296850A1 AU2001296850A AU9685001A AU2001296850A1 AU 2001296850 A1 AU2001296850 A1 AU 2001296850A1 AU 2001296850 A AU2001296850 A AU 2001296850A AU 9685001 A AU9685001 A AU 9685001A AU 2001296850 A1 AU2001296850 A1 AU 2001296850A1
Authority
AU
Australia
Prior art keywords
split
methods
reporter systems
based reporter
ubiquitin based
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001296850A
Inventor
Nils Johnsson
Norbert Lehming
Alexander Varshavsky
Sandra Wittke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Agennix AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agennix AG filed Critical Agennix AG
Publication of AU2001296850A1 publication Critical patent/AU2001296850A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells
AU2001296850A 2000-08-04 2001-08-06 Split-ubiquitin based reporter systems and methods of their use Abandoned AU2001296850A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22341100P 2000-08-04 2000-08-04
US60/223,411 2000-08-04
PCT/US2001/041621 WO2002012902A2 (en) 2000-08-04 2001-08-06 Split-ubiquitin based reporter systems and methods of their use

Publications (1)

Publication Number Publication Date
AU2001296850A1 true AU2001296850A1 (en) 2002-02-18

Family

ID=22836370

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001296850A Abandoned AU2001296850A1 (en) 2000-08-04 2001-08-06 Split-ubiquitin based reporter systems and methods of their use

Country Status (5)

Country Link
US (1) US20040170970A1 (en)
EP (1) EP1315974A2 (en)
AU (1) AU2001296850A1 (en)
CA (1) CA2417888A1 (en)
WO (1) WO2002012902A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030165873A1 (en) * 2001-03-02 2003-09-04 Come Jon H. Three hybrid assay system
CA2704226A1 (en) * 2007-11-01 2009-05-07 The Arizona Board Of Regents On Behalf Of The University Of Arizona Cell-free methods for detecting protein-ligand interctions
CN104540949B (en) * 2012-08-07 2018-05-29 阿迈瑞斯公司 For stablizing the method for acetyl-coacetylase derivative compound generation
EP2772548A1 (en) 2013-02-27 2014-09-03 Universität Ulm A fluorescent reporter for determining molecular interactions
MX2021003310A (en) * 2018-09-20 2021-07-15 Washington University St Louis Engineered microorganisms and methods of making and using same.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585245A (en) * 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
US5503977A (en) * 1994-04-22 1996-04-02 California Institute Of Technology Split ubiquitin protein sensor
JP3659261B2 (en) * 1994-10-20 2005-06-15 モルフォシス・アクチェンゲゼルシャフト Targeted heterojunction of a recombinant protein to a multifunctional complex
US5610015A (en) * 1995-03-23 1997-03-11 Wisconsin Alumni Research Foundation System to detect protein-RNA interactions

Also Published As

Publication number Publication date
WO2002012902A2 (en) 2002-02-14
CA2417888A1 (en) 2002-02-14
US20040170970A1 (en) 2004-09-02
EP1315974A2 (en) 2003-06-04
WO2002012902A3 (en) 2003-03-27

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