AU2001278139A1 - Enhanced replication of hcv rna - Google Patents
Enhanced replication of hcv rnaInfo
- Publication number
- AU2001278139A1 AU2001278139A1 AU2001278139A AU7813901A AU2001278139A1 AU 2001278139 A1 AU2001278139 A1 AU 2001278139A1 AU 2001278139 A AU2001278139 A AU 2001278139A AU 7813901 A AU7813901 A AU 7813901A AU 2001278139 A1 AU2001278139 A1 AU 2001278139A1
- Authority
- AU
- Australia
- Prior art keywords
- hcv rna
- provides methods
- enhanced replication
- cells
- sub
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
Abstract
This invention provides methods of generating cells that stably replicate sub-genomic virus replicons. This invention also provides methods of generating cells that have disabled PKR activity and that stably replicate HCV sub-genomic replicons. The invention also provides methods of using the cells of the invention to screen for compounds that modulate viral RNA replication, including HCV RNA replication.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22324400P | 2000-08-04 | 2000-08-04 | |
US60/223,244 | 2000-08-04 | ||
PCT/US2001/024276 WO2002012477A2 (en) | 2000-08-04 | 2001-08-03 | Enhanced replication of hcv rna |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001278139A1 true AU2001278139A1 (en) | 2002-02-18 |
Family
ID=22835673
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001278139A Abandoned AU2001278139A1 (en) | 2000-08-04 | 2001-08-03 | Enhanced replication of hcv rna |
Country Status (7)
Country | Link |
---|---|
US (2) | US6660471B2 (en) |
EP (1) | EP1320583B1 (en) |
AT (1) | ATE318893T1 (en) |
AU (1) | AU2001278139A1 (en) |
CA (1) | CA2416633C (en) |
DE (1) | DE60117561T2 (en) |
WO (1) | WO2002012477A2 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4299540B2 (en) * | 2001-01-23 | 2009-07-22 | イステイチユート・デイ・リチエルケ・デイ・ビオロジア・モレコラーレ・ピ・アンジエレツテイ・エツセ・ピー・アー | Hepatitis C virus replicon and replicon enhanced cells |
WO2003085084A2 (en) * | 2002-04-03 | 2003-10-16 | Smithkline Beecham Corporation | Hepatitis c virus sub-genomic replicons |
EP2689787A1 (en) | 2010-09-03 | 2014-01-29 | Stem Centrx, Inc. | Identification and enrichment of cell subpopulations |
US9778264B2 (en) | 2010-09-03 | 2017-10-03 | Abbvie Stemcentrx Llc | Identification and enrichment of cell subpopulations |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5840565A (en) * | 1995-08-22 | 1998-11-24 | The Regents Of The University Of California | Methods for enhancing the production of viral vaccines in PKR-deficient cell culture |
US6740519B1 (en) * | 1997-11-03 | 2004-05-25 | Mount Sinai School Of Medicine | Immortalized, homozygous stat1-deficient mammalian cell lines and their uses |
-
2001
- 2001-08-03 AU AU2001278139A patent/AU2001278139A1/en not_active Abandoned
- 2001-08-03 DE DE60117561T patent/DE60117561T2/en not_active Expired - Lifetime
- 2001-08-03 CA CA2416633A patent/CA2416633C/en not_active Expired - Fee Related
- 2001-08-03 AT AT01956106T patent/ATE318893T1/en active
- 2001-08-03 EP EP01956106A patent/EP1320583B1/en not_active Expired - Lifetime
- 2001-08-03 US US09/922,962 patent/US6660471B2/en not_active Expired - Lifetime
- 2001-08-03 WO PCT/US2001/024276 patent/WO2002012477A2/en active IP Right Grant
-
2003
- 2003-10-14 US US10/684,846 patent/US7183107B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE60117561D1 (en) | 2006-04-27 |
EP1320583B1 (en) | 2006-03-01 |
US7183107B2 (en) | 2007-02-27 |
WO2002012477A2 (en) | 2002-02-14 |
CA2416633C (en) | 2013-04-30 |
US20040076612A1 (en) | 2004-04-22 |
DE60117561T2 (en) | 2007-01-11 |
WO2002012477A3 (en) | 2003-04-10 |
US20020142455A1 (en) | 2002-10-03 |
CA2416633A1 (en) | 2002-02-14 |
US6660471B2 (en) | 2003-12-09 |
EP1320583A2 (en) | 2003-06-25 |
ATE318893T1 (en) | 2006-03-15 |
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