AU2001274580A1 - Cds1 gene-knockout cells and mouse and utilization thereof - Google Patents

Cds1 gene-knockout cells and mouse and utilization thereof

Info

Publication number
AU2001274580A1
AU2001274580A1 AU2001274580A AU7458001A AU2001274580A1 AU 2001274580 A1 AU2001274580 A1 AU 2001274580A1 AU 2001274580 A AU2001274580 A AU 2001274580A AU 7458001 A AU7458001 A AU 7458001A AU 2001274580 A1 AU2001274580 A1 AU 2001274580A1
Authority
AU
Australia
Prior art keywords
cds1
alleles
inactivated
cell lines
mice
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001274580A
Inventor
Kyoji Ikeda
Noboru Motoyama
Hiroyuki Takai
Miho Watanabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chugai Pharmaceutical Co Ltd
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of AU2001274580A1 publication Critical patent/AU2001274580A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Animal Husbandry (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present inventors established ES cell lines in which one of Cds1 (Chk2) alleles was inactivated by using gene targeting. Chimeric mice were constructed using the cell lines, and genetically modified mice in which both of the Cds1 (Chk2) alleles were inactivated were constructed. Furthermore, by incubating the ES cell lines in which one of Cds1 (Chk2) alleles was inactivated in a selection medium containing a high concentration of an antibiotic, the present inventors constructed mouse ES cell lines in which both the alleles were inactivated. The mice and cells established from the mice can be utilized in screenings for compounds that regulate the cell cycle.
AU2001274580A 2000-06-21 2001-06-21 Cds1 gene-knockout cells and mouse and utilization thereof Abandoned AU2001274580A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2000191095 2000-06-21
JP2000-191095 2000-06-21
JP2000-378070 2000-12-12
JP2000378070 2000-12-12
PCT/JP2001/005314 WO2001098465A1 (en) 2000-06-21 2001-06-21 Cds1 GENE-KNOCKOUT CELLS AND MOUSE AND UTILIZATION THEREOF

Publications (1)

Publication Number Publication Date
AU2001274580A1 true AU2001274580A1 (en) 2002-01-02

Family

ID=26594671

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001274580A Abandoned AU2001274580A1 (en) 2000-06-21 2001-06-21 Cds1 gene-knockout cells and mouse and utilization thereof

Country Status (6)

Country Link
US (1) US20030226158A1 (en)
EP (1) EP1304371B1 (en)
AT (1) ATE350468T1 (en)
AU (1) AU2001274580A1 (en)
DE (1) DE60125773D1 (en)
WO (1) WO2001098465A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2292812T3 (en) 2001-10-19 2008-03-16 Ortho-Mcneil Pharmaceutical, Inc. 2-FENIL BENZIMIDAZOL AND IMIDAZO- (4,5) -PIRIDINAS AS INHIBITORS OF CDS1 / CHK2 AND COADYUVANTS FOR CHEMOTHERAPY OR RADIOTHERAPY IN THE TREATMENT OF CANCER.
US7132440B2 (en) 2003-04-17 2006-11-07 Janssen Pharmaceutica, N.V. Substituted benzimidazoles and imidazo-[4,5]-pyridines
EP1824832B1 (en) 2004-06-30 2011-04-27 Janssen Pharmaceutica NV Aryl-substituted benzimidazole and imidazopyridine ethers as anti-cancer agents
CN107058233A (en) * 2017-05-03 2017-08-18 上海长海医院 A kind of method for reducing tumour cell to the drug resistance of antineoplastic

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors

Also Published As

Publication number Publication date
WO2001098465A1 (en) 2001-12-27
US20030226158A1 (en) 2003-12-04
DE60125773D1 (en) 2007-02-15
EP1304371A1 (en) 2003-04-23
ATE350468T1 (en) 2007-01-15
EP1304371B1 (en) 2007-01-03
EP1304371A4 (en) 2004-11-10

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