AU2001252438A1 - A modified expandase and uses thereof - Google Patents

A modified expandase and uses thereof

Info

Publication number
AU2001252438A1
AU2001252438A1 AU2001252438A AU5243801A AU2001252438A1 AU 2001252438 A1 AU2001252438 A1 AU 2001252438A1 AU 2001252438 A AU2001252438 A AU 2001252438A AU 5243801 A AU5243801 A AU 5243801A AU 2001252438 A1 AU2001252438 A1 AU 2001252438A1
Authority
AU
Australia
Prior art keywords
modified expandase
expandase
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001252438A
Inventor
Robert Ian Johnson
Roger William Newbert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ACS Dobfar UK Ltd
Original Assignee
ACS Dobfar UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ACS Dobfar UK Ltd filed Critical ACS Dobfar UK Ltd
Publication of AU2001252438A1 publication Critical patent/AU2001252438A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/20—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and the other dehydrogenated (1.14.20)
    • C12Y114/20001—Deacetoxycephalosporin-C synthase (1.14.20.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2001252438A 2000-05-09 2001-05-09 A modified expandase and uses thereof Abandoned AU2001252438A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0011185 2000-05-09
GBGB0011185.6A GB0011185D0 (en) 2000-05-09 2000-05-09 Protein
PCT/GB2001/002047 WO2001085951A1 (en) 2000-05-09 2001-05-09 A modified expandase and uses thereof

Publications (1)

Publication Number Publication Date
AU2001252438A1 true AU2001252438A1 (en) 2001-11-20

Family

ID=9891262

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001252438A Abandoned AU2001252438A1 (en) 2000-05-09 2001-05-09 A modified expandase and uses thereof

Country Status (3)

Country Link
AU (1) AU2001252438A1 (en)
GB (1) GB0011185D0 (en)
WO (1) WO2001085951A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6699699B2 (en) * 2002-03-26 2004-03-02 Synmax Biochemical Co., Ltd. Mutated penicillin expandases
GB0319579D0 (en) * 2003-08-20 2003-09-24 Isis Innovation Enzyme
WO2005103261A1 (en) * 2004-04-22 2005-11-03 Orchid Chemicals & Pharmaceuticals Ltd. Modified expandase enzyme and its use
BRPI0515976A (en) * 2004-10-13 2008-08-12 Dsm Ip Assets Bv mutant expandases
EP1902140A2 (en) * 2005-07-12 2008-03-26 DSMIP Assets B.V. Mutant expandases and their use in the production of beta-lactam compounds
WO2007023369A1 (en) * 2005-08-25 2007-03-01 Orchid Chemicals & Pharmaceuticals Limited Modified expandase-hydroxylase and its applications
WO2008107782A2 (en) * 2007-03-06 2008-09-12 Orchid Chemicals & Pharmaceuticals Limited Modified hydroxylase and its applications
US9404139B2 (en) 2012-01-10 2016-08-02 Orchid Chemicals & Pharmaceuticals Limited Mutated cephalosporin hydroxylase and its application in deacetylcephalosporanic acid synthesis
CN107557348B (en) * 2016-07-01 2022-07-08 百瑞全球有限公司 Cephalosporin hydroxylase mutants, DNA encoding the same, methods of using the same and uses thereof
CN112852912B (en) * 2020-04-10 2022-01-11 中国科学院天津工业生物技术研究所 Method for synthesizing 7-aminodesacetoxycephalosporanic acid
CN114958877B (en) * 2022-06-14 2024-02-20 河南省健康元生物医药研究院有限公司 Deacetylated oxo cephalosporin C synthetase mutant, encoding gene and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020053A2 (en) * 1995-11-27 1997-06-05 Gist-Brocades B.V. Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g
EP1047785A1 (en) * 1997-12-24 2000-11-02 Isis Innovation Limited Modified deacetoxycephalosporin c synthase (daocs) and x-ray structure
ID27054A (en) * 1998-05-19 2001-02-22 Dsm Nv IMPROVING THE PRODUCTION OF SEPALOSPORIN IN LIVING ORGANISMS

Also Published As

Publication number Publication date
GB0011185D0 (en) 2000-06-28
WO2001085951A1 (en) 2001-11-15

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