AU2001244140A1 - Method for removing endotoxins - Google Patents

Method for removing endotoxins

Info

Publication number
AU2001244140A1
AU2001244140A1 AU2001244140A AU4414001A AU2001244140A1 AU 2001244140 A1 AU2001244140 A1 AU 2001244140A1 AU 2001244140 A AU2001244140 A AU 2001244140A AU 4414001 A AU4414001 A AU 4414001A AU 2001244140 A1 AU2001244140 A1 AU 2001244140A1
Authority
AU
Australia
Prior art keywords
removing endotoxins
endotoxins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001244140A
Inventor
Robertus Hendriks
Andrea Lantos
Maria Wehsling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of AU2001244140A1 publication Critical patent/AU2001244140A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AU2001244140A 2000-03-06 2001-02-14 Method for removing endotoxins Abandoned AU2001244140A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10010342 2000-03-06
DE10010342A DE10010342A1 (en) 2000-03-06 2000-03-06 Method for reducing the endotoxin content of nucleic acid (I) is derived from natural, genetic engineering or biotechnological sources is used to produce high-purity plasmid DNA from natural sources
PCT/EP2001/001610 WO2001066718A1 (en) 2000-03-06 2001-02-14 Method for removing endotoxins

Publications (1)

Publication Number Publication Date
AU2001244140A1 true AU2001244140A1 (en) 2001-09-17

Family

ID=7633346

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001244140A Abandoned AU2001244140A1 (en) 2000-03-06 2001-02-14 Method for removing endotoxins

Country Status (6)

Country Link
US (1) US6617443B2 (en)
EP (1) EP1261702B1 (en)
JP (1) JP2003525630A (en)
AU (1) AU2001244140A1 (en)
DE (2) DE10010342A1 (en)
WO (1) WO2001066718A1 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030192053A1 (en) * 1997-02-19 2003-10-09 Next Level Communications, Inc. Method and apparatus for transmitting wireless signals over media
US20040083493A1 (en) * 1997-02-19 2004-04-29 Next Level Communications, Inc. Transmitting caller ID within a digital stream
CN1643145A (en) * 2002-03-21 2005-07-20 艾夫西亚生物科技公司 Purification methods for oligonucleotides and their analogs
DE10393326D2 (en) 2002-06-24 2005-06-02 Profos Ag Method for detection and removal of endotoxin
AU2003267175A1 (en) 2002-09-13 2004-04-30 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
FR2848223B1 (en) * 2002-12-09 2005-02-25 Centre Nat Rech Scient NOVEL METHOD OF ISOLATING ENDOTOXINS.
US20060189790A1 (en) * 2003-06-24 2006-08-24 Akzo Nobel N.V. Removal of lipopolysaccharides from protein-lipopolysaccharide complexes by non flammable solvents.
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
DE102005002969A1 (en) 2005-01-21 2006-08-03 Profos Ag Method for detection and removal of endotoxin
JP2010520320A (en) 2007-02-28 2010-06-10 リポクセン テクノロジーズ リミテッド Reduction of endotoxin in polysialic acid
JP5693449B2 (en) * 2008-04-30 2015-04-01 グラダリス インク.Gradalis, Inc. High-purity plasmid DNA preparation and preparation method thereof
US20160215010A1 (en) * 2013-10-03 2016-07-28 Kyowa Hakko Bio Co., Ltd. Method for purifying double-stranded ribonucleic acid
CN108148831A (en) * 2018-01-15 2018-06-12 南京驯鹿医疗技术有限公司 A kind of a large amount of preparation processes of endotoxin-free plasmid
EP3761956B1 (en) * 2018-03-08 2024-06-12 Catalent U.K. Swindon Zydis Limited Process to reduce endotoxin in gelatin
EP4112729B1 (en) 2021-07-02 2023-09-06 AXAGARIUS GmbH & Co. KG Mixture of branched secondary alcohol ethoxylates for removal of endotoxins in anion exchange chromatography
CN117751186A (en) * 2021-08-05 2024-03-22 默克专利股份公司 Methods for reducing endotoxin levels for nucleic acid purification
WO2023170028A1 (en) 2022-03-10 2023-09-14 Merck Patent Gmbh Method for reducing endotoxin levels in nucleic acid purification

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021179A1 (en) * 1994-02-07 1995-08-10 Qiagen Gmbh Endotoxin reduction or removal process
AU2127395A (en) * 1994-03-22 1995-10-09 Rhone-Poulenc Rorer Pharmaceuticals Inc. Highly efficient production and isolation of viral particles
US6825012B2 (en) * 1995-02-23 2004-11-30 Gencell S.A. DNA molecules, preparation and use in gene therapy
US5837520A (en) 1995-03-07 1998-11-17 Canji, Inc. Method of purification of viral vectors
WO1999063076A1 (en) * 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification

Also Published As

Publication number Publication date
DE50113292D1 (en) 2008-01-03
US20030068810A1 (en) 2003-04-10
US6617443B2 (en) 2003-09-09
EP1261702B1 (en) 2007-11-21
EP1261702A1 (en) 2002-12-04
JP2003525630A (en) 2003-09-02
DE10010342A1 (en) 2001-09-20
WO2001066718A1 (en) 2001-09-13

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