AU2001238106A1 - Method for amplifying full length single strand polynucleotide sequences - Google Patents

Method for amplifying full length single strand polynucleotide sequences

Info

Publication number
AU2001238106A1
AU2001238106A1 AU2001238106A AU3810601A AU2001238106A1 AU 2001238106 A1 AU2001238106 A1 AU 2001238106A1 AU 2001238106 A AU2001238106 A AU 2001238106A AU 3810601 A AU3810601 A AU 3810601A AU 2001238106 A1 AU2001238106 A1 AU 2001238106A1
Authority
AU
Australia
Prior art keywords
full length
polynucleotide sequences
single strand
strand polynucleotide
length single
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001238106A
Inventor
James R. Connor
Zheng Ye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Penn State Research Foundation
Original Assignee
Penn State Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Penn State Research Foundation filed Critical Penn State Research Foundation
Publication of AU2001238106A1 publication Critical patent/AU2001238106A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
AU2001238106A 2000-02-10 2001-02-09 Method for amplifying full length single strand polynucleotide sequences Abandoned AU2001238106A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US18161500P 2000-02-10 2000-02-10
US60/181,615 2000-02-10
US20303500P 2000-05-09 2000-05-09
US60/203,035 2000-05-09
PCT/US2001/004259 WO2001059101A1 (en) 2000-02-10 2001-02-09 Method for amplifying full length single strand polynucleotide sequences

Publications (1)

Publication Number Publication Date
AU2001238106A1 true AU2001238106A1 (en) 2001-08-20

Family

ID=26877341

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001238106A Abandoned AU2001238106A1 (en) 2000-02-10 2001-02-09 Method for amplifying full length single strand polynucleotide sequences

Country Status (3)

Country Link
US (1) US20020004592A1 (en)
AU (1) AU2001238106A1 (en)
WO (1) WO2001059101A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003294447A1 (en) * 2002-11-21 2004-06-18 Epicentre Technologies Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
WO2004070053A2 (en) * 2003-02-03 2004-08-19 Amersham Biosciences Corporation cDNA AMPLIFICATION FOR EXPRESSION PROFILING
WO2005010159A2 (en) * 2003-07-17 2005-02-03 Children's Hospital Medical Center Rolling circle amplification of micro-rna samples
WO2008143774A2 (en) * 2007-05-01 2008-11-27 University Of Massachusetts Methods and compositions for locating snp heterozygosity for allele specific diagnosis and therapy
US20130304180A1 (en) * 2012-05-09 2013-11-14 Michael L. Green Catheter having dual balloon hydraulic actuator
CN103468670B (en) * 2012-06-08 2016-01-20 上海欧孚生物医药科技有限公司 Full-length cDNA nucleic acid linear amplification method and test kit
US10655170B2 (en) 2016-07-06 2020-05-19 Takara Bio Usa, Inc. Coupling adaptors to a target nucleic acid
CN111635930B (en) * 2020-05-12 2023-10-24 中国农业科学院农业基因组研究所 Method for extracting unknown RNA full-length sequence by high-throughput sequencing

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994370A (en) * 1989-01-03 1991-02-19 The United States Of America As Represented By The Department Of Health And Human Services DNA amplification technique
EP0542874A4 (en) * 1990-07-25 1994-05-11 Syngene Inc Circular extension for generating multiple nucleic acid complements
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
CA2220530A1 (en) * 1995-06-02 1996-12-05 Incyte Pharmaceuticals, Inc. Improved method for obtaining full-length cdna sequences
WO1997019193A2 (en) * 1995-11-21 1997-05-29 Yale University Unimolecular segment amplification and detection

Also Published As

Publication number Publication date
WO2001059101A1 (en) 2001-08-16
WO2001059101A9 (en) 2002-10-17
US20020004592A1 (en) 2002-01-10

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