AU2001235580A1 - Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses - Google Patents

Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses

Info

Publication number
AU2001235580A1
AU2001235580A1 AU2001235580A AU3558001A AU2001235580A1 AU 2001235580 A1 AU2001235580 A1 AU 2001235580A1 AU 2001235580 A AU2001235580 A AU 2001235580A AU 3558001 A AU3558001 A AU 3558001A AU 2001235580 A1 AU2001235580 A1 AU 2001235580A1
Authority
AU
Australia
Prior art keywords
protein
functional groups
reagent
particle
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001235580A
Inventor
Ahmed Arkis
Thierry Delair
Abdelhamid Elaissari
Bernard Mandrand
Doran Spencer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Publication of AU2001235580A1 publication Critical patent/AU2001235580A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Soft Magnetic Materials (AREA)

Abstract

The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
AU2001235580A 2000-01-21 2001-01-22 Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses Abandoned AU2001235580A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0000862A FR2804117B1 (en) 2000-01-21 2000-01-21 PROCESS FOR ISOLATING PROTEINS AND / OR NUCLEIC ACIDS, PARTICLE COMPLEXES AND PROTEINS AND / OR NUCLEIC ACIDS, REAGENT AND APPLICATIONS
FR0000862 2000-01-21
PCT/FR2001/000205 WO2001052612A2 (en) 2000-01-21 2001-01-22 Method for isolating proteins or protein and nucleic acid associations, and resulting complexes of particles and proteins

Publications (1)

Publication Number Publication Date
AU2001235580A1 true AU2001235580A1 (en) 2001-07-31

Family

ID=8846236

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001235580A Abandoned AU2001235580A1 (en) 2000-01-21 2001-01-22 Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses

Country Status (9)

Country Link
US (3) US7060804B2 (en)
EP (1) EP1248679B1 (en)
AT (1) ATE290433T1 (en)
AU (1) AU2001235580A1 (en)
CA (1) CA2412536A1 (en)
DE (1) DE60109272T2 (en)
ES (1) ES2237554T3 (en)
FR (1) FR2804117B1 (en)
WO (1) WO2001052612A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10256892A1 (en) * 2002-11-29 2004-06-09 Henkel Kgaa New magnetic particles for the separation of microorganisms from technical media
US20070003975A1 (en) * 2003-05-02 2007-01-04 Canon Kabushiki Kaisha Structured construct and producing method therefor
ATE405591T1 (en) * 2003-08-26 2008-09-15 Univ Danmarks Tekniske CONTINUOUS PROCESS FOR ARRANGEMENT OF MACROMOLECULAR SUBSTANCES AND THE SUBSEQUENT RECORDING AND ISOLATION OF A MACROMOLECULAR ARRANGEMENT, AND A SYSTEM SUITABLE FOR THIS PROCESS
US20060263897A1 (en) * 2003-09-09 2006-11-23 Koninklijke Philips Electronics N.V. Nanoparticles for detecting analytes
US8426126B2 (en) * 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
CN1861628B (en) * 2005-05-11 2010-05-05 上海师范大学 Process for separating protein of Chinese medicine
JP4716034B2 (en) * 2006-03-24 2011-07-06 Ｊｓｒ株式会社 Magnetic particles and method for producing the same
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US20100267933A1 (en) 2006-12-21 2010-10-21 Moya Wilson Purification of proteins
US7981688B2 (en) * 2007-03-08 2011-07-19 University Of Washington Stimuli-responsive magnetic nanoparticles and related methods
EP3533461A3 (en) 2007-03-26 2019-12-18 Academisch Ziekenhuis Leiden h.o.d.n. LUMC Prame derived peptides and immunogenic compositions comprising these
US20100018674A1 (en) * 2008-07-22 2010-01-28 Donald John Enzinna Reservoir with moveable partition for quick recovery
JP5326443B2 (en) * 2008-09-05 2013-10-30 Ｊｎｃ株式会社 Temperature-responsive magnetic fine particles that can be freeze-dried
ES2749232T3 (en) 2008-12-16 2020-03-19 Emd Millipore Corp Stirred tank reactor and procedure
US8426214B2 (en) * 2009-06-12 2013-04-23 University Of Washington System and method for magnetically concentrating and detecting biomarkers
US9080933B2 (en) 2009-11-09 2015-07-14 University Of Washington Through Its Center For Commercialization Stimuli-responsive polymer diagnostic assay comprising magnetic nanoparticles and capture conjugates
US20110117668A1 (en) * 2009-11-09 2011-05-19 University Of Washington Through Its Center For Commercialization Self-powered smart diagnostic devices
SG10201804385YA (en) 2010-05-17 2018-06-28 Emd Millipore Corp Stimulus responsive polymers for the purification of biomolecules
WO2012155097A2 (en) * 2011-05-11 2012-11-15 Luminex Corporation Systems and methods for producing an evaporation barrier in a reaction chamber
EP2971188A1 (en) * 2013-03-15 2016-01-20 The U.S.A. as represented by the Secretary, Department of Health and Human Services Selective detection of hepatitis a, b, c, d, or e viruses or combinations thereof
DE102016121483B4 (en) * 2016-11-09 2020-06-18 Axagarius Gmbh & Co. Kg Particulate solid composite material for nucleic acid purification, containing magnetic nanoparticles, process for its production and its use

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4018886A (en) * 1975-07-01 1977-04-19 General Electric Company Diagnostic method and device employing protein-coated magnetic particles
DE3517333A1 (en) * 1985-05-14 1986-11-20 Basf Ag, 6700 Ludwigshafen METHOD FOR PRODUCING STABLE DISPERSIONS OF FINE PARTICULATE POLYISOCYANATES AND THE USE THEREOF
US5051469A (en) * 1985-12-13 1991-09-24 Monsanto Company Rubber modified reaction molded nylon-6 block copolymers
EP0344270B1 (en) * 1987-10-26 1994-11-30 Baxter Diagnostics Inc. Process for producing magnetically responsive polymer particles and application thereof
US5049469A (en) * 1989-12-27 1991-09-17 Eastman Kodak Company Toner image pressure transfer method and toner useful therefor
FR2707010B1 (en) * 1993-06-25 1995-09-29 Bio Merieux
US5756273A (en) * 1996-02-06 1998-05-26 Eastman Kodak Company Photographic element containing a core/shell polymer latex
US5976382A (en) 1996-07-10 1999-11-02 Ligochem, Inc. Removal of proteins from aqueuos media by precipitation
FR2762394B1 (en) * 1997-04-16 1999-05-28 Bio Merieux LIGAND COORDINATION COMPOUND AND USE FOR FIXING BIOLOGICAL MATERIAL
WO1998051435A1 (en) * 1997-05-14 1998-11-19 Weixin Tang Fully-coated, uniform-sized metallic particles
FR2767137A1 (en) * 1997-08-07 1999-02-12 Bio Merieux COMPLEX CHEMICAL COMPOUND, SYNTHESIS AND VARIOUS APPLICATIONS OF THE SAID COMPOUND
FR2773416B1 (en) * 1998-01-06 2000-02-11 Bio Merieux IMPROVED MAGNETIC PARTICLES, PROCESSES FOR OBTAINING SAME AND THEIR USES IN THE SEPARATION OF MOLECULES
DE19803098A1 (en) 1998-01-28 1999-07-29 Basf Ag Particulate polymer, useful as impact modifier and/or dulling additive for thermoplastic molding materials
FR2800635B1 (en) * 1999-11-05 2002-07-26 Bio Merieux COMPOSITE NANOSPHERES, DERIVATIVE CONJUGATES, METHOD OF PREPARATION AND USES THEREOF
FR2800636A1 (en) * 1999-11-05 2001-05-11 Bio Merieux COMPOSITE PARTICLES, DERIVATIVE CONJUGATES, PROCESS FOR THEIR PREPARATION AND USES

Also Published As

Publication number Publication date
EP1248679A2 (en) 2002-10-16
US7060804B2 (en) 2006-06-13
WO2001052612A3 (en) 2002-03-07
WO2001052612A2 (en) 2001-07-26
FR2804117B1 (en) 2004-08-20
US20100209987A1 (en) 2010-08-19
ATE290433T1 (en) 2005-03-15
EP1248679B1 (en) 2005-03-09
US20030175691A1 (en) 2003-09-18
CA2412536A1 (en) 2001-07-26
DE60109272D1 (en) 2005-04-14
DE60109272T2 (en) 2006-04-13
ES2237554T3 (en) 2005-08-01
US8093026B2 (en) 2012-01-10
US7700744B2 (en) 2010-04-20
US20060105326A1 (en) 2006-05-18
FR2804117A1 (en) 2001-07-27

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