AU2001231749A1 - A method of preparing recombinant nucleic acids - Google Patents

A method of preparing recombinant nucleic acids

Info

Publication number
AU2001231749A1
AU2001231749A1 AU2001231749A AU3174901A AU2001231749A1 AU 2001231749 A1 AU2001231749 A1 AU 2001231749A1 AU 2001231749 A AU2001231749 A AU 2001231749A AU 3174901 A AU3174901 A AU 3174901A AU 2001231749 A1 AU2001231749 A1 AU 2001231749A1
Authority
AU
Australia
Prior art keywords
nucleic acids
recombinant nucleic
preparing recombinant
preparing
acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001231749A
Inventor
Stefan B. Hosch
Jakob R. Izbicki
Viacheslav Kalinin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU2001231749A1 publication Critical patent/AU2001231749A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001231749A 2000-02-14 2001-02-14 A method of preparing recombinant nucleic acids Abandoned AU2001231749A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00102973 2000-02-14
EP00102973A EP1130092A1 (en) 2000-02-14 2000-02-14 A method of preparing recombinant nucleic acids
PCT/EP2001/001637 WO2001059099A1 (en) 2000-02-14 2001-02-14 A method of preparing recombinant nucleic acids

Publications (1)

Publication Number Publication Date
AU2001231749A1 true AU2001231749A1 (en) 2001-08-20

Family

ID=8167845

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001231749A Abandoned AU2001231749A1 (en) 2000-02-14 2001-02-14 A method of preparing recombinant nucleic acids

Country Status (3)

Country Link
EP (1) EP1130092A1 (en)
AU (1) AU2001231749A1 (en)
WO (1) WO2001059099A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6730500B1 (en) * 2000-08-30 2004-05-04 Zymogenetics, Inc. Methods for generating a continuous nucleotide sequence from noncontiguous nucleotide sequences
EP1398375A1 (en) * 2002-09-13 2004-03-17 Cosmix Molecular Biologicals GmbH Recombination process for recombining fragments of variant polynucleotides
WO2009025209A1 (en) * 2007-08-21 2009-02-26 National University Corporation Hokkaido University Method for preparation of recombinant dna

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US6261797B1 (en) * 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
WO1998038326A1 (en) * 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof

Also Published As

Publication number Publication date
EP1130092A1 (en) 2001-09-05
WO2001059099A1 (en) 2001-08-16

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