AU1473102A - Immunoassay testing kit and containers - Google Patents

Description

S&FRef: 536359D1

AUSTRALIA

PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT

ORIGINAL

Name and Address of Applicant: Actual Inventor(s): Address for Service: Molecular Circuitry, Inc.

Suite 800, 401 City Avenue Bala Cynwyd Pennsylvania 19004 United States of America Ricky T. Smethers, William B. Greger, Wendy R.

Whitcomb Spruson Ferguson St Martins Tower,Level 31 Market Street Sydney NSW 2000 (CCN 3710000177) Immunoassay Testing Kit and Containers Invention Title: The following statement is a full description of this invention, including the best method of performing it known to me/us:- 3 1 As J v 5845c c -1 ii: IMMUNOASSAY TESTING KIT AND CONTAINERS CROSS REFERENCES TO RELATED APPLICATIONS Not Applicable STATEMENT REGARDING FEDERALLY SPONSORED

RESEARCH

Not Applicable 1 0 FIELD OF THE INVENTION The field of invention is electrical immunoassay testing, more particularly containers for simultaneous immunoassay testing.

BACKGROUND OF THE INVENTION Electrical immunoassay testing methods have been S**developeR. td,determine the presence of foreign 20 substances, microorganisms, or endogenous substances. As a result, these testing methods may be used to detect, bacteria, viruses, or substances relating to drug abuse, human hormones, nucleic acids and the like. Such methods detect a change in electrical properties, such as current, voltage, or resistance. The change in electrical properties indicate that an antigen-antibody reaction has occurred.

i "xi- -r i.n. -~irii*r.rr i-^IC;r- 2 One particular electrical immunoassay method described in U.S. Patent 5,284,748 provides a method for detecting a binding reaction between a pair of first and second substances, particularly biogenic substances, which specifically bind together causing full or partial completion of an essentially open electrical circuit.

This method is especially useful for detecting antigens in the fluids or tissues of humans or animals. Antigens are chemicals that when introduced to the body provoke production of an antibody. Antigens include drugs, eoooe toxins, hormones, allergens, tumor markers, factors, enzymes, steroids, nucleotides and other substances.

U.S. Patent 5,284,748 is fully incorporated herein by reference.

The mechanics of implementing the above method, however, have been limited to a manual single batch *process that is not commercially viable. A technician must perform the test on an individual sample using multiple reagents and other fluids from individual containers, carefully transferring the reagents to the sample container to avoid contamination and waste.

Automated immunoassay testing devices incorporating the testing method disclosed in U.S. Patent 5,284,748 have not been disclosed heretofore. The method requires multiple transfers of reagents and other fluids to a test specimen in a test cartridge. In addition, the container holding the test sample requires special circuitry that changes resistivity in the presence of the binding reaction disclosed in U.S. Patent 5,284,748.

-s OBJECT OF THE INVENTION It is an object of the present invention to overcome or ameliorate some of the disadvantages of the prior art, or at least to provide a useful alternative.

SUMMARY OF THE INVENTION Accordingly, in a first aspect, the present invention provides a testing kit for use in an immunoassay testing device that detects a binding reaction between a pair of first and second substances that specifically bind together to form electrically conductive particles which fully or partially complete an open electrical circuit, said device having a io conveying mechanism with a plurality of predetermined container positions, said kit comprising: a first container receivable by said conveying mechanism having at least three groups of cavities, each group comprising a plurality of cavities for receiving fluids, at "•oleast one of said fluids is one of said substances.

15 at least one second container receivable by said conveying mechanism to be S: displaced along the axis of motion of said conveying mechanism, said second container including at least one upwardly opening reaction cavity defined by front, rear and side walls joined by a bottom; at least one pair of electrical contacts which extend into said reaction cavity and define open ends of a circuit, wherein transferring at least one of said fluids from said first i container to said reaction cavity causes a binding reaction in the presence of said other S.substance to form electrically conductive particles which electrically connect said at least one pair of electrical contacts.

S•In a second aspect, the present invention provides a container for use in an 25 immunoassay testing device that detects a binding reaction between a pair of first and second substances that specifically bind together to form electrically conductive particles which fully or partially complete an open electrical circuit, said second container comprising: at least one upwardly opening reaction cavity defined by front, rear and side walls joined by a bottom; at least one pair of electrical contacts which extend into said reaction cavity and define open ends of a circuit, wherein transferring at least one substance to said reaction cavity causes a binding reaction in the presence of another substance to form electrically conductive particles which electrically connect said at least one pair of electrical contacts.

The kit preferably provides self contained reagent and testing packages that are less contaminant prone and subject to minimal waste. The kit has a self contained reagent package and a test cartridge that are accessible by the testing device. The reagent [R:\LIBLL] 12080.doc:caa ~1 cr 1 -4package has cavities for reagents and diluting fluids. The test cartridge has a cavity for the test specimen and circuitry that is closed in the presence of the binding reaction when an electrical current is applied to contacts at the top of the cartridge. Thus accomplishing a general objective of preferably providing a self-container test kit that minimizes waste.

The present invention preferably provides a prepackaged reagent package that can be used in the immunoassay testing device without the risk of contamination. This is accomplished by providing a container having multiple cavities formed in the top containing reagents, rinsing fluid, and for receiving aspirated fluid. The top is sealed by a penetrable foil film.

The present invention preferably provides a test cartridge that can be used in an automated immunoassay testing device. This is accomplished by mounting a resistor on a container cavity sidewall that is compatible with the immunoassay testing method.

BRIEF DESCRIPTION OF THE DRAWINGS A preferred embodiment of the invention will now be described, by way of example only, S:I" 15 with reference to the accompanying drawings.

*Figure 1 is a perspective view of an automated immunoassay testing device which employs the present invention; Fi Figure 2 is a perspective view of the automated immunoassay testing device of Fig. 1 with the housing removed; Figure 3 is a top plan view of embodiments of the present invention on a S.carousel in the automated immunoassay testing device of Fig. 1; Figure 4 is a top perspective view of a first embodiment of the invention used in the automated immunoassay testing device of Fig. 1; SFigure 5 is a bottom perspective view of the embodiment of Fig. 4; Figure 6 is a top plan view of the embodiment of Fig. 4; Figure 7 is a top perspective view of a second embodiment of the invention used in the automated immunoassay testing device of Fig. 1; [R:\LIBLL] 1 2080.doc:caa 7 77,,- Figure 8 is a top plan view of the embodiment of Fig. 7; Figure 9 is a side plan view of the embodiment of Fig. 7; and Figure 10 is a perspective view of a resistor sheet used in the embodiment of Fig. 7.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Referring particularly to Figs. 1 and 2, an ":'":immunoassay testing device 10 that detects a binding reaction between a pair of first and second substances, particularly biogenic substances, which specifically bind together causing full or partial completion of an essentially open electrical circuit employs embodiments of the present invention. The testing device 10 is **mounted on a base 1 and includes an aluminum housing 2 that supports a keyboard 4, a floppy disk drive 5, and a display 6 on its front surface. A pivotally mounted cover 8 provides access to a circular carousel 12, disposed within the housing 2. The carousel 12 is a conveying mechanism that indexes the containers 14 to various positions within the testing device As shown in Fig. 3, containers 14 such as reagent packages 16 containing fluid reagents used in the testing process and test cartridges 18 for use with testing specimens, forming a kit are placed on the carousel 12. The carousel 12 has indents 42 on an inner wall 15 and outer wall 17 that receive corresponding tabs on the containers 14. The indents 42 define i 6 predetermined container locations on the carousel 12. The containers 14 are disposed between the carousel walls and 17. Preferably, up to 9 reagents packages 16 and 27 test cartridges 18 can be placed on the carousel for simultaneous automated testing. Preferably, each reagent package 16 can provide reagents for up to three adjacent test cartridges 18.

Following placement of each container 14 on the carousel 12, the rotating carousel 12 conveys one of the containers 16 to a position disposed beneath a fluidics mechanism 20 to initiate the immunoassay testing procedure. The carousel index position is determined by a first optical sensing unit 21 that detects indents 23 formed on the carousel periphery. The containers 14 are indexed by the carousel 12 in cooperation with a second optical sensing unit 22 adjacent to the carousel that senses a locating mechanism on a container 14.

As shown in Fig. 2, the fluidics mechanism 20 is o arranged to cooperate with the reagent package 16 and test cartridge 18 as the containers 14 are indexed on the carousel 12. The fluidics mechanism 20 is mounted for vertical movement and has one center syringe-type needle and two outer syringe-type needles 24 disposed above the carousel 12 between the carousel inner and outer walls. When a container 14 is indexed such that it is disposed beneath the fluidics mechanism 20, the mechanism lowers the needles 24, 25 to access cavities in the cartridge 18 or package 16. As is later described herein, the immunoassay testing procedure requires the fluidics mechanism needles 24 to continually interact 7 with a reagent.package 16 and at least one test cartridge 18.

Reagents are drawn from the reagent package 16 using an aspiration pump (not shown). The pump draws fluids into one of the two outer needles 24 and dispenses it into the test cartridge 18 through the same needle 24.

The center needle 25 is an aspiration needle that provides an air path allowing the outer needles 24 to draw in reagents. Reagents that have been dispensed into the test cartridge 18 are removed from the cartridge 18 by one of the outer needles 24 and aspirated into an external containment bottle (not shown) Spring loaded electrical probes 26 are disposed e .above the carousel 12 and mounted for engagement of electrical contacts at the top of the test cartridge.

Although electrical contacts at the top of the test cartridge are described, the contacts may be anywhere on the cartridge, such as the cartridge side, that is eee accessible by probes adapted for the contact location.

The probes 26 apply an electrical current to the :electrical contacts for measuring electrical resistivity of circuitry on a resistor sheet 1'03 mounted in the test cartridge 18.

In one form of the assay, before testing begins, the probes 26 engage the contacts on the test cartridge 18 to determine a base resistivity. Upon completion of the immunoassay test, the test cartridges 18 are again indexed beneath the probes 26 for engagement with the contacts to determine whether a change in resistivity has occurred.

c -r i ~~nx 8 A microprocessor based control system (not shown) controls the movement of all mechanisms in the testing device 10 and accepts inputs, such as expiration dates, container identifiers, and test procedures, for processing and display from an internal bar code reader 27, the floppy disk drive 5, and an optional external bar code reader. The control system verifies that the correct reagent packages 16 will be used for the assigned cartridges 18 and that the containers 14 are not used 10 past their expiration date. Additionally, the test ooooo procedure identified for the test cartridge specimen contained within the cartridge 18 is provided to the control system and displayed to the user on the display 6.

The internal bar code scanner 27 reads expiration dates and container identifiers that are encoded on a bar *code mounted on the containers 14 and provides this information to the control system for verification and, S"if appropriate, display. The internal bar code scanner 27 mounted adjacent to the carousel 12 reads the external bar code mounted on the rear wall of each container 14.

bar code is mounted by adhesiGes or the like and identifies the container 14 to the control system.

The optional external bar code reader (not shown) is connected to the control system by a standard interface known in the art, such as an RS-232 connection. The external bar code reader reads a second bar code affixed to a container sidewall and identifies the container and specimen for user tracking.

9 A generally trapezoidal reagent package 16 shown in Figs. 4-6 has a base 30 and a top 32. A penetratable film (not shown), such as foil, protects the reagents from contamination and spilling during shipment.

Preferably, the reagent package 16 is molded from a plastic material that does not react with reagents contained therein, such as polypropylene, polyethylene or the like. -Light sensitive reagents are protected by a non-light transmissive color of the container, such as 1 0 black.

o* The base 30, as shown in Fig. 5, has a front wall 34, rear wall 36, and two sidewalls 38. Preferably, the base 30 is a segment of an annulus. The annulus is ooooo defined by the carousel inner and outer walls 15 and 17.

The base front and rear walls 34 and 36 are shaped to conform with the carousel walls 15 and 17. The base sidewalls 38 provide exterior flat surfaces for affixing labels or bar codes by adhesive or other means.

oei Tabs 40 formed in the front and rear walls 34 and 36 are received by corresponding indents 42 formed in the carousel inner and outer walls 15 and 17. The tabs and corresponding indents 42 ensure proper positioning of the package 16 on the carousel 12 as it is indexed beneath the fluidics mechanism 20 and to other positions in the testing device 10. Although, tabs 40 on the package front and rear wall 34 and 36 are described, other positioning mechanism may be used, such as indents in the cartridge and tabs on the carousel. Additionally, alternatives such as, one or more tabs, indents, or 10 combination thereof may be used on only the front wall or rear wall, rather than on both walls.

The reagent package top 32, shown in Figs. 4 and 6, is flat with a plurality of cavities 53-75 formed therein. The top 32 extends past the front and rear walls 34 and 36 of the base 30 providing finger holds for lifting and inserting the package 16 in and out of the carousel 12. The top 32 extending past the rear wall has six indents 46 formed therein strategically located for optically sensing the container position relative to the cavities 44. Although, indents sensed by an optical S"sensor are described, other locating mechanisms known in ~the art may be used.

Looking particularly at Fig. 6, the reagent package has eighteen upwardly facing cavities 53-75 arranged in three rows for various uses. The first row 48 and third row 50 have six cavities each with volumetric capacities for containing volumes of liquid reagents ranging from 385 to, 1600 microliters. The differing volumetric capacities eliminates wasting unused reagents when the cavity volume is sized for the specific reagents used.

o.o: The second row 52 of cavities disposed between the first and third row 48 and 50 contain distilled water for cleaning the needles 24 to eliminate any cross contamination of fluid during the testing procedure.

Each cavity in the center row 52 is a distillation well having a volumetric capacity of 900 microliters. Each time an outer needle 24 draws a reagent from the reagent package 16, the center needle 25 is dipped into the distilled water in a dilution well.

11 The specificity of the liquids contained within the cavities is determined by the specific test that will be conducted. In one embodiment in which the reagent container 16 is used in testing for the presence of a pathogen, such as Escherichia coli, cavity 53 has a volume of 500 microliters and is empty, cavity contains approximately 700 microliters of a buffer, such as tris(hydroxymethyl)aminomethane (generally known in the art as Tris), cavity 57 contains approximately 700 microliters of hydroquinone, cavity 59 contains approximately 700 microliters of distilled water, cavity 61 has a volume of 700 microliters and is empty, cavity 63 contains approximately 700 microliters of goldconjugated antibody, cavity 65 contains approximately 1600 microliters of Tris, cavity 67 contains approximately 1600 microliters of distilled HO0, cavity 69 contains approximately 385 microliters of silver lactate, cavity 71 contains approximately 1600 microliters of distilled H20, cavity 73 contains approximately 1600 microliters of distilled H20, and cavity 75 contains approximately 1600 microliters of Tris. The above volumes are approximate and used ,for illustration purposes. Different reagents and different volumes of the reagent may be substituted without departing from the scope of the present invention.

Preferably, specific reagents are provided in the reagent package sealed by a sheet of foil for use by the technician. A prepackaged reagent package eliminates the potential for contamination. Waste is reduced by providing the fluids in specific quantities sufficient 12 for a specific test of a specific number of test specimens.

Preferably, one or more test cartridges 18 are inserted into the carousel 12 adjacent to the reagent package 16 along an axis of motion, as shown in Figs. 1- 3. Preferably, the first test cartridge is directly adjacent to the reagent package sidewall. Additional test cartridges are directly adjacent to the test cartridges being serviced by the reagent package. It is preferred, 10 but not required, to place the reagent package and test ooeoo .cartridges serviced by the reagent package directly adjacent to each other along the axis of motion.

Placement of the test cartridges adjacent to the reagent o:ooo package reduces the potential for contamination or spillage of transferred fluids.

As shown in Figs. 7 and 8, a generally trapezoidal eo.. test cartridge has a base 81 and a top 83. A front Wall and rear wall 87 of the base 81 are shaped to conform with the carousel tray inner and outer wall 15 and 17.

Base sidewalls 89 provide exterior flat surfaces for affixing labels or bar codes by adhesive or other.means.

e Round tabs 91 formed in the front and rear walls 83 and 85 are received by corresponding indents 42 in the inner wall 15 and outer wall 17 of the carousel 12. The tabs 89 and corresponding indents 42 ensure proper positioning of the cartridge 18 as it is indexed by the carousel 12. Alternative positioning mechanisms may be used to position the container as described for the first embodiment.

:ii ^Li u 1 ~i 13 The test cartridge top 83, shown in Fig. 7, is flat with a single upwardly facing cavity 93 therein. The cartridge top 83 extends past the base front and rear walls 85 and 87 providing finger holds for lifting and inserting the cartridge 18 in and out of the carousel. A single indent 86 in the top portion extending past the rear wall 87 allows the optical sensing unit 22 to determine the cartridge 18 position on the carousel 12.

Again, alternatives locating mechanisms may be used position the container as described for the reagent "package.

Looking particularly at Figs. 8 and 9, the test cartridge cavity 93 has a front wall 95, rear wall 97, a ooo• pair of sidewalls 99, and a bottom wall 101. The cavity top is substantially defined by the base walls 85, 87 and 89. The cavity front and rear walls 95 and 97 slope inwardly and downwardly towards a receptacle 104 formed in the cavity bottom wall 101. In one embodiment, chamfers 96 formed along the top of the cavity sidewalls 99 expose electrical contacts 105 on a resistor sheet 103 to the spring loaded electrical probes 26. Although a test cartridge having a resistor sheet mounted therein is described, other methods of joining the resistors to a test cartridge, such as molding the resistors having exposed contacts into the side of the cartridge, may be employed to practice the invention.

Preferably, the test cartridge 18 is molded plastic, such as an acrylic or the like, formed from two symmetrical halves joined together at a central plane 102. Although the cartridge 18 could also be a single I1 14 molded piece, joining two halves simplifies mounting resistor sheets 103 to interior cavity sidewalls 99 as described herein. The halves are joined by conventional means such as ultrasonic welding, bonding, or elastomer seal. Preferably, an elastomer seal, such as santoprene or epoxy, is used. However, even a more rigid seal such as cyanoacrylate will work.

A disposable resistor sheet 103, shown in Figs. 9 and 10, having circuitry for engagement by the testing 1Ge: 0 device 10 is adhesively mounted to the interior of each cavity sidewall 99. The resistor sheet 103 is, ~preferably, disposable mylar with conductors 108, such as •Too silver tracings or the like, that electrically connect oeo o contacts 105 at the top of the resistor sheet 103 to three parallel resistors 107, such as carbon black or the like, at the bottom. Each resistor 107 on a single sheet .r is substantially identical having a resistance of at least 150 ohms.

layer of antigen or binding agent, such as an antibody, deposited over each resistor 107 and a portion of the conductors 108 on each end of the resistor 107 provides a binding surface for a molecule or organism of interest in the test sample. Subsequent sequential application of reagents from the reagent pack 16 leads to the deposition of electrically conductive particles bypassing the resistor 107 creating a closed circuit. The reaction chemistry and various antigen-antibody combinations are disclosed in U.S. Patent no. 5,284,748, which has been incorporated herein by reference.

15 It should be understood, however, that only one resistor sheet 103 is necessary to perform the test. By mounting a resistor sheet 103 on each cavity interior sidewall 99, a test for two different types of bacteria, such as Escherichia coli and Salmonella, may be performed on the same sample simultaneously. Upon completion of the test, the resistivity of the three resistors is averaged to maintain test reliability. Three resistors on a single resistor sheet are preferred, however, only a single resistor is necessary to practice the invention.

06.60.

In use, the external bar code affixed to the sidewall of the cartridge is read by the external bar code reader. The cartridge then is placed on the carousel *6oo 12 of the testing device 10. The test cartridge 18 is then indexed by the carousel 12 past the internal bar code reader and then beneath the spring loaded electrical contacts 26. The contacts 26 engage the electrical *6ee contacts 105 on the resistor sheet 103 in the cartridge ~18 to 'obtain a base reading prior to testing.

Once the base reading is obtained, the cartridge is indexed to a position accessible by the user and the user adds a specimen to the cartridge ,by pipetting or other 6 methods of adding a specific volume known in the art. The carousel 12 then indexes the reagent package beneath the fluidics mechanism 20. The mechanism 20 lowers the needles 24, 25 piercing the foil film covering the reagent package 16. The containers 14 are alternately indexed beneath the fluidics mechanism 20 allowing the needles 24 to transfer reagents between the reagent package 16 and test cartridge 18. Diluting fluid, such i i 16 as distilled water, contained in one or more of the reagent package cavities 44 is used to cleanse the aspiration needle 25 between transfers to avoid contamination.

EXAMPLE I In the following example, the automated immunoassay device 10 performs the test for Escherichia coli present in a test..specimen. Preferably, the carousel 12 contains at least one reagent package 16 having three adjacent test cartridges 18 and the testing device 10 follows the following procedure: 1) Read resistivity of each resistor sheet; 2) Add bacterial sample to each test cartridge, 3) Incubate test cartridges for 60 minutes; 4) Pick up 113 '1 of Tris from cavity *oo* 5) Aspirate needles into the containment bottle; 6) Pick up 113 1l of Tris from cavity 65 and dispense into test cartridge; repeat for each 550 test cartridge; 7) Aspirate Tris from each test cartridge into the containment bottle; 8) Pick up 113 1 of Tris from cavity 65 and dispense into test cartridge; repeat for each test cartridge; 9) Aspirate Tris from each test cartridge into the containment bottle; Pick up 140 ul Gold-conjugated antibody from cavity 63 and dispense into test cartridge; repeat for each test cartridge; n 17 11) Incubate test cartridges for 30 minutes; 12) Pick up 110 u1 of Tris from cavity 13) Aspirate Tris from each test cartridge into the containment bottle; 14) Pick up a 110 gl of Tris from cavity 55 and dispense into test cartridge; repeat for each test cartridge; Aspirate Tris from each test cartridge into the containment bottle; 10 16) Pick up 150 rl distilled H 2 0 from cavity 59 and dispense into test cartridge; repeat-for each test cartridge; 17) Aspirate distilled H20 from each test cartridge into the containment bottle; 15 18) Pick up 150 1l distilled H 2 0 from cavity 67 and dispense into test cartridge; repeat for each test cartridge; 19) Aspirate distilled HO from each test cartridge into the containment bottle; 20) Pick up 150 1l distilled H20 from cavity 67 and 0 dispense into test cartridge; repeat for each test cartridge; 21) Aspirate distilled H 2 0 from.each test cartridge into the containment bottle; 22) Pick up 170 A1 Hydroquinone from cavity 57 and dispense into test cartridge; repeat for each test cartridge; 23) Pick up 45 l Silver Lactate from cavity 69 and dispense into test cartridge; repeat for each test cartridge; :1 I; ~1 4" 18- 24) Mix solution in each test; Incubate each test cartridge 10 minutes; 26) Pick up 170 ul distilled H 2 0 from cavity 71; 27) Aspirate needles into containment bottle; 28) Pick up 170 Al distilled H 2 0 from cavity 71 and dispense into test cartridge; repeat for each test cartridge; 29) Aspirate distilled H 2 0 from each test cartridge into the containment bottle; i 30) Pick up 200 ul distilled H 2 0 from cavity 73 and S. dispense into test cartridge; repeat-for each e..

test cartridge; 31) Aspirate distilled H 2 0 from each test cartridge into the containment bottle; 32) Dry test cartridges for 10 minutes; 0

*SS.

Following the transfer of reagents and incubation periods required by the testing procedure, the carousel 12 indexes the test cartridges 18 for engagement with the spring loaded electrical contacts 26. The contacts 26 oooo oo engage the electrical contacts 105 on each resistor sheet o*

I

103 in the test cartridge 18 to obtain a final reading prior to testing. In the preferred embodiment, an electrical current is applied to each of the three circuits containing a single resistor to determine whether the circuit is open or closed for each resistor sheet. If the circuit is closed, the final resistance in the circuit is measured.

If the test is positive, that is the final resistance has dropped a defined percentage from the base 19 reading, a binding reaction occurred which is described fully in U.S. Patent 5,284,748 and fully incorporated herein by reference. In this example, the binding reaction as evidenced by the closed circuit provides an indication of the presence of Escherichia coli in the test specimen.

While there has been shown and described what are at present considered the preferred embodiment of the invention, it will be apparent to those skilled in the 10 art that various changes and modifications can be made therein without departing from the scope of the invention defined by the appended claims.

*ooo*

Claims (12)

1. A testing kit for use in an immunoassay testing device that detects a binding reaction between a pair of first and second substances that specifically bind together to form electrically conductive particles which fully or partially complete an open electrical circuit, said device having a conveying mechanism with a plurality of predetermined container positions, said kit comprising: a first container receivable by said conveying mechanism having at least three 1o groups of cavities, each group comprising a plurality of cavities for receiving fluids, at least one of said fluids is one of said substances. at least one second container receivable by said conveying mechanism to be S "displaced along the axis of motion of said conveying mechanism, said second container including at least one upwardly opening reaction cavity defined by front, rear and side oooi 15 walls joined by a bottom; at least one pair of electrical contacts which extend into said reaction cavity and define open ends of a circuit, wherein transferring at least one of said fluids from said first container to said reaction cavity causes a binding reaction in the presence of said other substance to form electrically conductive particles which electrically connect said at least 20 one pair of electrical contacts.

2. A testing kit as in claim 1 wherein each of said at least three groups of cavities in the first container comprises six cavities.

3. A testing kit as in claim 1 wherein surfaces of the fluid-receiving cavities of the first container do not react chemically with said fluids to interfere with the binding reaction between the substances.

4. A testing kit as in claim 1 wherein at least one cavity of the first container contains one of the reagents selected from the group consisting of a gold- conjugated antibody, hydroquinone, Tris, water, and silver lactate.

A testing kit as in claim 1 wherein the second container electrical contacts are on a first resistor sheet having a resistor, and the resistor sheet is mounted to one of the walls in the reaction cavity. [R:\LIBLL]12381.doc:caa ia-

6. A testing kit as in claim 1 wherein the front wall and the rear wall of said reaction cavity extend inwardly and downwardly toward a receptacle at the bottom of said reaction cavity.

7. A testing kit as claimed in claim 1 wherein an opposing pair of said reaction cavity walls define sloping surfaces which extend inwardly and downwardly toward said bottom, and said reaction cavity walls are joined to said bottom to form a fluid-tight internal bottom.

8. A container for use in an immunoassay testing device that detects a binding reaction between a pair of first and second substances that specifically bind together to form electrically conductive particles which fully or partially complete an open electrical circuit, said second container comprising: S• 15 at least one upwardly opening reaction cavity defined by front, rear and side walls joined by a bottom; S. at least one pair of electrical contacts which extend into said reaction cavity and S define open ends of a circuit, wherein transferring at least one substance to said reaction cavity causes a binding reaction in the presence of another substance to form electrically conductive particles which electrically connect said at least one pair of electrical contacts.

9. The container as in claim 8 in which the electrical contacts are on a first e resistor sheet having a resistor, and the resistor sheet is mounted to one of the walls in the reaction cavity.

The container as in claim 8 in which the front wall and the rear wall of said reaction cavity extend inwardly and downwardly toward a receptacle at the bottom of said reaction cavity.

11. The container as claimed in claim 8 in which an opposing pair of said reaction cavity walls define sloping surfaces which extend inwardly and downwardly toward said bottom, and said reaction cavity walls are joined to said bottom to form a fluid-tight internal bottom. [R:\LIBLL] 1238 l.doc:ca

12. A container for use in an immunoassay testing device substantially as hereinbefore described with reference to the accompanying drawings. Dated 29 January, 2002 Molecular Circuitry, Inc. Patent Attorneys for the Applicant/Nominated Person SPRUSON FERGUSON o* [R:\LIBLL]12381.doc:caa I 1