ATE93893T1 - EXPRESSION SYSTEMS FOR OVERPRODUCTION OF DESIRED PROTEINS. - Google Patents

EXPRESSION SYSTEMS FOR OVERPRODUCTION OF DESIRED PROTEINS.

Info

Publication number
ATE93893T1
ATE93893T1 AT86906582T AT86906582T ATE93893T1 AT E93893 T1 ATE93893 T1 AT E93893T1 AT 86906582 T AT86906582 T AT 86906582T AT 86906582 T AT86906582 T AT 86906582T AT E93893 T1 ATE93893 T1 AT E93893T1
Authority
AT
Austria
Prior art keywords
expression systems
overproduction
desired proteins
hosts
high yields
Prior art date
Application number
AT86906582T
Other languages
German (de)
Inventor
Bernard Allet
Eric H Kawashima
Original Assignee
Biogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Inc filed Critical Biogen Inc
Application granted granted Critical
Publication of ATE93893T1 publication Critical patent/ATE93893T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/191—Tumor necrosis factors [TNF], e.g. lymphotoxin [LT], i.e. TNF-beta
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/21—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Recombinant DNA molecules comprising DNA sequences derived from bacteriophase T4 that are useful in expressing desired polypeptides in unexpectedly high yields, hosts and expression systems comprising such recombinant DNA molecules and methods for expressing desired polypeptides in high yields by the utilization of such hosts and expression systems.
AT86906582T 1985-10-09 1986-10-09 EXPRESSION SYSTEMS FOR OVERPRODUCTION OF DESIRED PROTEINS. ATE93893T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US78584785A 1985-10-09 1985-10-09
EP86906582A EP0241539B1 (en) 1985-10-09 1986-10-09 Expression systems for overproduction of desired proteins
PCT/US1986/002133 WO1987002384A1 (en) 1985-10-09 1986-10-09 Expression systems for overproduction of desired proteins

Publications (1)

Publication Number Publication Date
ATE93893T1 true ATE93893T1 (en) 1993-09-15

Family

ID=25136805

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86906582T ATE93893T1 (en) 1985-10-09 1986-10-09 EXPRESSION SYSTEMS FOR OVERPRODUCTION OF DESIRED PROTEINS.

Country Status (6)

Country Link
EP (1) EP0241539B1 (en)
JP (1) JPS63500984A (en)
AT (1) ATE93893T1 (en)
CA (1) CA1297050C (en)
DE (1) DE3688974T2 (en)
WO (1) WO1987002384A1 (en)

Also Published As

Publication number Publication date
EP0241539A4 (en) 1989-04-24
DE3688974T2 (en) 1993-12-09
JPS63500984A (en) 1988-04-14
EP0241539A1 (en) 1987-10-21
EP0241539B1 (en) 1993-09-01
CA1297050C (en) 1992-03-10
DE3688974D1 (en) 1993-10-07
WO1987002384A1 (en) 1987-04-23

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