ATE85650T1 - HEAT SHOCK PROMOTOR AND GEN. - Google Patents

HEAT SHOCK PROMOTOR AND GEN.

Info

Publication number
ATE85650T1
ATE85650T1 AT85302593T AT85302593T ATE85650T1 AT E85650 T1 ATE85650 T1 AT E85650T1 AT 85302593 T AT85302593 T AT 85302593T AT 85302593 T AT85302593 T AT 85302593T AT E85650 T1 ATE85650 T1 AT E85650T1
Authority
AT
Austria
Prior art keywords
gene
dna
heat shock
fragment
plant
Prior art date
Application number
AT85302593T
Other languages
German (de)
Inventor
Joe L Key
William B Gurley
Ronald T Nagao
Friedrich Schoeffl
Eva Czarnecka
Original Assignee
Lubrizol Genetics Inc
Univ Georgia Res Found
Univ Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lubrizol Genetics Inc, Univ Georgia Res Found, Univ Florida filed Critical Lubrizol Genetics Inc
Application granted granted Critical
Publication of ATE85650T1 publication Critical patent/ATE85650T1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Non-Silver Salt Photosensitive Materials And Non-Silver Salt Photography (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A bacterial strain, for example Escherichia coli, contains and replicates a recombinant DNA plasmid comprising a vector, a fragment of plant DNA controlling expression of a heat shock gene from an Agrobacterium strain inserted into a T-DNA fragment of a Ti-plasmid and a foreign gene or a soybean gene under control of said fragment of plant DNA. A suitable vector is pBR322, and examples of suitable foreign genes include a Z-gene coating for beta -galactosidase or a crystalline endotoxin gene. Also disclosed are methods for recognising plant cells transformed by T-DNA of Agrobacterium species by expressing a transformation recognition gene under control of a soybean heat shock gene promoter.
AT85302593T 1984-04-13 1985-04-12 HEAT SHOCK PROMOTOR AND GEN. ATE85650T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/599,993 US5447858A (en) 1984-04-13 1984-04-13 Heat shock promoter and gene
EP85302593A EP0159884B1 (en) 1984-04-13 1985-04-12 Heat shock promoter and gene

Publications (1)

Publication Number Publication Date
ATE85650T1 true ATE85650T1 (en) 1993-02-15

Family

ID=24401942

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85302593T ATE85650T1 (en) 1984-04-13 1985-04-12 HEAT SHOCK PROMOTOR AND GEN.

Country Status (6)

Country Link
US (1) US5447858A (en)
EP (1) EP0159884B1 (en)
JP (2) JPS60248176A (en)
AT (1) ATE85650T1 (en)
CA (1) CA1338010C (en)
DE (1) DE3587084T2 (en)

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WO2004044161A2 (en) * 2002-11-06 2004-05-27 Fraunhofer Usa Expression of foreign sequences in plants using trans-activation system
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US7692063B2 (en) * 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
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US7807874B2 (en) 2003-12-10 2010-10-05 Monsanto Technology Llc Stress tolerant plants and methods thereof
US7230162B2 (en) * 2003-12-19 2007-06-12 Monsanto Technology Llc CEP1 gene promoter sequences from Zea mays and methods of use
WO2005081905A2 (en) * 2004-02-20 2005-09-09 Fraunhofer Usa Inc. Systems and methods for clonal expression in plants
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BRPI0515263A (en) * 2004-09-13 2008-07-15 Monsanto Technology Llc promoter molecules for use in plants
EP1789539A2 (en) * 2004-09-14 2007-05-30 Monsanto Technology, LLC Promoter molecules for use in plants
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BRPI0613111A2 (en) 2005-07-08 2010-12-21 Univ Mexico Nacional Autonoma bacterial proteins with pesticidal activity
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CN112210592A (en) * 2020-10-16 2021-01-12 贵州大学 Method for screening heat shock response molecular label yebE of escherichia coli based on qRT-PCR technology
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Also Published As

Publication number Publication date
EP0159884A3 (en) 1987-11-25
EP0159884B1 (en) 1993-02-10
DE3587084D1 (en) 1993-03-25
US5447858A (en) 1995-09-05
DE3587084T2 (en) 1993-07-22
CA1338010C (en) 1996-01-30
JPH0775567A (en) 1995-03-20
JPS60248176A (en) 1985-12-07
EP0159884A2 (en) 1985-10-30

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