ATE60801T1 - Expressionsvektoren fuer alpha-amylase in bacillus subtilis, erhaltene staemme und verfahren zur herstellung von alpha-amylase. - Google Patents

Expressionsvektoren fuer alpha-amylase in bacillus subtilis, erhaltene staemme und verfahren zur herstellung von alpha-amylase.

Info

Publication number
ATE60801T1
ATE60801T1 AT86401063T AT86401063T ATE60801T1 AT E60801 T1 ATE60801 T1 AT E60801T1 AT 86401063 T AT86401063 T AT 86401063T AT 86401063 T AT86401063 T AT 86401063T AT E60801 T1 ATE60801 T1 AT E60801T1
Authority
AT
Austria
Prior art keywords
amylase
alpha
production
methods
expression vectors
Prior art date
Application number
AT86401063T
Other languages
English (en)
Inventor
Daniel Levin
Philippe Joyet
Louvencourt Laurence De
Stephane Aymerich
Reverend Brigitte Le
Michel Steinmetz
Henri Heslot
Original Assignee
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient filed Critical Centre Nat Rech Scient
Application granted granted Critical
Publication of ATE60801T1 publication Critical patent/ATE60801T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1055—Levansucrase (2.4.1.10)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT86401063T 1985-05-22 1986-05-20 Expressionsvektoren fuer alpha-amylase in bacillus subtilis, erhaltene staemme und verfahren zur herstellung von alpha-amylase. ATE60801T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8507676A FR2582316B1 (fr) 1985-05-22 1985-05-22 Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase
EP86401063A EP0205371B1 (de) 1985-05-22 1986-05-20 Expressionsvektoren für alpha-Amylase in Bacillus subtilis, erhaltene Stämme und Verfahren zur Herstellung von alpha-Amylase

Publications (1)

Publication Number Publication Date
ATE60801T1 true ATE60801T1 (de) 1991-02-15

Family

ID=9319462

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86401063T ATE60801T1 (de) 1985-05-22 1986-05-20 Expressionsvektoren fuer alpha-amylase in bacillus subtilis, erhaltene staemme und verfahren zur herstellung von alpha-amylase.

Country Status (4)

Country Link
EP (1) EP0205371B1 (de)
AT (1) ATE60801T1 (de)
DE (1) DE3677415D1 (de)
FR (1) FR2582316B1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU620026B2 (en) * 1987-02-27 1992-02-13 Genencor International, Inc. Molecular cloning and expression of genes encoding proteolytic enzymes
RU2091487C1 (ru) * 1987-02-27 1997-09-27 Гист-Брокейдс Н.В. Способ получения штамма bacillus, содержащего две копии последовательности днк, кодирующей фермент с гидролазной активностью
DE3824827A1 (de) * 1988-07-21 1990-02-01 Henkel Kgaa Plasmide zur erzeugung von alpha-amylase in bacillus
FR2650292B1 (fr) * 1989-07-27 1994-03-04 Institut Recherche Agronomique Procede de production de proteine a partir de souches de bacillus subtilis et procede de preparation desdites souches
FR2665178A1 (fr) * 1990-07-30 1992-01-31 Agronomique Inst Nat Rech Variants thermostables de l'alpha-amylase de bacillus licheniformis, leur procede de preparation et leur utilisation.
DE69230825T2 (de) * 1991-04-26 2000-11-30 Genencor International, Inc. Genexpression in bazillen

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2537602B2 (fr) * 1982-09-24 1986-10-24 Centre Nat Rech Scient Nouvel adn recombinant perfectionne utile dans la preparation d'a-amylase par bacillus subtilis
FR2542011B1 (fr) * 1983-03-01 1985-11-29 Centre Nat Rech Scient Vecteurs de clonage et d'expression du gene sacb et procede pour la preparation de la levansaccharase

Also Published As

Publication number Publication date
FR2582316B1 (fr) 1988-12-02
EP0205371A1 (de) 1986-12-17
DE3677415D1 (de) 1991-03-14
EP0205371B1 (de) 1991-02-06
FR2582316A1 (fr) 1986-11-28

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