ATE521717T1 - PARALLEL NUCLEIC ACID SEQUENCING METHODS - Google Patents

PARALLEL NUCLEIC ACID SEQUENCING METHODS

Info

Publication number
ATE521717T1
ATE521717T1 AT04768958T AT04768958T ATE521717T1 AT E521717 T1 ATE521717 T1 AT E521717T1 AT 04768958 T AT04768958 T AT 04768958T AT 04768958 T AT04768958 T AT 04768958T AT E521717 T1 ATE521717 T1 AT E521717T1
Authority
AT
Austria
Prior art keywords
sequencing
molecules
nucleic acid
acid sequencing
sequencing methods
Prior art date
Application number
AT04768958T
Other languages
German (de)
Inventor
Mikhail Shchepinov
Kalim Mir
Original Assignee
Isis Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation filed Critical Isis Innovation
Application granted granted Critical
Publication of ATE521717T1 publication Critical patent/ATE521717T1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Abstract

The present invention relates to a method of sequencing a target polynucleotide by enzymatic and/or chemical means. The sequencing method includes a method for characterizing multiple alleles in a sample, a method for comparing polynucleotide sequences. The method involves analysing molecules as members of an array. Many target polynucleotides or many segments of a single target polynucleotide can be sequenced simultaneously. In a preferred embodiment the method involves analysing individual molecules within an array and base calls are based on the signals from two or more molecules. A method to prevent non-specific signal in sequencing is also provided. The invention is readily automated, both for small-scale and large-scale operation and relevant algorithms and the composition of kits and systems are provided.
AT04768958T 2003-10-20 2004-10-20 PARALLEL NUCLEIC ACID SEQUENCING METHODS ATE521717T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0324456.3A GB0324456D0 (en) 2003-10-20 2003-10-20 Parallel DNA sequencing methods
PCT/GB2004/004432 WO2005040425A2 (en) 2003-10-20 2004-10-20 Nucleic acid sequencing methods

Publications (1)

Publication Number Publication Date
ATE521717T1 true ATE521717T1 (en) 2011-09-15

Family

ID=29559567

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04768958T ATE521717T1 (en) 2003-10-20 2004-10-20 PARALLEL NUCLEIC ACID SEQUENCING METHODS

Country Status (5)

Country Link
US (3) US20070219367A1 (en)
EP (4) EP2431481A1 (en)
AT (1) ATE521717T1 (en)
GB (1) GB0324456D0 (en)
WO (1) WO2005040425A2 (en)

Families Citing this family (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1791682B (en) 2003-02-26 2013-05-22 凯利达基因组股份有限公司 Random array DNA analysis by hybridization
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US20080026380A1 (en) * 2006-07-31 2008-01-31 Siddiqi Suhaib M Nucleotide analogs
US7405434B2 (en) * 2004-11-16 2008-07-29 Cornell Research Foundation, Inc. Quantum dot conjugates in a sub-micrometer fluidic channel
WO2006064199A1 (en) * 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
WO2006084132A2 (en) 2005-02-01 2006-08-10 Agencourt Bioscience Corp. Reagents, methods, and libraries for bead-based squencing
EP2620510B2 (en) 2005-06-15 2020-02-19 Complete Genomics Inc. Single molecule arrays for genetic and chemical analysis
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US20070099212A1 (en) * 2005-07-28 2007-05-03 Timothy Harris Consecutive base single molecule sequencing
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7785786B2 (en) * 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
DK1987159T4 (en) 2006-02-08 2020-11-16 Illumina Cambridge Ltd PROCEDURE FOR SEQUENCE OF A POLYNUCLEOTID TEMPLATE
US20070202521A1 (en) * 2006-02-14 2007-08-30 Applera Corporation Single Molecule DNA Sequencing Using Fret Based Dynamic Labeling
US8364417B2 (en) 2007-02-15 2013-01-29 454 Life Sciences Corporation System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
EP2578702A1 (en) * 2006-02-16 2013-04-10 454 Life Sciences Corporation System and method for correcting primer extension errors in nucleic acid sequence data
US20070196832A1 (en) * 2006-02-22 2007-08-23 Efcavitch J William Methods for mutation detection
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN101495654A (en) 2006-04-19 2009-07-29 阿普里拉股份有限公司 Reagents, methods, and libraries for gel-free bead-based sequencing
SG173398A1 (en) 2006-07-19 2011-08-29 Bionanomatrix Inc Nanonozzle device arrays: their preparation and use for macromolecular analysis
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111705A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by hybrid capture
US8315817B2 (en) * 2007-01-26 2012-11-20 Illumina, Inc. Independently removable nucleic acid sequencing system and method
US8612161B2 (en) * 2008-03-19 2013-12-17 Intelligent Biosystems Inc. Methods and compositions for base calling nucleic acids
US20100035268A1 (en) * 2007-02-21 2010-02-11 Joseph Beechem Materials and methods for single molecule nucleic acid sequencing
CA2682275C (en) 2007-03-28 2017-05-09 Bionanomatrix, Inc. Methods of macromolecular analysis using nanochannel arrays
US20100173347A1 (en) * 2007-04-02 2010-07-08 Brook Michael A Stabilized gold nanoparticles and methods of making the same
EP2165353B1 (en) * 2007-06-29 2017-08-30 Applied Biosystems, LLC Systems and methods for electronic detection with nanofets
US7635566B2 (en) * 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
WO2009052214A2 (en) 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
WO2009055508A1 (en) * 2007-10-22 2009-04-30 Life Technologies Corporation A method and system for obtaining ordered, segmented sequence fragments along a nucleic acid molecule
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
EP2489744B1 (en) * 2008-02-04 2014-09-03 Life Technologies Corporation Composition and method for sequencing nucleic acid
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
WO2010033267A2 (en) * 2008-05-06 2010-03-25 University Of Georgia Research Foundation, Inc. Structures, methods of making structures, multi-well array surface enhanced raman spectroscopy (sers) chips, methods of making, and methods of use
EP2318547B1 (en) 2008-06-30 2018-05-30 BioNano Genomics, Inc. Methods for single-molecule whole genome analysis
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
WO2017120148A1 (en) 2016-01-04 2017-07-13 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
US10759824B2 (en) * 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
WO2010059731A2 (en) 2008-11-18 2010-05-27 Bionanomatrix, Inc. Polynucleotide mapping and sequencing
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
DK2669387T3 (en) 2009-08-25 2016-09-19 Illumina Inc Methods of selection and amplification of polynucleotides
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
CA2778338A1 (en) * 2009-10-21 2011-04-28 Bionano Genomics, Inc. Methods and related devices for single molecule whole genome analysis
US9234239B2 (en) 2009-10-23 2016-01-12 Life Technologies Corporation Systems and methods for error correction in DNA sequencing
US10240194B2 (en) 2010-05-13 2019-03-26 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
WO2011156707A2 (en) 2010-06-11 2011-12-15 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
EP2588144B1 (en) 2010-07-02 2018-05-09 Ventana Medical Systems, Inc. Detecting targets using mass tags and mass spectrometry
KR20120042100A (en) * 2010-10-22 2012-05-03 주식회사 씨젠 Detection of target nucleic acid sequences using dual-labeled immobilized probes on solid phase
WO2012058459A2 (en) 2010-10-27 2012-05-03 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
CN106399472A (en) 2010-10-28 2017-02-15 生命技术公司 Chemically-enhanced primer compositions, methods and kits
EP2659408B1 (en) 2010-12-29 2019-03-27 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
US10146906B2 (en) 2010-12-30 2018-12-04 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
CN105861645B (en) 2011-04-08 2020-02-21 生命科技股份有限公司 Phase-protected reagent flow ordering for use in sequencing-by-synthesis
EP2714928B1 (en) * 2011-05-27 2017-08-02 Life Technologies Corporation Methods for manipulating biomolecules
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US9453258B2 (en) 2011-09-23 2016-09-27 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP3789502A1 (en) * 2012-05-02 2021-03-10 Ibis Biosciences, Inc. Dna sequencing
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10192024B2 (en) 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
EP2891709A4 (en) * 2012-08-30 2016-04-13 Hitachi High Tech Corp Nucleic acid analysis device
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
EP2970366B1 (en) * 2013-03-15 2019-01-16 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
WO2015051338A1 (en) 2013-10-04 2015-04-09 Life Technologies Corporation Methods and systems for modeling phasing effects in sequencing using termination chemistry
JP6500897B2 (en) * 2014-06-20 2019-04-17 コニカミノルタ株式会社 Sensor chip for surface plasmon resonance excitation-enhanced fluorescence spectroscopy (SPFS), SPFS measurement method, and kit for SPFS
CN104237203B (en) * 2014-09-28 2016-08-24 苏州大学 A kind of SERS sensor of quantitative detection water sample ion concentration of mercury and preparation method thereof
JP6722179B2 (en) 2014-10-10 2020-07-15 インヴァイティ コーポレイションＩｎｖｉｔａｅ Ｃｏｒｐｏｒａｔｉｏｎ Universal blocking oligo system for multiple capture reactions and improved hybridization capture method
US10676787B2 (en) 2014-10-13 2020-06-09 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
CA2964790C (en) * 2014-10-24 2023-02-21 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
CN107969138B (en) 2015-05-14 2022-04-12 生命科技公司 Bar code sequences and related systems and methods
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
CN114989235A (en) 2015-09-28 2022-09-02 哥伦比亚大学董事会 Design and synthesis of nucleotides based on novel disulfide linkers for use as reversible terminators for DNA sequencing by synthesis
CN105400781B (en) * 2015-11-12 2018-08-24 广东省生态环境与土壤研究所 A kind of double block molecule probes and its quickly detect nuclei aoid methods
CN108603227A (en) * 2015-11-18 2018-09-28 卡利姆·U·米尔 Super-resolution is sequenced
WO2017127759A1 (en) 2016-01-22 2017-07-27 The Regents Of The University Of California Formation and use of embedded solutions in nanoscale materials
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2018118997A2 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
CN108265112B (en) * 2016-12-30 2023-04-25 深圳市真迈生物科技有限公司 Method for controlling sequencing reaction, sequencing system and control device
EP3596099A4 (en) * 2017-03-06 2020-12-09 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
BR112019021889A2 (en) 2017-05-05 2020-05-26 Quantum-Si Incorporated SUBSTRATES HAVING MODIFIED SURFACE REACTIVITY AND ANTI-SCALING PROPERTIES IN BIOLOGICAL REACTIONS
WO2018213460A1 (en) * 2017-05-16 2018-11-22 Helix Nanotechnologies, Inc. Linear covalently closed vectors and related compositions and methods thereof
EP3658682A4 (en) 2017-07-24 2021-06-02 Quantum-si Incorporated High intensity labeled reactant compositions and methods for sequencing
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
CN109957607A (en) * 2017-12-14 2019-07-02 深圳先进技术研究院 A kind of detection probe and its application
CN113039191A (en) 2018-10-25 2021-06-25 奇异基因组学系统公司 Nucleotide analogs
JP2022516684A (en) 2019-01-08 2022-03-01 シンギュラー・ゲノミクス・システムズ・インコーポレイテッド Nucleotide cleavable linker and its use
JP2022518056A (en) 2019-01-23 2022-03-11 クアンタム－エスアイ インコーポレイテッド Reaction Compositions and Methods for High Intensity Labeled Sequencing
EP3927720A4 (en) 2019-02-19 2022-11-30 Ultima Genomics, Inc. Linkers and methods for optical detection and sequencing
CN114829669A (en) 2019-10-11 2022-07-29 宽腾矽公司 Gas phase surface modification
CN114868004A (en) * 2019-10-18 2022-08-05 小利兰·斯坦福大学董事会 Whole tissue sequencing on clinical and industrial scale
KR20220143847A (en) 2020-01-21 2022-10-25 퀀텀-에스아이 인코포레이티드 Compounds and methods for selective C-terminal labeling
TWI724764B (en) * 2020-01-21 2021-04-11 國立臺灣大學 Spectral imaging device
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
CN114350774A (en) * 2022-01-28 2022-04-15 赛纳生物科技(北京)有限公司 Detection method for chip surface solid phase primer shearing efficiency

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
ATE356221T1 (en) 1996-06-06 2007-03-15 Solexa Inc SEQUENCING BY LIGATION OF ENCODED ADAPTERS
DE69837913T2 (en) * 1997-04-01 2008-02-07 Solexa Ltd., Saffron Walden PROCESS FOR THE MAKING OF NUCLEIC ACID
US6309829B1 (en) * 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
DE69930310T3 (en) * 1998-12-14 2009-12-17 Pacific Biosciences of California, Inc. (n. d. Ges. d. Staates Delaware), Menlo Park KIT AND METHOD FOR THE NUCLEIC ACID SEQUENCING OF INDIVIDUAL MOLECULES BY POLYMERASE SYNTHESIS
AU3748800A (en) * 1999-03-16 2000-10-04 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU7086800A (en) * 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
AU1239901A (en) * 1999-10-29 2001-05-14 Stratagene Compositions and methods utilizing dna polymerases
WO2001051663A2 (en) * 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
WO2001068913A2 (en) * 2000-03-13 2001-09-20 Genset Nucleic acid detection method and system
GB0013276D0 (en) 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US20030064366A1 (en) * 2000-07-07 2003-04-03 Susan Hardin Real-time sequence determination
US6818907B2 (en) 2000-10-17 2004-11-16 The President And Fellows Of Harvard College Surface plasmon enhanced illumination system
AU2002231933A1 (en) 2001-01-30 2002-08-12 Solexa Ltd. The preparation of polynucleotide arrays
JP2004523243A (en) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー Method and apparatus for analyzing polynucleotide sequences by asynchronous base extension
EP1370690B1 (en) 2001-03-16 2012-03-14 Kalim Mir Arrays and methods of use
US20030054396A1 (en) * 2001-09-07 2003-03-20 Weiner Michael P. Enzymatic light amplification
WO2003068986A1 (en) * 2002-02-11 2003-08-21 Applied Research Systems Ars Holding N.V. Nucleotide detection by fluorescence resonance energy transfer and methods thereof
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US20040014096A1 (en) 2002-04-12 2004-01-22 Stratagene Dual-labeled nucleotides
WO2003089670A2 (en) 2002-04-22 2003-10-30 Lawler Joseph F Jr Reagents for monitoring nuclei acid amplification and methods of using same
EP1594987B1 (en) * 2003-02-21 2009-05-27 GeneForm Technologies Limited Nucleic acid sequencing methods, kits and reagents
WO2005047471A2 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. Improved fret efficiency methods

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EP2431481A1 (en) 2012-03-21
WO2005040425A3 (en) 2005-07-28
US20070219367A1 (en) 2007-09-20
EP2270206A2 (en) 2011-01-05
GB0324456D0 (en) 2003-11-19
EP2270206A3 (en) 2011-03-02
US20140162892A1 (en) 2014-06-12
EP1689881B1 (en) 2011-08-24
US20160040227A1 (en) 2016-02-11

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