ATE474063T1 - METHOD AND MEANS FOR ISOLATING AND CLEANING NUCLEIC ACIDS ON SURFACES - Google Patents

METHOD AND MEANS FOR ISOLATING AND CLEANING NUCLEIC ACIDS ON SURFACES

Info

Publication number
ATE474063T1
ATE474063T1 AT99923428T AT99923428T ATE474063T1 AT E474063 T1 ATE474063 T1 AT E474063T1 AT 99923428 T AT99923428 T AT 99923428T AT 99923428 T AT99923428 T AT 99923428T AT E474063 T1 ATE474063 T1 AT E474063T1
Authority
AT
Austria
Prior art keywords
nucleic acids
processes
aimed
pcr
isolating
Prior art date
Application number
AT99923428T
Other languages
German (de)
Inventor
Martin Weber
Guido Fuhrmann
Joachim Schorr
Original Assignee
Qiagen Ges Mit Beschraenkter H
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP1998/006756 external-priority patent/WO1999022021A1/en
Application filed by Qiagen Ges Mit Beschraenkter H filed Critical Qiagen Ges Mit Beschraenkter H
Application granted granted Critical
Publication of ATE474063T1 publication Critical patent/ATE474063T1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Detergent Compositions (AREA)
  • Cleaning Or Drying Semiconductors (AREA)

Abstract

The present invention involves new processes and equipment for the isolation and purification of nucleic acids on surfaces. The invention is aimed at processes which use surfaces, e.g. porous membranes, at which, out of the sample containing the nucleic acids, in a simple manner, nucleic acids can be immobilized and can be released by means of equally simple process steps, whereby the simple carrying out, according to the invention, particularly permits the performance of the processes fully automatically. A further aspect of the invention is aimed at the fact, that nucleic acids are bonded to an immobile phase, particularly a membrane, in such a way, that they can be released again from this phase without any complications in a following reaction step and, if applicable, can be used for further applications, e.g. restrictive digestion, RT, PCR or RT-PCR or in any other of the above mentioned analytical- or enzyme reactions. Finally, the invention is directed at a special isolation container with which the processes can be executed according to the invention.
AT99923428T 1998-10-23 1999-04-20 METHOD AND MEANS FOR ISOLATING AND CLEANING NUCLEIC ACIDS ON SURFACES ATE474063T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP1998/006756 WO1999022021A1 (en) 1997-10-23 1998-10-23 Method for isolating and purifying nucleic acids on surfaces
PCT/EP1999/002664 WO2000024927A1 (en) 1997-10-23 1999-04-20 Methods and means for isolating and purifying nucleic acids on surfaces

Publications (1)

Publication Number Publication Date
ATE474063T1 true ATE474063T1 (en) 2010-07-15

Family

ID=8167111

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99923428T ATE474063T1 (en) 1998-10-23 1999-04-20 METHOD AND MEANS FOR ISOLATING AND CLEANING NUCLEIC ACIDS ON SURFACES

Country Status (6)

Country Link
JP (2) JP2002528093A (en)
AT (1) ATE474063T1 (en)
AU (1) AU765375C (en)
CA (2) CA2771000A1 (en)
DE (1) DE59915184D1 (en)
DK (1) DK1121460T3 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8034562B2 (en) * 2003-05-28 2011-10-11 Qiagen Gmbh Method for obtaining plasmid-DNA by means of an aqueous biphasic system
JP4831725B2 (en) * 2004-02-13 2011-12-07 栄研化学株式会社 Simple nucleic acid extraction method
JP2006217839A (en) * 2005-02-09 2006-08-24 Fuji Photo Film Co Ltd Method for separating and purifying nucleic acid
JP5627162B2 (en) * 2005-10-05 2014-11-19 倉敷紡績株式会社 Nucleic acid separation and purification method and nucleic acid separation kit using the method
US20080102493A1 (en) * 2006-06-29 2008-05-01 Millipore Corporation Isolation of RNA and DNA from a biological sample
CN103620388B (en) 2011-06-27 2016-05-11 奥林巴斯株式会社 The detection method of intended particle
EP3307438B1 (en) * 2015-06-10 2020-08-05 Biocartis N.V. Improved detection of methylated dna

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
JPH02190200A (en) * 1989-01-19 1990-07-26 Sanyo Chem Ind Ltd Detection of nucleic acid fragment and equipment therefor
JPH0666B2 (en) * 1989-05-02 1994-01-05 倉敷紡績株式会社 Method for isolating and purifying DNA
JPH0667B2 (en) * 1989-06-08 1994-01-05 倉敷紡績株式会社 Aqueous composition for nucleic acid isolation
JP2898306B2 (en) * 1989-07-20 1999-05-31 扶桑薬品工業株式会社 Nucleic acid amplification / detection methods and kits
CA2025476A1 (en) * 1989-09-27 1991-03-28 Shan F. Ching Hydrophilic laminated porous membranes and methods of preparing same
US5136032A (en) * 1989-12-07 1992-08-04 Daicel Chemical Industries, Ltd. Method for separating phosphopolyol compounds using a separating agent
IT1240870B (en) * 1990-02-14 1993-12-17 Talent PROCEDURE FOR THE EXTRACTION AND PURIFICATION OF HUMAN GENOMIC DNA
JPH04187077A (en) * 1990-11-22 1992-07-03 Shimadzu Corp Apparatus for extraction and purification of nucleic acid
JPH04325099A (en) * 1991-04-25 1992-11-13 Mitsui Petrochem Ind Ltd Immobilization of nucleic acid onto membrane
CA2067711C (en) * 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
JPH0751099A (en) * 1993-08-11 1995-02-28 Toyobo Co Ltd Method for examining sequence of nucleic acid and examination apparatus
JPH08159932A (en) * 1994-12-06 1996-06-21 Meito Sangyo Kk Sampling instrument, measuring method using it and measuring kit
JPH08285849A (en) * 1995-04-14 1996-11-01 Mochida Pharmaceut Co Ltd Convenient measuring method and apparatus
JPH11509100A (en) * 1995-07-13 1999-08-17 イムノロジカル アソシエーツ オブ デンバー Self-contained device that integrates nucleic acid extraction, amplification and detection
JP3641301B2 (en) * 1995-08-09 2005-04-20 株式会社セルシード Stimulation response type separation material and separation purification method
WO1997014815A1 (en) * 1995-10-18 1997-04-24 Darwin Molecular Corp. Methods for preparing solid supports for hybridization and reducing non-specific background
JPH10257887A (en) * 1996-09-30 1998-09-29 Dainippon Printing Co Ltd Gene analyzer and analysis
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids

Also Published As

Publication number Publication date
JP2008220377A (en) 2008-09-25
DE59915184D1 (en) 2010-08-26
JP2002528093A (en) 2002-09-03
CA2771000A1 (en) 2000-05-04
CA2348054A1 (en) 2000-05-04
AU4031599A (en) 2000-05-15
AU765375C (en) 2004-04-08
DK1121460T3 (en) 2010-09-20
CA2348054C (en) 2012-06-19
AU765375B2 (en) 2003-09-18

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