ATE471381T1 - ANIMAL CELL EXPRESSION VECTOR HAVING AT LEAST ONE COPY OF MAR DNA SEQUENCES AT THE 3' END OF THE TRANSCRIPTION TERMINATION AREA OF A GENE AND METHOD FOR EXPRESSING A FOREIGN GENE USING THE VECTOR - Google Patents

ANIMAL CELL EXPRESSION VECTOR HAVING AT LEAST ONE COPY OF MAR DNA SEQUENCES AT THE 3' END OF THE TRANSCRIPTION TERMINATION AREA OF A GENE AND METHOD FOR EXPRESSING A FOREIGN GENE USING THE VECTOR

Info

Publication number
ATE471381T1
ATE471381T1 AT06716203T AT06716203T ATE471381T1 AT E471381 T1 ATE471381 T1 AT E471381T1 AT 06716203 T AT06716203 T AT 06716203T AT 06716203 T AT06716203 T AT 06716203T AT E471381 T1 ATE471381 T1 AT E471381T1
Authority
AT
Austria
Prior art keywords
gene
vector
expression vector
expressing
copy
Prior art date
Application number
AT06716203T
Other languages
German (de)
Inventor
Jong-Mook Kim
Hye-Jin Hong
Hyun-Joo Lee
Moon-Kyoung So
Soo-Young Lee
Jong-Moon Cho
Bok-Hwan Chun
Seung-Suh Hong
Myung-Sam Cho
Original Assignee
Celltrion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celltrion Inc filed Critical Celltrion Inc
Application granted granted Critical
Publication of ATE471381T1 publication Critical patent/ATE471381T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Abstract

Provided are an expression vector for an animal cell including a promoter, a cloning site or a polynucleotide encoding foreign product, and a transcription terminator, all of which are operably connected each other within the expression vector, in which at least one copy of human β-globin MAR sequence is attached to the 31 terminal of the transcription terminator, and a method of expressing a foreign gene using the expression vector.
AT06716203T 2005-03-04 2006-03-04 ANIMAL CELL EXPRESSION VECTOR HAVING AT LEAST ONE COPY OF MAR DNA SEQUENCES AT THE 3' END OF THE TRANSCRIPTION TERMINATION AREA OF A GENE AND METHOD FOR EXPRESSING A FOREIGN GENE USING THE VECTOR ATE471381T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20050018130 2005-03-04
PCT/KR2006/000753 WO2006093397A1 (en) 2005-03-04 2006-03-04 Expression vector for animal cell comprising at least one copy of mar dna sequences at the 3'terminal of transcription termination region of a gene and method for the expression of foreign gene using the vector

Publications (1)

Publication Number Publication Date
ATE471381T1 true ATE471381T1 (en) 2010-07-15

Family

ID=36941411

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06716203T ATE471381T1 (en) 2005-03-04 2006-03-04 ANIMAL CELL EXPRESSION VECTOR HAVING AT LEAST ONE COPY OF MAR DNA SEQUENCES AT THE 3' END OF THE TRANSCRIPTION TERMINATION AREA OF A GENE AND METHOD FOR EXPRESSING A FOREIGN GENE USING THE VECTOR

Country Status (6)

Country Link
US (1) US8772021B2 (en)
EP (1) EP1859046B1 (en)
KR (2) KR20060096383A (en)
AT (1) ATE471381T1 (en)
DE (1) DE602006014942D1 (en)
WO (1) WO2006093397A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201390461A1 (en) 2010-10-08 2013-09-30 Кадила Хелзкэр Лимитед VECTOR OF EXPRESSION FOR HIGHLY EFFICIENT EXPRESSION OF RECOMBINANT PROTEINS
JP5931174B2 (en) * 2011-03-30 2016-06-08 パンジェン バイオテク インコーポレイテッド Animal cell expression vector
KR101420274B1 (en) * 2011-06-13 2014-07-21 주식회사 종근당 Animal Expression Vectors Carrying CSP-B 5' SAR Factor and Methods for Preparing Recombinant Proteins Using the Same
CN102250952B (en) * 2011-06-17 2016-01-20 中国人民解放军军事医学科学院生物工程研究所 The carrier that mediate foreign gene is expressed at mammal cell with high efficient and application thereof
KR20140015999A (en) * 2012-07-27 2014-02-07 한화케미칼 주식회사 Novel mars and method for producing target protein using the same
EP3270960A4 (en) * 2015-03-20 2018-08-08 Bluebird Bio, Inc. Vector formulations
US10722571B2 (en) 2015-06-10 2020-07-28 Celltrion Inc. Rabies virus G protein epitope, and rabies virus neutralising binding molecule that binds specifically thereto
WO2019074292A2 (en) 2017-10-11 2019-04-18 (주)셀트리온 Expression cassette for production of high-expression and high-functionality target protein and use thereof
WO2019151632A1 (en) 2018-01-31 2019-08-08 (주)셀트리온 Binding molecule having neutralizing activity against middle east respiratory syndrome-coronavirus
WO2021062012A1 (en) 2019-09-25 2021-04-01 Emory University Use of klk10 and engineered derivatizations thereof
KR20210080071A (en) 2019-12-20 2021-06-30 (주)셀트리온 Expression cassette for expressing target protein highly and method for using the same
KR102205028B1 (en) 2020-03-22 2021-01-20 (주)셀트리온 A binding molecules able to neutralize SARS-CoV-2
KR20220013344A (en) 2020-07-24 2022-02-04 (주)셀트리온 A binding molecules able to neutralize coronavirus superfamily
KR20230101087A (en) 2021-12-29 2023-07-06 한국과학기술원 A binding molecules able to neutralize PROX1 protein

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6245974B1 (en) 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6177612B1 (en) 1998-07-31 2001-01-23 Her Majesty The Queen In Right Of Canada, As Represented By The Department Of Agriculture And Agri-Food Canada Matrix attachment regions
CA2343080A1 (en) 1998-09-29 2000-04-06 Pioneer Hi-Bred International, Inc. Mar/sar elements flanking rsyn7-driven construct
GB2342282B (en) 1998-10-08 2001-09-26 Notetry Ltd Changeover valve
KR100408844B1 (en) 2000-07-29 2003-12-06 한국산업기술평가원 Expression vector using for animal cell
US7259010B2 (en) 2000-12-15 2007-08-21 Pangen Biotech Inc. Expression vector for animal cell containing nuclear matrix attachment region of interferon beta
WO2003024199A2 (en) 2001-09-21 2003-03-27 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
US6933136B2 (en) 2002-09-20 2005-08-23 Novo Nordisk A/S Method for making recombinant proteins

Also Published As

Publication number Publication date
DE602006014942D1 (en) 2010-07-29
US8772021B2 (en) 2014-07-08
EP1859046B1 (en) 2010-06-16
WO2006093397A1 (en) 2006-09-08
EP1859046A1 (en) 2007-11-28
KR101076602B1 (en) 2011-10-26
EP1859046A4 (en) 2008-09-17
KR20060096383A (en) 2006-09-11
KR20070118563A (en) 2007-12-17
US20080102523A1 (en) 2008-05-01

Similar Documents

Publication Publication Date Title
ATE471381T1 (en) ANIMAL CELL EXPRESSION VECTOR HAVING AT LEAST ONE COPY OF MAR DNA SEQUENCES AT THE 3' END OF THE TRANSCRIPTION TERMINATION AREA OF A GENE AND METHOD FOR EXPRESSING A FOREIGN GENE USING THE VECTOR
ATE550430T1 (en) PROMOTER NUCLEIC ACID FROM BACTERIA OF THE GENUS CORYNEBACTERIUM, EXPRESSION CASSETTE COMPRISING THE PROMOTER, HOST CELL COMPRISING THE VECTOR AND METHOD FOR EXPRESSING A GENE USING THE CELL.
MX350551B (en) Isolated polypeptides, polynucleotides encoding same, transgenic plants expressing same and methods of using same.
DE60322214D1 (en) "FROG-PRINCE" - A TRANSPOSANVEKTOR FOR THE TRANSFER OF TRANSFER IN SWIRLS
MX2008010669A (en) Plants having enhanced yield-related traits and a method for making the same.
RU2010116782A (en) SYNTHETIC 5 UTR (NON-TRANSLATED AREAS), EXPRESSION VECTORS AND METHODS FOR INCREASING TRANSGENIC EXPRESSION
ATE342990T1 (en) SURFACE EXPRESSION VECTORS WITH PGSBCA, THE GENE CODING FOR POLY-GAMMA-GLUTAMAT SYNTHETASE, AND METHOD FOR EXPRESSING A TARGET PROTEIN ON THE SURFACE OF A MICRO-ORGANISM USING THE VECTOR
ATE473278T1 (en) METHOD AND COMPOSITIONS FOR PRODUCING RECOMBINANT PROTEINS USING A GENE FOR TRNA
ATE498685T1 (en) ANTISENSE OLIGONUCLEOTIDES FOR INDUCING EXON SKIPPING AND METHOD FOR THE USE THEREOF
ATE506443T1 (en) USE OF A NUCLEIC ACID TO PRODUCE A TRANSGENIC PLANT WITH IMPROVED TOLERANCE TO DROUGHT
EP2410059A3 (en) Plants overexpressing Golden2-like protein (GLK) having enhanced yield-related traits and a method for making the same
EP2615174A3 (en) Plants having enhanced yield-related traits and/or increased abiotic stress resistance, and a method for making the same
ATE402260T1 (en) RECOMBINANT LACTOFERRIN, METHOD FOR THE PRODUCTION THEREOF BY PLANTS AND USE THEREOF
MX2009008600A (en) Plants having enhanced yield-related traits and a method for making the same.
WO2007003409A3 (en) Plants having increased yield and a method for making the same
CN103820452B (en) A kind of sgRNA fragment and application thereof
BR9912498A (en) Expression of eukaryotic peptides in plant plastidium
ATE542913T1 (en) PROMOTOR VARIANTS FOR EXPRESSING GENES IN A FUNGAL CELL
ATE387497T1 (en) SEED-SPECIFIC 7S ALPHA PROMOTERS FOR EXPRESSING GENES IN PLANTS
DE60035892D1 (en) GENES AND METHOD FOR GROWTH MANIPULATION
MX2022001859A (en) Method for treating muscular dystrophy by targeting lama1 gene.
ATE535605T1 (en) AUTOINDUCABLE SODIUM PHOSPHATE SYMPORTER PROMOTER FROM PICHIA PASTORIS AND METHOD FOR PRODUCING RECOMBINANT PROTEIN USING THEREOF
EP2487245A3 (en) Method for increasing pathogen resistance in transgenic plants
Sadikoglou et al. Comparative analysis of internal ribosomal entry sites as molecular tools for bicistronic expression
ATE368121T1 (en) EXPRESSION CASSETTES FOR BIDIRECTIONAL TRANSGENIC EXPRESSION OF NUCLEIC ACIDS IN PLANTS

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties