ATE469968T1 - METHOD AND MEANS FOR MONITORING AND MODULATING GENE SILENCING - Google Patents

METHOD AND MEANS FOR MONITORING AND MODULATING GENE SILENCING

Info

Publication number
ATE469968T1
ATE469968T1 AT03707900T AT03707900T ATE469968T1 AT E469968 T1 ATE469968 T1 AT E469968T1 AT 03707900 T AT03707900 T AT 03707900T AT 03707900 T AT03707900 T AT 03707900T AT E469968 T1 ATE469968 T1 AT E469968T1
Authority
AT
Austria
Prior art keywords
monitoring
gene silencing
modulating gene
gene
modulating
Prior art date
Application number
AT03707900T
Other languages
German (de)
Inventor
Peter Waterhouse
Susan Wesley
Chris Helliwell
Original Assignee
Commw Scient Ind Res Org
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commw Scient Ind Res Org filed Critical Commw Scient Ind Res Org
Application granted granted Critical
Publication of ATE469968T1 publication Critical patent/ATE469968T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Mold Materials And Core Materials (AREA)

Abstract

Methods and means are provided for monitoring and modulating reduction of gene expression in eukaryotic organisms, using double stranded RNA comprising, in addition to the dsRNA region comprising nucleotide sequences homologous to the target gene, additional dsRNA regions designed to down regulate a second gene or which are unrelated to the target gene.
AT03707900T 2002-03-14 2003-03-12 METHOD AND MEANS FOR MONITORING AND MODULATING GENE SILENCING ATE469968T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36385202P 2002-03-14 2002-03-14
PCT/AU2003/000293 WO2003076620A1 (en) 2002-03-14 2003-03-12 Methods and means for monitoring and modulating gene silencing

Publications (1)

Publication Number Publication Date
ATE469968T1 true ATE469968T1 (en) 2010-06-15

Family

ID=27805294

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03707900T ATE469968T1 (en) 2002-03-14 2003-03-12 METHOD AND MEANS FOR MONITORING AND MODULATING GENE SILENCING

Country Status (10)

Country Link
US (2) US20030175783A1 (en)
EP (1) EP1490489B1 (en)
JP (1) JP2006503548A (en)
CN (1) CN1643147B (en)
AT (1) ATE469968T1 (en)
AU (1) AU2003212089B2 (en)
CA (1) CA2478910C (en)
DE (1) DE60332820D1 (en)
ES (1) ES2346645T3 (en)
WO (1) WO2003076620A1 (en)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070118315A (en) * 1999-04-21 2007-12-14 와이어쓰 Compositions for inhibiting the function of polynucleotide sequences
US7485715B2 (en) * 1999-06-18 2009-02-03 Ceres, Inc. Sequence-determined DNA encoding AP2 domain polypeptides
US7479555B2 (en) * 1999-07-21 2009-01-20 Ceres, Inc. Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity
GB9918061D0 (en) * 1999-07-30 1999-10-06 Univ Bath Modified plants
DE10212892A1 (en) * 2002-03-20 2003-10-09 Basf Plant Science Gmbh Constructs and methods for regulating gene expression
WO2004106517A1 (en) * 2003-06-03 2004-12-09 Benitec Australia Limited Double-stranded nucleic acid
AU2004243347B2 (en) * 2003-06-03 2009-04-09 Benitec Australia Limited Double-stranded nucleic acid
WO2005038040A2 (en) * 2003-10-14 2005-04-28 Ceres, Inc. Methods and compositions for altering seed phenotypes
AU2005222084B2 (en) * 2004-03-05 2010-04-22 Benitec, Inc. Multiple promoter expression cassettes for simultaneous delivery of RNAI agents
US7429692B2 (en) * 2004-10-14 2008-09-30 Ceres, Inc. Sucrose synthase 3 promoter from rice and uses thereof
WO2006047495A2 (en) 2004-10-21 2006-05-04 Venganza Inc Methods and materials for conferring resistance to pests and pathogens of plants
WO2008069878A2 (en) 2006-10-27 2008-06-12 Ceres, Inc. Modulating lignin in plants
WO2008076922A1 (en) * 2006-12-15 2008-06-26 Ceres, Inc. Modulation of plant protein levels
US9758790B2 (en) 2004-12-08 2017-09-12 Ceres, Inc. Modulating the level of components within plants
EP1866418A2 (en) * 2005-02-22 2007-12-19 Ceres Inc. Modulating plant alkaloids
US7312376B2 (en) * 2005-04-20 2007-12-25 Ceres, Inc. Regulatory regions from Papaveraceae
US8124839B2 (en) * 2005-06-08 2012-02-28 Ceres, Inc. Identification of terpenoid-biosynthesis related regulatory protein-regulatory region associations
WO2007041536A2 (en) * 2005-09-30 2007-04-12 Ceres, Inc. Modulating plant tocopherol levels
US20090178160A1 (en) * 2005-10-25 2009-07-09 Joon-Hyun Park Modulation of Triterpenoid Content in Plants
WO2007084926A2 (en) * 2006-01-17 2007-07-26 Biolex Therapeutics, Inc. Compositions and methods for humanization and optimization of n-glycans in plants
US20090060921A1 (en) * 2006-01-17 2009-03-05 Biolex Therapeutics, Inc. Glycan-optimized anti-cd20 antibodies
US20090304901A1 (en) * 2006-01-25 2009-12-10 Steven Craig Bobzin Modulating plant protein levels
US8222482B2 (en) * 2006-01-26 2012-07-17 Ceres, Inc. Modulating plant oil levels
US20070199090A1 (en) * 2006-02-22 2007-08-23 Nestor Apuya Modulating alkaloid biosynthesis
WO2007120989A2 (en) * 2006-02-24 2007-10-25 Ceres, Inc. Shade regulatory regions
US20090222957A1 (en) * 2006-04-07 2009-09-03 Ceres Inc. Regulatory protein-regulatory region associations related to alkaloid biosynthesis
US20100024070A1 (en) * 2006-05-15 2010-01-28 Steven Craig Bobzin Modulation of oil levels in plants
WO2007147068A2 (en) * 2006-06-14 2007-12-21 Ceres, Inc. Increasing uv-b tolerance in plants
WO2007149570A2 (en) * 2006-06-21 2007-12-27 Ceres, Inc. Modulation of protein levels in plants
US20130191941A1 (en) 2006-07-05 2013-07-25 Shing Kwok Modulating light response pathways in plants, increasing light-related tolerances in plants, and increasing biomass in plants
US8222388B2 (en) * 2006-11-22 2012-07-17 Ceres, Inc. Broadly expressing regulatory regions
BRPI0701172B1 (en) * 2007-02-05 2019-11-26 Empresa Brasileira De Pesquisa Agropecuaria Embrapa compositions and methods for modifying gene expression using the ubiquitin conjugation protein gene promoter from soybean plants
BRPI0701230B1 (en) 2007-02-05 2018-06-26 Empresa Brasileira De Pesquisa Agropecuária - Embrapa COMPOSITIONS AND METHODS FOR MODIFYING GENES EXPRESSION USING THE UBIQUITIN CONJUGATION PROTEIN GENE PROMOTOR OF COTTON PLANTS
BRPI0701826B1 (en) 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária spider web proteins nephilengys cruentata, avicularia juruensis and parawixia bistriata isolated from Brazilian biodiversity
US8419145B2 (en) * 2008-07-25 2013-04-16 Eastman Kodak Company Inkjet printhead and method of printing with multiple drop volumes
US8298794B2 (en) * 2008-10-09 2012-10-30 Ceres, Inc. Cinnamyl-alcohol dehydrogenases
US20100257634A1 (en) * 2009-04-03 2010-10-07 Venganza Inc. Bioassay for gene silencing constructs
PL2659771T3 (en) 2009-07-20 2019-05-31 Ceres Inc Transgenic plants having increased biomass
WO2011009182A2 (en) 2009-07-24 2011-01-27 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Isolated nucleic acid molecule, genetic construct, vector, transgenic cell, method for producing a transgenic cell and plant, isolated and purified polypeptide, biodegradable pesticide composition, pest control method, method for producing transgenic strains resistant to insect pests
US9441233B2 (en) 2010-05-06 2016-09-13 Ceres, Inc. Transgenic plants having increased biomass
US8822427B2 (en) 2010-10-27 2014-09-02 Harrisvaccines Methods and compositions to protect aquatic invertebrates from disease
US10004797B2 (en) 2010-10-27 2018-06-26 Harrisvaccines, Inc. Method of rapidly producing improved vaccines for animals
EP2632486A1 (en) 2010-10-27 2013-09-04 Harrisvaccines Inc Method of rapidly producing vaccines for animals
WO2012058223A1 (en) 2010-10-27 2012-05-03 Ceres, Inc. Transgenic plants having altered biomass composition
BR112013031723B1 (en) 2011-06-10 2020-10-13 Temasek Life Sciences Laboratory Limited polynucleotide construction, method for transforming a fungal cell and isolated promoter
BR122020026837B1 (en) 2011-11-02 2021-04-13 Ceres, Inc METHOD OF INCREASING PLANT Yield IN SOIL CONTAINING HIGH LEVELS OF AL3 +, METHOD OF INCREASING TOLERANCE IN A PLANT
BR112014013853A2 (en) 2011-12-09 2020-01-14 Ceres Inc use of an isolated nucleic acid, method of obtaining a transgenic plant cell, method of obtaining a transgenic plant, method of producing a plant, method of producing biomass, method of biomass processing, method of altering the composition of biomass , biomass composition modulation method, production method of a forage product
US10100321B2 (en) 2012-04-19 2018-10-16 Temasek Life Sciences Laboratory Limited Methods for increasing cotton fiber length
CN104583404A (en) * 2012-06-25 2015-04-29 美国陶氏益农公司 Insect resistant and herbicide tolerant soybean event pDAB9582.816.15.1
US9101100B1 (en) 2014-04-30 2015-08-11 Ceres, Inc. Methods and materials for high throughput testing of transgene combinations
CA3226788A1 (en) 2014-07-15 2016-01-21 Ceres, Inc. Methods of increasing crop yield under abiotic stress
EP3209775A4 (en) 2014-10-22 2018-09-12 Temasek Life Sciences Laboratory Limited Terpene synthases from ylang ylang (cananga odorata var. fruticosa)
CA3018306A1 (en) 2016-03-24 2017-09-28 Universiteit Leiden Methods for transfecting plants and for reducing random integration events
CN109414451B (en) * 2016-05-16 2022-08-12 德克萨斯大学系统董事会 Compositions for delivering tRNA as nanoparticles and methods of use thereof
EP3502259A1 (en) 2017-12-19 2019-06-26 Universiteit Leiden A combinational strategy for reducing random integration events when transfecting plants
EP3744849A1 (en) 2019-05-31 2020-12-02 Consejo Superior de Investigaciones Cientificas (CSIC) Method for modulating the gene silencing degree induced by trans-acting small interfering rnas
US11473086B2 (en) 2019-06-19 2022-10-18 Ut-Battelle, Llc Loss of function alleles of PtEPSP-TF and its regulatory targets in rice
MX2022001439A (en) * 2019-08-02 2022-05-06 Commw Scient Ind Res Org Rna molecules for modulating flowering in plants.

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5037745A (en) * 1986-04-01 1991-08-06 University Of Medicine And Dentistry Of New Jersey Plasmid for the overproduction of bacteriophage T3 RNA polymerase, transcription vectors that carry a promoter recognized by its polymerase, gene coding for T3 RNA polymerase and application of these plasmids
GB9210273D0 (en) * 1992-05-13 1992-07-01 Ici Plc Dna
SK287538B6 (en) * 1998-03-20 2011-01-04 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
AR020078A1 (en) * 1998-05-26 2002-04-10 Syngenta Participations Ag METHOD FOR CHANGING THE EXPRESSION OF AN OBJECTIVE GENE IN A PLANT CELL
CA2408326A1 (en) * 2000-06-23 2002-01-03 E.I. Dupont De Nemours And Company Recombinant constructs and their use in reducing gene expression
AU2001281024A1 (en) * 2000-08-02 2002-02-13 The Board Of Regents Of The University Of Nebraska Down-regulation of single genes and simultaneous down-regulation of multiple genes by nuclear localization of rna transcripts
AU2001297906B2 (en) * 2000-10-31 2007-07-19 Commonwealth Scientific And Industrial Research Organisation Method and means for producing barley yellow dwarf virus resistant cereal plants

Also Published As

Publication number Publication date
JP2006503548A (en) 2006-02-02
WO2003076620A1 (en) 2003-09-18
ES2346645T3 (en) 2010-10-19
CN1643147B (en) 2010-04-14
EP1490489B1 (en) 2010-06-02
EP1490489A1 (en) 2004-12-29
CN1643147A (en) 2005-07-20
EP1490489A4 (en) 2006-06-14
AU2003212089B2 (en) 2008-08-21
AU2003212089A1 (en) 2003-09-22
CA2478910A1 (en) 2003-09-18
DE60332820D1 (en) 2010-07-15
CA2478910C (en) 2012-08-21
US20140196160A1 (en) 2014-07-10
US20030175783A1 (en) 2003-09-18

Similar Documents

Publication Publication Date Title
DE60332820D1 (en) METHOD AND MEANS FOR MONITORING AND MODULATION OF THE GENE SILENCING
WO2004044181A3 (en) Antisense modulation of apolipoprotein b expression
WO2003097662A8 (en) Antisense modulation of apolipoprotein b expression
WO2003011887A3 (en) Antisense modulation of apolipoprotein b expression
EA200600591A1 (en) EIF4E EXPRESSION MODULATION
DE602004017426D1 (en) USE OF INTRONIC RNA SEQUENCES FOR THE QUANTIFICATION OF GENE EXPRESSION
WO2005049630A3 (en) Antisense modulation of kinesin-like 1 expression
WO2002036743A3 (en) Antisense modulation of calreticulin expression
WO2005000201A3 (en) Modulation of apolipoprotein (a) expression
DE602005020691D1 (en) RNA AMPLIFICATION ON LIGATION BASIS
WO2004071407A3 (en) Antisense modulation of ptp1b expression
WO2004005460A3 (en) Antisense modulation of hmg-coa reductase expression
AU2001290706A1 (en) Antisense modulation of clusterin expression
WO2004016224A3 (en) Antisense modulation of vegf co-regulated chemokine-1 expression
WO2003046132A3 (en) Antisense modulation of myd88 expression
WO2004055162A3 (en) Modulation of endothelial lipase expression
WO2003105755A3 (en) Antisense modulation of vegf-c expression
WO2001083513A3 (en) Antisense modulation of inhibitor of dna binding-1 expression
WO2004010956A3 (en) Antisense modulation of lar expression
WO2005014607A3 (en) Antisense modulation of stearoyl-coa desaturase expression
ATE420975T1 (en) METHOD FOR LINEAR NON-SELECTIVE AMPLIFICATION OF NUCLEIC ACIDS
WO2002046367A3 (en) Antisense modulation of cellular apoptosis susceptibility gene expression
WO2004014299A3 (en) Antisense modulation of resistin expression
WO2003050247A3 (en) Antisense modulation of mhc class ii transactivator expression
WO2003099204A3 (en) Antisense modulation of inhibitor-kappa b kinase-beta expression

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties