ATE467681T1 - COMPOSITIONS AND METHODS OF USING A SYNTHETIC DNASE I - Google Patents
COMPOSITIONS AND METHODS OF USING A SYNTHETIC DNASE IInfo
- Publication number
- ATE467681T1 ATE467681T1 AT04749602T AT04749602T ATE467681T1 AT E467681 T1 ATE467681 T1 AT E467681T1 AT 04749602 T AT04749602 T AT 04749602T AT 04749602 T AT04749602 T AT 04749602T AT E467681 T1 ATE467681 T1 AT E467681T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- dnase
- compositions
- methods
- present
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Compositions and methods for making and using a synthetic bovine DNase T are disclosed. More particularly, the sbDNaseI of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5'-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clcan-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling culture cells; tissue dissociation creation of fragmented DNA for in vitro recombination reactions.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US40402303A | 2003-03-31 | 2003-03-31 | |
US10/420,345 US7067298B2 (en) | 2003-03-31 | 2003-04-22 | Compositions and methods of using a synthetic Dnase I |
PCT/US2004/009955 WO2004090114A2 (en) | 2003-03-31 | 2004-03-31 | Compositions and methods of using a synthetic dnase i |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE467681T1 true ATE467681T1 (en) | 2010-05-15 |
Family
ID=33161958
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT04749602T ATE467681T1 (en) | 2003-03-31 | 2004-03-31 | COMPOSITIONS AND METHODS OF USING A SYNTHETIC DNASE I |
Country Status (5)
Country | Link |
---|---|
US (3) | US7067298B2 (en) |
EP (2) | EP2213741A3 (en) |
AT (1) | ATE467681T1 (en) |
DE (1) | DE602004027123D1 (en) |
WO (1) | WO2004090114A2 (en) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2163652B2 (en) | 2002-01-28 | 2023-05-31 | Life Technologies Corporation | Preparing crude biological extracts using protease, suitable for preparing cDNA |
DE60319333D1 (en) * | 2002-12-20 | 2008-04-10 | Roche Diagnostics Gmbh | Heat labile deoxyribonuclease I variants |
WO2005093052A1 (en) * | 2004-03-26 | 2005-10-06 | Fuji Photo Film Co., Ltd. | Method for selectively separating and purifying rna and method for separating and purifying nucleic acid |
US20060223072A1 (en) * | 2005-03-31 | 2006-10-05 | Boyes Barry E | Methods of using a DNase I-like enzyme |
US20060223073A1 (en) * | 2005-03-31 | 2006-10-05 | Boyes Barry E | Methods of using a DNase I-like enzyme |
US20070141584A1 (en) * | 2005-12-20 | 2007-06-21 | Roberts Douglas N | Methods for assessment of native chromatin on microarrays |
US20080044851A1 (en) * | 2006-06-02 | 2008-02-21 | Epicentre Technologies | Compositions and methods for removal of DNA from a sample |
US8725258B2 (en) * | 2006-08-25 | 2014-05-13 | Cardiac Pacemakers, Inc. | Method and apparatus for automated adjustment of arrhythmia detection duration |
EP2086642B1 (en) | 2006-10-18 | 2014-06-25 | Periness Ltd. | Dnase for the treatment of male sub-fertility |
US11781127B2 (en) * | 2007-01-16 | 2023-10-10 | Alex Garvin | Homogeneous method to prepare sperm DNA from sexual assault cases |
US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
EP2294217A4 (en) * | 2008-05-23 | 2012-10-31 | Life Technologies Corp | Methods for removing nucleic acid contamination from reagents |
WO2010071833A1 (en) * | 2008-12-19 | 2010-06-24 | Life Technologies Corporation | Proteinase k inhibitors, methods and compositions therefor |
EP2416796A4 (en) * | 2009-04-10 | 2012-08-29 | Bio Rad Laboratories | Modified dnase compositions and methods of use thereof |
KR20240007725A (en) | 2009-11-02 | 2024-01-16 | 유니버시티 오브 워싱톤 스루 이츠 센터 포 커머셜리제이션 | Therapeutic Nuclease Compositions and Methods |
EP2538988B1 (en) | 2010-02-26 | 2019-01-30 | DeCell Technologies Inc. | Methods for tissue decellularization |
GB201101794D0 (en) | 2011-02-02 | 2011-03-16 | Fermentas Uab | Protein production |
EA201391585A1 (en) | 2011-04-29 | 2014-04-30 | Юниверсити Оф Вашингтон | THERAPEUTIC NUCLEASE COMPOSITIONS AND METHODS |
RU2502803C2 (en) * | 2011-05-20 | 2013-12-27 | Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") | PLASMID FOR EXPRESSION IN CELLS OF BACTERIUM BELONGING TO Escherichia CLASS, NON-ACTIVE PREDECESSOR OF DNase I OF HUMAN OR ITS MUTEINS; BACTERIUM BELONGING TO Escherichia CLASS, - PRODUCER OF NON-ACTIVE PREDECESSOR OF RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; PREDECESSOR OF RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; METHOD FOR OBTAINING RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; METHOD FOR OBTAINING CONJUGATES OF POLYETHYLENE GLYCOL AND RECOMBINANT MUTEIN OF HUMAN DNase I, FERMENTATIVE ACTIVE CONJUGATE OF MUTEIN OF RECOMBINANT DNase I OF HUMAN |
GB201217405D0 (en) | 2012-09-28 | 2012-11-14 | Fermentas Uab | Protein removal agent |
US10988745B2 (en) | 2013-10-31 | 2021-04-27 | Resolve Therapeutics, Llc | Therapeutic nuclease-albumin fusions and methods |
US10307510B2 (en) | 2013-11-04 | 2019-06-04 | Lifecell Corporation | Methods of removing alpha-galactose |
WO2016144891A1 (en) * | 2015-03-06 | 2016-09-15 | Bio-Rad Laboratories, Inc. | Stabilized rna solutions |
US10329623B2 (en) * | 2015-08-28 | 2019-06-25 | Slmp, Llc | Synthetic tissue controls and synthetic tissue microarray controls |
US10479981B2 (en) | 2015-10-14 | 2019-11-19 | Novozymes A/S | DNase variants |
AU2020215502A1 (en) | 2019-01-28 | 2021-08-19 | Becton, Dickinson And Company | Oligonucleotide-comprising cellular component-binding reagents and methods of using the same |
CN110819622A (en) * | 2019-10-09 | 2020-02-21 | 广州达正生物科技有限公司 | Method for rapidly extracting RNA (ribonucleic acid) of paraffin tissue |
EP4055156A2 (en) * | 2019-11-08 | 2022-09-14 | Thermo Fisher Scientific Baltics Uab | Deoxyribonuclease variants and uses thereof |
EP4347804A1 (en) * | 2021-05-27 | 2024-04-10 | New England Biolabs, Inc. | Dnase i variants, compositions, methods, and kits |
US11993792B2 (en) | 2021-05-27 | 2024-05-28 | New England Biolabs, Inc. | DNase I variants, compositions, methods, and kits |
CN114250208B (en) * | 2021-12-22 | 2023-07-25 | 武汉瀚海新酶生物科技有限公司 | Preparation and application of high-salt-resistant high-activity DNase I mutant |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
US5279823A (en) | 1992-06-08 | 1994-01-18 | Genentech, Inc. | Purified forms of DNASE |
GB9300686D0 (en) * | 1993-01-15 | 1993-03-03 | Imp Cancer Res Tech | Compounds for targeting |
US6251648B1 (en) * | 1998-04-03 | 2001-06-26 | Human Genome Sciences, Inc. | Gene encoding human Dnase |
US6391607B1 (en) | 1996-06-14 | 2002-05-21 | Genentech, Inc. | Human DNase I hyperactive variants |
CN1152134C (en) * | 2001-01-19 | 2004-06-02 | 北京华大蛋白质研发中心有限公司 | Process for preparing recombinant deoxyribonuclease I |
EP1433842B1 (en) * | 2002-12-18 | 2011-01-05 | Roche Diagnostics GmbH | Recombinant bovine pancreatic desoxyribonuclease I with high specific activity |
-
2003
- 2003-04-22 US US10/420,345 patent/US7067298B2/en not_active Expired - Lifetime
-
2004
- 2004-03-31 EP EP10153371A patent/EP2213741A3/en not_active Withdrawn
- 2004-03-31 DE DE602004027123T patent/DE602004027123D1/en not_active Expired - Lifetime
- 2004-03-31 EP EP04749602A patent/EP1608732B1/en not_active Expired - Lifetime
- 2004-03-31 AT AT04749602T patent/ATE467681T1/en not_active IP Right Cessation
- 2004-03-31 WO PCT/US2004/009955 patent/WO2004090114A2/en active Application Filing
-
2006
- 2006-02-23 US US11/361,739 patent/US20060228731A1/en not_active Abandoned
-
2011
- 2011-05-09 US US13/103,798 patent/US20110318811A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2004090114A2 (en) | 2004-10-21 |
EP1608732B1 (en) | 2010-05-12 |
EP2213741A2 (en) | 2010-08-04 |
DE602004027123D1 (en) | 2010-06-24 |
WO2004090114A3 (en) | 2006-03-30 |
EP1608732A4 (en) | 2007-09-26 |
US7067298B2 (en) | 2006-06-27 |
EP1608732A2 (en) | 2005-12-28 |
US20110318811A1 (en) | 2011-12-29 |
US20040219529A1 (en) | 2004-11-04 |
US20060228731A1 (en) | 2006-10-12 |
EP2213741A3 (en) | 2010-11-17 |
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Legal Events
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |