ATE434043T1 - Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arrays - Google Patents

Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arrays

Info

Publication number
ATE434043T1
ATE434043T1 AT05707166T AT05707166T ATE434043T1 AT E434043 T1 ATE434043 T1 AT E434043T1 AT 05707166 T AT05707166 T AT 05707166T AT 05707166 T AT05707166 T AT 05707166T AT E434043 T1 ATE434043 T1 AT E434043T1
Authority
AT
Austria
Prior art keywords
arrays
dna molecules
linear dna
active linear
interest
Prior art date
Application number
AT05707166T
Other languages
English (en)
Inventor
Khabar Khalid S Abu
Original Assignee
King Faisal Specialist Hospita
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by King Faisal Specialist Hospita filed Critical King Faisal Specialist Hospita
Application granted granted Critical
Publication of ATE434043T1 publication Critical patent/ATE434043T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
AT05707166T 2005-02-03 2005-02-03 Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arrays ATE434043T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2005/001078 WO2006081831A1 (en) 2005-02-03 2005-02-03 Method of generating translationally active linear dna molecules and use thereof in array formats

Publications (1)

Publication Number Publication Date
ATE434043T1 true ATE434043T1 (de) 2009-07-15

Family

ID=34960919

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05707166T ATE434043T1 (de) 2005-02-03 2005-02-03 Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arrays

Country Status (7)

Country Link
US (1) US20080305961A1 (de)
EP (1) EP1844143B1 (de)
AT (1) ATE434043T1 (de)
CA (1) CA2596758A1 (de)
DE (1) DE602005015028D1 (de)
DK (1) DK1844143T3 (de)
WO (1) WO2006081831A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008127450A2 (en) * 2006-12-04 2008-10-23 Vical Incorporated Linear expression cassette vaccines
WO2009155950A1 (en) * 2008-06-27 2009-12-30 King Faisal Specialist Hospital And Research Centre Cloning-free method of generating transcriptionally and post-transcriptionally controllable expression active linear reporter constructs
GB2483462A (en) 2010-09-08 2012-03-14 Mologen Ag Use of a dumbbell-shaped DNA construct for the treatment of cancer through jet-injection administration
WO2012147370A1 (ja) * 2011-04-28 2012-11-01 国立大学法人山口大学 ターミネータ配列を含む高発現用リバースプライマー及びリニアｄｎａ
CN214973877U (zh) 2019-08-09 2021-12-03 胡桃钳医疗公司 用于形成治疗性多核苷酸的微流体设备及微流体路径装置

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571690A (en) * 1991-05-08 1996-11-05 The University Of Virginia Patents Foundation Method for the cell-free synthesis of proteins
JP2003509060A (ja) * 1999-09-17 2003-03-11 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 逆トランスフェクション方法

Also Published As

Publication number Publication date
EP1844143A1 (de) 2007-10-17
DK1844143T3 (da) 2009-10-12
CA2596758A1 (en) 2006-08-10
WO2006081831A1 (en) 2006-08-10
US20080305961A1 (en) 2008-12-11
EP1844143B1 (de) 2009-06-17
DE602005015028D1 (de) 2009-07-30

Similar Documents

Publication Publication Date Title
WO2007053245A3 (en) Methods and composition to generate unique sequence dna probes, iabeling of dna probes and the use of these probes
WO2006135684A3 (en) Methods and kits for sense rna synthesis
WO2009076485A3 (en) Sequencing of nucleic acids
HK1091871A1 (en) Random array dna analysis by hybridization
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
WO2007040592A8 (en) Making nucleic acid sequences in parallel and use
WO2002072773A3 (en) Methods and compositions for amplification of rna sequences
WO2008069906A3 (en) Digital expression of gene analysis
EP1994142A4 (de) Verfahren und zusammensetzungen zur schnellen isolierung kleiner rna-moleküle
ATE521695T1 (de) Zirkuläres dna-molekül mit einem konditionalen replikationsursprung, verfahren zur herstellung davon und ihre verwendung in der gentherapie
CO6341638A2 (es) Axmi-115, axmi-113, axmi-005, axmi-163 y axmi-184: proteinas insecticidas y metodos para su uso
WO2009092035A3 (en) Methods and compositions for the analysis of biological molecules
ATE461292T1 (de) Erstellung von expressionsprofilen unter verwendung von mikroarrays
EA201171006A1 (ru) Вариантные гены axmi-r1 дельта-эндотоксина и способы их применения
ATE500331T1 (de) Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden
ATE446384T1 (de) Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates
EP2379577A4 (de) Zusammensetzungen und verfahren zur umprogrammierung von zellen ohne genetische modifikation
ATE434043T1 (de) Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arrays
BRPI0907629A2 (pt) enzimas e genes transportadores de açúcar termoacidofílico e termofílico de alicyclobacillus acidocaldarius e organismos relacionados e métodos
Floyd et al. Protein sequencing, one molecule at a time
ATE469984T1 (de) Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
WO2010039189A3 (en) Methods for sequencing degraded or modified nucleic acids
DK2066790T3 (da) Galactosyltransferase
WO2004101749A3 (en) Methods for amplification of nucleic acid sequences using staggered ligation
ATE399868T1 (de) Optimierte nukleotidsequenzen die für sgp130 kodieren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties