ATE408711T1 - POLYNOMIAL AMPLIFICATION OF NUCLEIC ACIDS - Google Patents
POLYNOMIAL AMPLIFICATION OF NUCLEIC ACIDSInfo
- Publication number
- ATE408711T1 ATE408711T1 AT03711339T AT03711339T ATE408711T1 AT E408711 T1 ATE408711 T1 AT E408711T1 AT 03711339 T AT03711339 T AT 03711339T AT 03711339 T AT03711339 T AT 03711339T AT E408711 T1 ATE408711 T1 AT E408711T1
- Authority
- AT
- Austria
- Prior art keywords
- amplification
- nucleic acids
- nucleic acid
- primer
- polynomial amplification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention provides methods and compositions for the amplification and replication of nucleic acid molecules. In particular, novel amplification methods, referred to herein as polynomial amplification, are provided. According to these methods, a nucleic acid molecule to be amplified is contacted with at least two primers; a non-replicable primer which may hybridize to the nucleic acid molecule being amplified, and a replicable primer which may hybridize to a primer extension product generated from extension of the non-replicable primer.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36099502P | 2002-03-01 | 2002-03-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE408711T1 true ATE408711T1 (en) | 2008-10-15 |
Family
ID=27789055
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03711339T ATE408711T1 (en) | 2002-03-01 | 2003-02-28 | POLYNOMIAL AMPLIFICATION OF NUCLEIC ACIDS |
Country Status (7)
Country | Link |
---|---|
US (1) | US7112406B2 (en) |
EP (1) | EP1481088B1 (en) |
AT (1) | ATE408711T1 (en) |
AU (1) | AU2003213652A1 (en) |
CA (1) | CA2476564C (en) |
DE (1) | DE60323611D1 (en) |
WO (1) | WO2003074724A2 (en) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7629152B2 (en) * | 2002-03-01 | 2009-12-08 | Integrated Dna Technologies, Inc. | Methods for amplifying polymeric nucleic acids |
US20030228596A1 (en) * | 2002-03-14 | 2003-12-11 | Qiang Liu | Template-driven nucleic acid amplifications |
EP1649065B1 (en) * | 2003-08-01 | 2010-03-03 | Integrated Dna Technologies, Inc. | Methods for amplifying polymeric nucleic acids |
US20070020667A1 (en) * | 2005-06-30 | 2007-01-25 | Ruff David W | Methods and compositions for amplifying nucleic acids |
US20090068643A1 (en) * | 2005-11-23 | 2009-03-12 | Integrated Dna Technologies, Inc. | Dual Function Primers for Amplifying DNA and Methods of Use |
WO2007106907A2 (en) * | 2006-03-15 | 2007-09-20 | Integrated Dna Technologies, Inc. | Nucleic acid monomers with 2'-chemical moieties |
EP2069540B1 (en) * | 2006-10-09 | 2014-03-19 | Oxitec Limited | Methods for amplifying and detecting nucleic acid sequences |
GB0703996D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Nucleic acid detection |
GB0703997D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Methods for detecting nucleic sequences |
RU2414510C2 (en) * | 2007-06-14 | 2011-03-20 | Институт биохимии и генетики Уфимского научного центра РАН | Method of amplifying specific nucleic acid fragments through recurrent chain reaction |
US8455197B2 (en) | 2007-10-04 | 2013-06-04 | Commonwealth Scientific And Industrial Research Organisation | Nucleic acid amplification |
US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
EP2644708B1 (en) | 2008-04-30 | 2014-12-17 | Integrated Dna Technologies, Inc. | RNASE-H-based assays utilizing modified RNA monomers |
WO2011060014A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
GEP20156313B (en) | 2010-09-22 | 2015-07-10 | Alios Biopharma Inc | Substituted nucleotide analogs |
AU2012358804B2 (en) | 2011-12-22 | 2018-04-19 | Alios Biopharma, Inc. | Substituted phosphorothioate nucleotide analogs |
CN104321333A (en) | 2012-03-21 | 2015-01-28 | 沃泰克斯药物股份有限公司 | Solid forms of a thiophosphoramidate nucleotide prodrug |
NZ630805A (en) | 2012-03-22 | 2016-01-29 | Alios Biopharma Inc | Pharmaceutical combinations comprising a thionucleotide analog |
WO2014152054A1 (en) | 2013-03-15 | 2014-09-25 | Bio-Rad Laboratories, Inc. | Digital assays for mutation detection |
US20150191707A1 (en) | 2013-11-14 | 2015-07-09 | Integrated Dna Technologies, Inc. | Dna polymerase mutants having enhanced template discrimination activity |
US11060149B2 (en) | 2014-06-18 | 2021-07-13 | Clear Gene, Inc. | Methods, compositions, and devices for rapid analysis of biological markers |
AU2015362617B2 (en) | 2014-12-15 | 2022-03-03 | Cepheid | Exponential base-greater-than-2 nucleic acid amplification |
EP3389481A4 (en) | 2015-12-18 | 2019-05-22 | Clear Gene, Inc. | Methods, compositions, kits and devices for rapid analysis of biological markers |
WO2019040788A1 (en) | 2017-08-24 | 2019-02-28 | Takara Bio Usa, Inc. | Methods of producing nucleic acids using oligonucleotides modified by a stimulus |
CN113227398A (en) * | 2018-10-29 | 2021-08-06 | 塞弗德公司 | Exponential-base-3 nucleic acid amplification with nested overlapping primers at reduced amplification time |
CN114467027A (en) * | 2019-05-20 | 2022-05-10 | 因斯利克萨公司 | Light-triggered nucleic acid constructs and methods for molecular detection |
US20220162687A1 (en) * | 2019-06-06 | 2022-05-26 | The Regents Of The University Of Colorado, A Body Corporate | Novel systems, methods and compositions for the direct synthesis of sticky ended polynucleotides |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5310652A (en) * | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
US5683896A (en) * | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
GB8920097D0 (en) * | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
US5422252A (en) * | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
US6027923A (en) * | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
US6335184B1 (en) * | 1993-07-23 | 2002-01-01 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
JPH09510351A (en) * | 1994-03-16 | 1997-10-21 | ジェン−プローブ・インコーポレイテッド | Isothermal strand displacement nucleic acid amplification method |
FR2737223B1 (en) * | 1995-07-24 | 1997-09-12 | Bio Merieux | METHOD OF AMPLIFYING NUCLEIC ACID SEQUENCES BY MOVEMENT USING CHIMERIC PRIMERS |
DE69612013T2 (en) * | 1995-11-21 | 2001-08-02 | Yale University, New Haven | UNIMOLECULAR SEGMENT AMPLIFICATION AND DETERMINATION |
US6124120A (en) * | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
IL148091A0 (en) * | 1999-09-13 | 2002-09-12 | Nugen Technologies Inc | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
CN1592792B (en) * | 2000-05-19 | 2010-12-01 | 伊拉根生物科学公司 | Materials and methods for detection of nucleic acids |
-
2003
- 2003-02-28 WO PCT/US2003/006293 patent/WO2003074724A2/en not_active Application Discontinuation
- 2003-02-28 DE DE60323611T patent/DE60323611D1/en not_active Expired - Lifetime
- 2003-02-28 AT AT03711339T patent/ATE408711T1/en not_active IP Right Cessation
- 2003-02-28 CA CA2476564A patent/CA2476564C/en not_active Expired - Fee Related
- 2003-02-28 AU AU2003213652A patent/AU2003213652A1/en not_active Abandoned
- 2003-02-28 US US10/377,168 patent/US7112406B2/en not_active Expired - Lifetime
- 2003-02-28 EP EP03711339A patent/EP1481088B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO2003074724A2 (en) | 2003-09-12 |
DE60323611D1 (en) | 2008-10-30 |
CA2476564C (en) | 2012-05-15 |
EP1481088B1 (en) | 2008-09-17 |
EP1481088A4 (en) | 2006-08-23 |
CA2476564A1 (en) | 2003-09-12 |
WO2003074724A3 (en) | 2004-03-25 |
US20040248095A1 (en) | 2004-12-09 |
AU2003213652A1 (en) | 2003-09-16 |
US7112406B2 (en) | 2006-09-26 |
EP1481088A2 (en) | 2004-12-01 |
AU2003213652A8 (en) | 2003-09-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties | ||
REN | Ceased due to non-payment of the annual fee |