ATE370158T1 - METHOD FOR THE ELECTROPHORETIC SEPARATION OF MEMBRANE PROTEINS - Google Patents

METHOD FOR THE ELECTROPHORETIC SEPARATION OF MEMBRANE PROTEINS

Info

Publication number
ATE370158T1
ATE370158T1 AT01957818T AT01957818T ATE370158T1 AT E370158 T1 ATE370158 T1 AT E370158T1 AT 01957818 T AT01957818 T AT 01957818T AT 01957818 T AT01957818 T AT 01957818T AT E370158 T1 ATE370158 T1 AT E370158T1
Authority
AT
Austria
Prior art keywords
membrane
proteins
membrane proteins
separated
electrophoretically
Prior art date
Application number
AT01957818T
Other languages
German (de)
Inventor
Thomas Bayerl
Erich Sackmann
Original Assignee
Nimbus Biotechnologie Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nimbus Biotechnologie Gmbh filed Critical Nimbus Biotechnologie Gmbh
Application granted granted Critical
Publication of ATE370158T1 publication Critical patent/ATE370158T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • C07K1/285—Isoelectric focusing multi dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The one and two-dimensional separation of proteins extending through the membrane (membrane proteins) is a prerequisite for a complete analysis of the proteome of a functionally intact cell organelle. A substantial drawback of prior art methods for separating is that their applicability is limited to peripheral water-soluble proteins. The aim of the novel method is to electrophoretically separate membrane proteins in one or two dimensions. To this end, membranes from cell organelles, whose membrane protein stock should be separated, are placed on planar supports whose surface was modified using suitable methods in such a manner that the membrane proteins maintain their ability to laterally diffuse in the membrane plane after being placed on the support. By subsequently applying electrical fields, the proteins can be electrophoretically displaced in the membrane plane and, as a result, can be separated in one or two dimensions according to their different charge characteristics.
AT01957818T 2000-06-03 2001-06-01 METHOD FOR THE ELECTROPHORETIC SEPARATION OF MEMBRANE PROTEINS ATE370158T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10027705A DE10027705A1 (en) 2000-06-03 2000-06-03 Separation of membrane proteins, useful for proteosome analysis, by attaching membranes to carrier then performing electrophoretic migration

Publications (1)

Publication Number Publication Date
ATE370158T1 true ATE370158T1 (en) 2007-09-15

Family

ID=7644690

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01957818T ATE370158T1 (en) 2000-06-03 2001-06-01 METHOD FOR THE ELECTROPHORETIC SEPARATION OF MEMBRANE PROTEINS

Country Status (7)

Country Link
US (1) US7175747B2 (en)
EP (1) EP1406919B1 (en)
AT (1) ATE370158T1 (en)
AU (2) AU2001279639B2 (en)
CA (1) CA2411684A1 (en)
DE (2) DE10027705A1 (en)
WO (1) WO2001094421A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10125713A1 (en) * 2001-05-21 2002-11-28 Nimbus Biotechnologie Gmbh Preparing immobilized lipid double layers, useful e.g. for investigating membrane proteins, comprises modifying carrier surface to fix orientation of the two layers
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
WO2005072854A1 (en) 2004-02-02 2005-08-11 The University Of British Columbia Scodaphoresis and methods and apparatus for moving and concentrating particles
US8518228B2 (en) 2011-05-20 2013-08-27 The University Of British Columbia Systems and methods for enhanced SCODA
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
EP1621877A1 (en) * 2004-07-30 2006-02-01 Julius-Maximilians-Universität Würzburg Method for contacting electrode-combs
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
US7795951B2 (en) * 2007-11-30 2010-09-14 Freescale Semiconductor, Inc. High-dynamic range low ripple voltage multiplier
CA2713313A1 (en) 2008-02-01 2009-08-06 The University Of British Columbia Methods and apparatus for particle introduction and recovery
US8877028B2 (en) 2009-04-21 2014-11-04 The University Of British Columbia System and methods for detection of particles
WO2013166444A2 (en) 2012-05-04 2013-11-07 Boreal Genomics Corp. Biomarker analysis using scodaphoresis
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
CN104655769B (en) * 2014-12-19 2017-01-04 山东省计量科学研究院 A kind of method using liquid chromatograph bilayer to fix phase
WO2016185284A1 (en) 2015-05-20 2016-11-24 Boreal Genomics, Inc. Method for isolating target nucleic acid using heteroduplex binding proteins
DE102017126732A1 (en) * 2017-11-14 2019-05-16 Endress+Hauser Conducta Gmbh+Co. Kg Sensor diaphragm, sensor, a measuring device comprising the sensor and use of the sensor
CA3159566A1 (en) 2019-10-29 2021-05-06 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6136296A (en) * 1984-07-30 1986-02-20 Lion Corp Separation and purification method of biomembrane protein
AU6410090A (en) * 1989-09-19 1991-04-18 Cell Technology, Inc. Free electrophoresis method for isolating ribosomes and natural membrane vesicles
US20010025791A1 (en) * 1999-12-15 2001-10-04 Landau Ehud M. Methods and devices for separation of biological molecules

Also Published As

Publication number Publication date
DE50112881D1 (en) 2007-09-27
AU7963901A (en) 2001-12-17
US7175747B2 (en) 2007-02-13
WO2001094421A3 (en) 2002-05-10
DE10027705A1 (en) 2001-12-13
CA2411684A1 (en) 2001-12-13
WO2001094421A2 (en) 2001-12-13
US20030159933A1 (en) 2003-08-28
AU2001279639B2 (en) 2006-09-21
EP1406919B1 (en) 2007-08-15
EP1406919A2 (en) 2004-04-14

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Legal Events

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