ATE323775T1 - Adenovirus-mutanten mit deletiertem proteasegen, komplementierende zelllinien und entsprechende vektoren für den gentransfer - Google Patents
Adenovirus-mutanten mit deletiertem proteasegen, komplementierende zelllinien und entsprechende vektoren für den gentransferInfo
- Publication number
- ATE323775T1 ATE323775T1 AT00906111T AT00906111T ATE323775T1 AT E323775 T1 ATE323775 T1 AT E323775T1 AT 00906111 T AT00906111 T AT 00906111T AT 00906111 T AT00906111 T AT 00906111T AT E323775 T1 ATE323775 T1 AT E323775T1
- Authority
- AT
- Austria
- Prior art keywords
- protease
- gene
- deleted
- positive selection
- region
- Prior art date
Links
- 241000701161 unidentified adenovirus Species 0.000 title abstract 6
- 108091005804 Peptidases Proteins 0.000 title abstract 5
- 239000013598 vector Substances 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 108091029865 Exogenous DNA Proteins 0.000 abstract 2
- 239000013605 shuttle vector Substances 0.000 abstract 2
- 230000003612 virological effect Effects 0.000 abstract 2
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000005215 recombination Methods 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
- C12N2710/10352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
- C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/258,209 US6291226B1 (en) | 1999-02-25 | 1999-02-25 | Adenovirus mutants with deleted protease gene |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE323775T1 true ATE323775T1 (de) | 2006-05-15 |
Family
ID=22979566
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT00906111T ATE323775T1 (de) | 1999-02-25 | 2000-02-24 | Adenovirus-mutanten mit deletiertem proteasegen, komplementierende zelllinien und entsprechende vektoren für den gentransfer |
Country Status (6)
Country | Link |
---|---|
US (2) | US6291226B1 (de) |
EP (2) | EP1073760B1 (de) |
AT (1) | ATE323775T1 (de) |
CA (1) | CA2322222C (de) |
DE (1) | DE60027364T2 (de) |
WO (1) | WO2000050619A1 (de) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6642052B2 (en) * | 1999-02-25 | 2003-11-04 | National Research Council Of Canada | Efficient generation of adenovirus-based libraries by positive selection of adenoviral recombinants through ectopic expression of the adenovirus protease |
CA2432676A1 (en) * | 2002-07-31 | 2004-01-31 | Robert Menard | Development of an in vivo functional assay for proteases |
US7935788B2 (en) * | 2004-10-04 | 2011-05-03 | National Research Council Of Canada | Reverse cumate repressor mutant |
WO2006037215A1 (en) | 2004-10-04 | 2006-04-13 | National Research Council Of Canada | Expression system, components thereof and methods of use |
SI2147105T1 (sl) | 2007-05-02 | 2013-08-30 | Merial Limited | DNA-plazmidi z izboljšano ekspresijo in stabilnostjo |
US11414677B2 (en) | 2017-10-27 | 2022-08-16 | Massachusetts Institute Of Technology | Methods and compositions for performing continuous directed evolution |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5543264A (en) | 1990-06-29 | 1996-08-06 | Associated Universities, Inc. | Co-factor activated recombinant adenovirus proteinases |
US5846945A (en) | 1993-02-16 | 1998-12-08 | Onyx Pharmaceuticals, Inc. | Cytopathic viruses for therapy and prophylaxis of neoplasia |
US5866755A (en) | 1993-06-14 | 1999-02-02 | Basf Aktiengellschaft | Animals transgenic for a tetracycline-regulated transcriptional inhibitor |
HU216871B (hu) * | 1993-07-13 | 1999-09-28 | Rhone-Poulenc Rorer S.A. | Defektív adenovírusvektorok és génterápiai alkalmazásuk |
WO1996002697A1 (en) * | 1994-07-14 | 1996-02-01 | Union Camp Patent Holding, Inc. | Improved bleaching of high consistency lignocellulosic pulp |
US5972666A (en) | 1996-07-26 | 1999-10-26 | G. D. Searle & Co. | Assembly-deficient herpesvirus vaccine |
US5994134A (en) * | 1998-05-04 | 1999-11-30 | Canji, Inc. | Viral production process |
-
1999
- 1999-02-25 US US09/258,209 patent/US6291226B1/en not_active Expired - Lifetime
-
2000
- 2000-02-24 AT AT00906111T patent/ATE323775T1/de not_active IP Right Cessation
- 2000-02-24 CA CA2322222A patent/CA2322222C/en not_active Expired - Lifetime
- 2000-02-24 WO PCT/CA2000/000197 patent/WO2000050619A1/en active IP Right Grant
- 2000-02-24 DE DE60027364T patent/DE60027364T2/de not_active Expired - Fee Related
- 2000-02-24 EP EP00906111A patent/EP1073760B1/de not_active Expired - Lifetime
-
2001
- 2001-03-30 US US09/820,824 patent/US6620618B2/en not_active Expired - Lifetime
-
2002
- 2002-04-30 EP EP02253042A patent/EP1291434A3/de not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
US6620618B2 (en) | 2003-09-16 |
DE60027364D1 (de) | 2006-05-24 |
CA2322222A1 (en) | 2000-08-31 |
CA2322222C (en) | 2010-05-18 |
EP1291434A2 (de) | 2003-03-12 |
EP1073760A1 (de) | 2001-02-07 |
US6291226B1 (en) | 2001-09-18 |
DE60027364T2 (de) | 2007-05-10 |
EP1291434A3 (de) | 2003-12-03 |
WO2000050619A1 (en) | 2000-08-31 |
EP1073760B1 (de) | 2006-04-19 |
US20010026938A1 (en) | 2001-10-04 |
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by RU486-Induced | ADENOVIRUS VECTORS: VECTOR TECHNOLOGY |
Legal Events
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |