ATE317014T1 - Bifunktionelle enzyme zur biopolymerherstellung - Google Patents

Bifunktionelle enzyme zur biopolymerherstellung

Info

Publication number
ATE317014T1
ATE317014T1 AT99937729T AT99937729T ATE317014T1 AT E317014 T1 ATE317014 T1 AT E317014T1 AT 99937729 T AT99937729 T AT 99937729T AT 99937729 T AT99937729 T AT 99937729T AT E317014 T1 ATE317014 T1 AT E317014T1
Authority
AT
Austria
Prior art keywords
enzymes
pathway
flux
optimize
reaction
Prior art date
Application number
AT99937729T
Other languages
English (en)
Inventor
Oliver P Peoples
Lara Madison
Gjalt W Huisman
Original Assignee
Metabolix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolix Inc filed Critical Metabolix Inc
Application granted granted Critical
Publication of ATE317014T1 publication Critical patent/ATE317014T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT99937729T 1998-07-30 1999-07-30 Bifunktionelle enzyme zur biopolymerherstellung ATE317014T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9467498P 1998-07-30 1998-07-30

Publications (1)

Publication Number Publication Date
ATE317014T1 true ATE317014T1 (de) 2006-02-15

Family

ID=22246500

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99937729T ATE317014T1 (de) 1998-07-30 1999-07-30 Bifunktionelle enzyme zur biopolymerherstellung

Country Status (9)

Country Link
US (3) US20020173019A1 (de)
EP (1) EP1100928B1 (de)
JP (1) JP2002521060A (de)
AT (1) ATE317014T1 (de)
AU (1) AU768244B2 (de)
CA (1) CA2337099C (de)
DE (1) DE69929700T2 (de)
MX (1) MXPA01001049A (de)
WO (1) WO2000006747A2 (de)

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US20020132308A1 (en) * 2000-08-24 2002-09-19 Mpep @ Page 300-M Novel constructs and their use in metabolic pathway engineering
DE10240035A1 (de) * 2002-08-30 2004-03-11 Rehm, Bernd H.A., PD Dr.rer.nat. Biogene Polyester-Partikel definierter Größe mit funktionalisierten Oberflächen:Herstellungsverfahren und pharmazeutische Zubereitungen, in denen diese enthalten sind
JP2008532553A (ja) 2005-03-16 2008-08-21 メタボリックス， インコーポレイテッド 生合成経路の化学的に誘導可能な発現
EP1929010B1 (de) * 2005-09-27 2013-12-18 PolyBatics Limited Verwendung von polymerteilchen
TWI534265B (zh) * 2008-09-15 2016-05-21 農業維多利亞服務有限公司 用於操縱植物之光合細胞中果聚糖生合成之方法、基因建構體及基因轉殖植物細胞
WO2010057022A1 (en) * 2008-11-14 2010-05-20 Genomatica, Inc. Microorganisms for the production of methyl ethyl ketone and 2-butanol
US20130071892A1 (en) * 2010-02-26 2013-03-21 Tokyo Institute Of Technology PROCESS FOR PRODUCTION OF POLYHYDROXYALKANOIC ACID USING GENETICALLY MODIFIED MICROORGANISM HAVING ENOYL-CoA HYDRATASE GENE INTRODUCED THEREIN
US20140073022A1 (en) * 2012-09-10 2014-03-13 Wisconsin Alumni Research Foundation Production of polyhydroxyalkanoates with a defined composition from an unrelated carbon source
CN110366594B (zh) * 2016-12-16 2023-12-15 丹尼斯科美国公司 双功能磷酸转酮酶-磷酸转乙酰酶融合多肽

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US5034322A (en) * 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
US5352605A (en) * 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
US5231019A (en) * 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US5668298A (en) * 1984-12-24 1997-09-16 Eli Lilly And Company Selectable marker for development of vectors and transformation systems in plants
US5420034A (en) * 1986-07-31 1995-05-30 Calgene, Inc. Seed-specific transcriptional regulation
US5188958A (en) * 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5024944A (en) * 1986-08-04 1991-06-18 Lubrizol Genetics, Inc. Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
US5276268A (en) * 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5268463A (en) * 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5004863B2 (en) * 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5030572A (en) * 1987-04-01 1991-07-09 Lubrizol Genetics, Inc. Sunflower regeneration from cotyledons
US5250430A (en) * 1987-06-29 1993-10-05 Massachusetts Institute Of Technology Polyhydroxyalkanoate polymerase
US5245023A (en) * 1987-06-29 1993-09-14 Massachusetts Institute Of Technology Method for producing novel polyester biopolymers
US5892019A (en) * 1987-07-15 1999-04-06 The United States Of America, As Represented By The Department Of Health And Human Services Production of a single-gene-encoded immunoglobulin
ATE105585T1 (de) * 1987-12-21 1994-05-15 Univ Toledo Transformation von keimenden pflanzensamen mit hilfe von agrobacterium.
US5416011A (en) * 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
EP0388186A1 (de) * 1989-03-17 1990-09-19 E.I. Du Pont De Nemours And Company Externe Regulierung der Genexpression
US5629183A (en) * 1989-05-08 1997-05-13 The United States Of America As Represented By The Secretary Of Agriculture Plant transformation by gene transfer into pollen
US5302523A (en) * 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
WO1991000917A1 (en) * 1989-07-10 1991-01-24 Massachusetts Institute Of Technology Method for producing novel polyester biopolymers
US5322783A (en) * 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5484956A (en) * 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5384253A (en) * 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
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US5610041A (en) * 1991-07-19 1997-03-11 Board Of Trustees Operating Michigan State University Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants
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Also Published As

Publication number Publication date
DE69929700T2 (de) 2006-08-31
US20020173019A1 (en) 2002-11-21
CA2337099A1 (en) 2000-02-10
WO2000006747A3 (en) 2000-03-23
JP2002521060A (ja) 2002-07-16
EP1100928A2 (de) 2001-05-23
AU768244B2 (en) 2003-12-04
US20060183209A1 (en) 2006-08-17
CA2337099C (en) 2005-01-11
MXPA01001049A (es) 2002-08-20
EP1100928B1 (de) 2006-02-01
US20080233629A1 (en) 2008-09-25
WO2000006747A2 (en) 2000-02-10
AU5250299A (en) 2000-02-21
DE69929700D1 (de) 2006-04-13

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