ATE236403T1 - Verfahren zur bevorzugten markierung von phycobiliprotein mittels aminreaktivem farbstoff zur verwendung in einem mehrfarbentest - Google Patents
Verfahren zur bevorzugten markierung von phycobiliprotein mittels aminreaktivem farbstoff zur verwendung in einem mehrfarbentestInfo
- Publication number
- ATE236403T1 ATE236403T1 AT93921202T AT93921202T ATE236403T1 AT E236403 T1 ATE236403 T1 AT E236403T1 AT 93921202 T AT93921202 T AT 93921202T AT 93921202 T AT93921202 T AT 93921202T AT E236403 T1 ATE236403 T1 AT E236403T1
- Authority
- AT
- Austria
- Prior art keywords
- phycobiliprotein
- reactive dye
- conjugates
- amine
- amine reactive
- Prior art date
Links
- 108060006184 phycobiliprotein Proteins 0.000 title abstract 4
- 239000000985 reactive dye Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 238000002372 labelling Methods 0.000 title 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 2
- VDABVNMGKGUPEY-UHFFFAOYSA-N 6-carboxyfluorescein succinimidyl ester Chemical compound C=1C(O)=CC=C2C=1OC1=CC(O)=CC=C1C2(C1=C2)OC(=O)C1=CC=C2C(=O)ON1C(=O)CCC1=O VDABVNMGKGUPEY-UHFFFAOYSA-N 0.000 abstract 1
- 108010004729 Phycoerythrin Proteins 0.000 abstract 1
- 108010004469 allophycocyanin Proteins 0.000 abstract 1
- 238000003271 compound fluorescence assay Methods 0.000 abstract 1
- -1 for example Proteins 0.000 abstract 1
- 238000010791 quenching Methods 0.000 abstract 1
- 230000000171 quenching effect Effects 0.000 abstract 1
- 230000008707 rearrangement Effects 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/940,026 US5272257A (en) | 1990-05-21 | 1992-09-03 | Method of preferential labelling of a phycobiliprotein with an amine-reactive dye for use in a multiple color assay and product for such use |
| US99086192A | 1992-12-11 | 1992-12-11 | |
| PCT/US1993/008046 WO1994005701A1 (en) | 1992-09-03 | 1993-08-27 | Method of preferential labelling of phycobiliprotein with amine-reactive dye for use in a multiple color assay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE236403T1 true ATE236403T1 (de) | 2003-04-15 |
Family
ID=27130128
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT93921202T ATE236403T1 (de) | 1992-09-03 | 1993-08-27 | Verfahren zur bevorzugten markierung von phycobiliprotein mittels aminreaktivem farbstoff zur verwendung in einem mehrfarbentest |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0673392B1 (de) |
| JP (1) | JPH08501295A (de) |
| AT (1) | ATE236403T1 (de) |
| AU (1) | AU685062B2 (de) |
| CA (1) | CA2143757A1 (de) |
| DE (1) | DE69332828D1 (de) |
| WO (1) | WO1994005701A1 (de) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000017650A1 (en) * | 1998-09-23 | 2000-03-30 | Molecular Probes, Inc. | Energy transfer compositions comprising phycobiliproteins |
| US20100330703A1 (en) * | 2009-06-24 | 2010-12-30 | Seventh Sense Biosystems, Inc. | Assays involving colorimetric and other signaling |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4542104A (en) * | 1983-04-06 | 1985-09-17 | The Board Of Trustees Of The Leland Stanford Jr. Univ. | Phycobiliprotein fluorescent conjugates |
| US4666862A (en) * | 1984-08-14 | 1987-05-19 | Ortho Diagnostic Systems Inc. | Fluorescent energy transfer with phycobiliproteins |
| US5171846A (en) * | 1990-05-21 | 1992-12-15 | Coulter Corporation | Method of preferential labelling of a phycobiliprotein with a second dye for use in a multiple color assay and product for such use |
-
1993
- 1993-08-27 JP JP6507312A patent/JPH08501295A/ja active Pending
- 1993-08-27 AT AT93921202T patent/ATE236403T1/de not_active IP Right Cessation
- 1993-08-27 DE DE69332828T patent/DE69332828D1/de not_active Expired - Lifetime
- 1993-08-27 CA CA002143757A patent/CA2143757A1/en not_active Abandoned
- 1993-08-27 EP EP93921202A patent/EP0673392B1/de not_active Expired - Lifetime
- 1993-08-27 AU AU48385/93A patent/AU685062B2/en not_active Ceased
- 1993-08-27 WO PCT/US1993/008046 patent/WO1994005701A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP0673392B1 (de) | 2003-04-02 |
| AU4838593A (en) | 1994-03-29 |
| WO1994005701A1 (en) | 1994-03-17 |
| CA2143757A1 (en) | 1994-03-17 |
| JPH08501295A (ja) | 1996-02-13 |
| DE69332828D1 (de) | 2003-05-08 |
| EP0673392A4 (de) | 1997-10-15 |
| EP0673392A1 (de) | 1995-09-27 |
| AU685062B2 (en) | 1998-01-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |