ATE214095T1 - USE OF EXO-NUCLEOTIDE SAMPLE IN GENE CLONING - Google Patents

USE OF EXO-NUCLEOTIDE SAMPLE IN GENE CLONING

Info

Publication number
ATE214095T1
ATE214095T1 AT92914436T AT92914436T ATE214095T1 AT E214095 T1 ATE214095 T1 AT E214095T1 AT 92914436 T AT92914436 T AT 92914436T AT 92914436 T AT92914436 T AT 92914436T AT E214095 T1 ATE214095 T1 AT E214095T1
Authority
AT
Austria
Prior art keywords
gene cloning
exo
nucleotide sample
provides methods
present
Prior art date
Application number
AT92914436T
Other languages
German (de)
Inventor
Mark S Berninger
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE214095T1 publication Critical patent/ATE214095T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides methods for manipulating recombinant DNA in gene cloning and expression. More specifically , the invention provides methods capable of altering a nucleic acid sequence present at the termini of a target sequence.
AT92914436T 1991-06-14 1992-06-12 USE OF EXO-NUCLEOTIDE SAMPLE IN GENE CLONING ATE214095T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/715,623 US5137814A (en) 1991-06-14 1991-06-14 Use of exo-sample nucleotides in gene cloning
PCT/US1992/004922 WO1992022649A1 (en) 1991-06-14 1992-06-12 Use of exo-sample nucleotides in gene cloning

Publications (1)

Publication Number Publication Date
ATE214095T1 true ATE214095T1 (en) 2002-03-15

Family

ID=24874814

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92914436T ATE214095T1 (en) 1991-06-14 1992-06-12 USE OF EXO-NUCLEOTIDE SAMPLE IN GENE CLONING

Country Status (9)

Country Link
US (1) US5137814A (en)
EP (2) EP1156108A3 (en)
JP (1) JP3395905B2 (en)
AT (1) ATE214095T1 (en)
CA (1) CA2088766A1 (en)
DE (1) DE69232457T2 (en)
DK (1) DK0544894T3 (en)
ES (1) ES2173867T3 (en)
WO (1) WO1992022649A1 (en)

Families Citing this family (49)

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US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5229283A (en) * 1991-06-14 1993-07-20 Life Technologies, Inc. Use of exo-sample nucleotides in gene cloning
WO1993018175A1 (en) * 1992-03-10 1993-09-16 Life Technologies, Inc. Udg-facilitated mutagenesis
US5426039A (en) * 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
DE69411753T2 (en) * 1993-10-28 1999-02-04 Life Technologies Inc NUCLEIC ACID LENGTH STANDARD FOR MEASURING THE MASS
US6680378B1 (en) * 1993-10-28 2004-01-20 Invitrogen Corporation Nucleic acid marker ladder for estimating mass
US6495318B2 (en) * 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
EP0915903A4 (en) * 1996-06-17 2002-09-04 Biodynamics Associates Method and kits for preparing multicomponent nucleic acid constructs
IL120339A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6365731B1 (en) 1997-08-06 2002-04-02 Ambion, Inc. Stripping nucleic acids with iodine and sodium thiosulfate
US6815185B2 (en) * 1997-11-17 2004-11-09 Robert D. Klein Methods of creating constructs useful for introducing sequences into embryonic stem cells
JP2003500070A (en) * 1999-05-21 2003-01-07 インビトロゲン・コーポレーション Compositions and methods for labeling nucleic acid molecules
AU5882000A (en) 1999-06-22 2001-01-09 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6998273B1 (en) * 2000-02-09 2006-02-14 A-Fem Medical Corporation Collection device for lateral flow chromatography
US6660475B2 (en) 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
AU2003239129A1 (en) * 2002-04-12 2003-10-27 New England Biolabs, Inc. Methods and compositions for dna manipulation
US7273730B2 (en) * 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
US7531306B2 (en) * 2003-11-12 2009-05-12 Geneohm Sciences, Inc. Nucleic acid hybridization methods
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
US9505846B2 (en) 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
US7754944B2 (en) * 2006-04-03 2010-07-13 Monsanto Technology Llc Polynucleotide constructs
CN101979592B (en) * 2010-10-27 2012-05-09 上海交通大学 Nucleotide sequence of acetolactate synthase gene of anabaena azotica ley
US20150037787A1 (en) * 2013-07-31 2015-02-05 International Business Machines Corporation Polynucleotide configuration for reliable electrical and optical sensing
KR102291045B1 (en) 2013-08-05 2021-08-19 트위스트 바이오사이언스 코포레이션 De novo synthesized gene libraries
WO2016022557A1 (en) * 2014-08-05 2016-02-11 Twist Bioscience Corporation Cell free cloning of nucleic acids
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2016183100A1 (en) * 2015-05-11 2016-11-17 Twist Bioscience Corporation Compositions and methods for nucleic acid amplification
US10844373B2 (en) 2015-09-18 2020-11-24 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (en) 2015-09-22 2018-10-23 特韦斯特生物科学公司 Flexible substrates for nucleic acid synthesis
CN115920796A (en) 2015-12-01 2023-04-07 特韦斯特生物科学公司 Functionalized surfaces and preparation thereof
GB2568444A (en) 2016-08-22 2019-05-15 Twist Bioscience Corp De novo synthesized nucleic acid libraries
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
CA3047128A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
JP2020536504A (en) 2017-09-11 2020-12-17 ツイスト バイオサイエンス コーポレーション GPCR-coupled protein and its synthesis
WO2019079769A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
AU2019205269A1 (en) 2018-01-04 2020-07-30 Twist Bioscience Corporation DNA-based digital information storage
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
SG11202109283UA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for antibody optimization
CN113766930A (en) 2019-02-26 2021-12-07 特韦斯特生物科学公司 Variant nucleic acid libraries of GLP1 receptors
EP3987019A4 (en) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873192A (en) * 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
EP0397463A3 (en) * 1989-05-10 1991-03-06 Eastman Kodak Company Construction of synthetic double-stranded dna sequences
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions

Also Published As

Publication number Publication date
EP0544894A1 (en) 1993-06-09
WO1992022649A1 (en) 1992-12-23
DK0544894T3 (en) 2002-04-22
ES2173867T3 (en) 2002-11-01
EP1156108A3 (en) 2001-12-12
CA2088766A1 (en) 1992-12-15
DE69232457T2 (en) 2002-10-10
EP0544894B1 (en) 2002-03-06
JP3395905B2 (en) 2003-04-14
DE69232457D1 (en) 2002-04-11
JPH06500702A (en) 1994-01-27
EP0544894A4 (en) 1994-10-19
EP1156108A2 (en) 2001-11-21
US5137814A (en) 1992-08-11

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