ATE213832T1 - DETECTION SYSTEM WITH FLUOROMETER - Google Patents

DETECTION SYSTEM WITH FLUOROMETER

Info

Publication number
ATE213832T1
ATE213832T1 AT93908431T AT93908431T ATE213832T1 AT E213832 T1 ATE213832 T1 AT E213832T1 AT 93908431 T AT93908431 T AT 93908431T AT 93908431 T AT93908431 T AT 93908431T AT E213832 T1 ATE213832 T1 AT E213832T1
Authority
AT
Austria
Prior art keywords
time
detection
histogram
compile
photon
Prior art date
Application number
AT93908431T
Other languages
German (de)
Inventor
Robert Marlin Studholme
David Arthur Blau
Original Assignee
Hyperion Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hyperion Inc filed Critical Hyperion Inc
Application granted granted Critical
Publication of ATE213832T1 publication Critical patent/ATE213832T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging

Abstract

An improved fluorescence detection system is provided which utilizes a relatively high powered, relatively high repetition rate light source with high speed detection electronics to increase system sensitivity and accuracy. In the preferred embodiments, a laser diode is the light source. In one embodiment, the position of a time window is varied to compile a decay profile of a fluorophore. In another embodiment, the time to detection of a photon is used to compile the decay profile. In one aspect of this invention, a histogram of the fluorescence decay is generated by determining a preliminary histogram of the shape and multiplying it by the ratio of the total number of events divided by the number of events comprising the preliminary histogram. In another aspect of this invention, the time of detection after excitation of the photon is started from a random time, such as after a preceding event is detected and the data stored.
AT93908431T 1992-03-23 1993-03-22 DETECTION SYSTEM WITH FLUOROMETER ATE213832T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/855,238 US5323008A (en) 1992-03-23 1992-03-23 Fluorometer detection system
PCT/US1993/002540 WO1993019358A1 (en) 1992-03-23 1993-03-22 Fluorometer detection system

Publications (1)

Publication Number Publication Date
ATE213832T1 true ATE213832T1 (en) 2002-03-15

Family

ID=25320715

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93908431T ATE213832T1 (en) 1992-03-23 1993-03-22 DETECTION SYSTEM WITH FLUOROMETER

Country Status (8)

Country Link
US (1) US5323008A (en)
EP (1) EP0632887B1 (en)
JP (2) JPH07505467A (en)
AT (1) ATE213832T1 (en)
CA (2) CA2538701C (en)
DE (1) DE69331629T2 (en)
ES (1) ES2173093T3 (en)
WO (1) WO1993019358A1 (en)

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Also Published As

Publication number Publication date
CA2132707A1 (en) 1993-09-30
CA2538701C (en) 2006-11-28
EP0632887A1 (en) 1995-01-11
US5323008A (en) 1994-06-21
ES2173093T3 (en) 2002-10-16
WO1993019358A1 (en) 1993-09-30
DE69331629T2 (en) 2002-08-14
JPH07505467A (en) 1995-06-15
DE69331629D1 (en) 2002-04-04
CA2538701A1 (en) 1993-09-30
CA2132707C (en) 2006-06-13
EP0632887B1 (en) 2002-02-27
JP2002323448A (en) 2002-11-08
EP0632887A4 (en) 1995-11-22

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