ATE140798T1 - LIPID-DEPENDENT DIAGNOSTIC ANALYSIS METHODS - Google Patents

LIPID-DEPENDENT DIAGNOSTIC ANALYSIS METHODS

Info

Publication number
ATE140798T1
ATE140798T1 AT91902056T AT91902056T ATE140798T1 AT E140798 T1 ATE140798 T1 AT E140798T1 AT 91902056 T AT91902056 T AT 91902056T AT 91902056 T AT91902056 T AT 91902056T AT E140798 T1 ATE140798 T1 AT E140798T1
Authority
AT
Austria
Prior art keywords
assay
phospholipid
suspension
sample
lipid
Prior art date
Application number
AT91902056T
Other languages
German (de)
Inventor
Andrew S Janoff
Joyce Rauch
Original Assignee
Liposome Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Liposome Co Inc filed Critical Liposome Co Inc
Application granted granted Critical
Publication of ATE140798T1 publication Critical patent/ATE140798T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/04—Phospholipids, i.e. phosphoglycerides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Rehabilitation Therapy (AREA)
  • Rheumatology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

For use in a lipid-dependent diagnostic assay, a stable aqueous suspension of a phospholipid which normally has a hexagonal (HII) organization when dispersed in an aqueous medium without detergent, the suspension containing the phospholipid, a detergent, and an aqueous phase. In the stable suspension, the phospholipid remains in suspension at a temperature of 25 DEG C for at least one hour. The suspension is suitable for providing the phospholipid to an assay for lupus anticoagulants which includes the step of pre-incubating a test sample with the phospholipid. In a further aspect of the invention an assay is provided for use in determining whether a patient has a lupus anticoagulant, the assay including the steps of: a) obtaining first and second samples of the patient's plasma; b) incubating the first sample with the stable aqueous phospholipid suspension of the present invention; c) performing a lipid-dependent diagnostic assay on both the first and second samples, the assay producing a positive reading when used to assay a sample which contains lupus anticoagulants; and d) comparing the results of the assays performed on the first and second samples, the presence of a normal result for the first sample and a positive result for the second sample being indicative of the patient having a lupus anticoagulant.
AT91902056T 1990-12-07 1990-12-28 LIPID-DEPENDENT DIAGNOSTIC ANALYSIS METHODS ATE140798T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US62334090A 1990-12-07 1990-12-07

Publications (1)

Publication Number Publication Date
ATE140798T1 true ATE140798T1 (en) 1996-08-15

Family

ID=24497709

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91902056T ATE140798T1 (en) 1990-12-07 1990-12-28 LIPID-DEPENDENT DIAGNOSTIC ANALYSIS METHODS

Country Status (10)

Country Link
EP (1) EP0561780B1 (en)
JP (1) JP2983630B2 (en)
AT (1) ATE140798T1 (en)
AU (1) AU648775B2 (en)
CA (1) CA2095928C (en)
DE (1) DE69027936T2 (en)
DK (1) DK0561780T3 (en)
ES (1) ES2090306T3 (en)
GR (1) GR3021412T3 (en)
WO (1) WO1992010586A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4229933A1 (en) * 1992-09-08 1994-03-10 Max Planck Gesellschaft Method for the determination of thrombosis-causing lupus anticoagulant antibodies
US5472852A (en) * 1993-09-15 1995-12-05 Oklahoma Medical Research Foundation Assay for detection of selective Protein C inhibition by patients
JP4377207B2 (en) * 2003-11-28 2009-12-02 シスメックス株式会社 Blood coagulation time measurement method and blood coagulation time measurement reagent

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4543339A (en) * 1982-03-16 1985-09-24 Oneill Christopher Early pregnancy detection by detecting enhanced blood platelet activation
US4698299A (en) * 1985-02-19 1987-10-06 The Liposome Company, Inc. Lipid-dependent diagnostic assays
US4666831A (en) * 1985-02-19 1987-05-19 The Liposome Company, Inc. Lipid-dependent diagnostic assays
EP0303515B1 (en) * 1987-08-13 1992-11-11 Synbiotics Corporation Improved amphipathic analyte assays using lipophilic surface
US4877741A (en) * 1988-10-24 1989-10-31 The United States Of America As Represented By The Secretary Of The Air Force Treatment of human plasma with brown recluse spider toxin to emulate a lupus anticoagulant

Also Published As

Publication number Publication date
AU648775B2 (en) 1994-05-05
DE69027936T2 (en) 1997-03-06
GR3021412T3 (en) 1997-01-31
AU7162091A (en) 1992-07-08
EP0561780A1 (en) 1993-09-29
CA2095928A1 (en) 1992-06-08
ES2090306T3 (en) 1996-10-16
CA2095928C (en) 1999-08-31
EP0561780B1 (en) 1996-07-24
JPH06503640A (en) 1994-04-21
EP0561780A4 (en) 1994-08-03
JP2983630B2 (en) 1999-11-29
DE69027936D1 (en) 1996-08-29
DK0561780T3 (en) 1996-12-09
WO1992010586A1 (en) 1992-06-25

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Legal Events

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UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee