ATE116376T1 - Kompetitives pcr zur quantifizierung von dns. - Google Patents
Kompetitives pcr zur quantifizierung von dns.Info
- Publication number
- ATE116376T1 ATE116376T1 AT91913251T AT91913251T ATE116376T1 AT E116376 T1 ATE116376 T1 AT E116376T1 AT 91913251 T AT91913251 T AT 91913251T AT 91913251 T AT91913251 T AT 91913251T AT E116376 T1 ATE116376 T1 AT E116376T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- amplified
- competitor
- amount
- target dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB909016163A GB9016163D0 (en) | 1990-07-24 | 1990-07-24 | Chemical method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE116376T1 true ATE116376T1 (de) | 1995-01-15 |
Family
ID=10679528
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT91913251T ATE116376T1 (de) | 1990-07-24 | 1991-07-23 | Kompetitives pcr zur quantifizierung von dns. |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0540585B1 (de) |
| JP (1) | JP3138471B2 (de) |
| AT (1) | ATE116376T1 (de) |
| AU (1) | AU648330B2 (de) |
| CA (1) | CA2087982A1 (de) |
| DE (1) | DE69106366T2 (de) |
| DK (1) | DK0540585T3 (de) |
| GB (1) | GB9016163D0 (de) |
| WO (1) | WO1992001812A1 (de) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2683827B1 (fr) * | 1991-11-15 | 1994-03-04 | Institut Nal Sante Recherc Medic | Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique. |
| ZA936015B (en) * | 1992-08-24 | 1994-03-10 | Akzo Nv | Elimination of false negatives in nuleic acid detection. |
| DE69430909T2 (de) * | 1993-03-19 | 2003-02-27 | Sequenom Inc | Dns-sequenzbestimmung durch massenspektrometrie auf dem weg des abbaus mit exonuklease |
| US5457027A (en) * | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
| GB9311386D0 (en) * | 1993-06-02 | 1993-07-21 | Pna Diagnostics As | Nucleic acid analogue assay procedures |
| US5837501A (en) * | 1993-07-09 | 1998-11-17 | Akzo Nobel N.V. | Nucleic acid quantitation by co-amplification of target with multiple internal controls |
| GB9314394D0 (en) * | 1993-07-12 | 1993-08-25 | Slater James H | A security device using an ultrasensitive microtrace for protecting materials,articles and items |
| US5547861A (en) * | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
| GB9519638D0 (en) * | 1995-09-26 | 1995-11-29 | Dynal As | Method |
| EP0929696A2 (de) * | 1996-09-09 | 1999-07-21 | Eppendorf Array Technologies (E.A.T.) | Verfahren und reagentiensatz zur erkennung und/oder quantifizierung von nukleinsäuren durch sandwich-hybridisierung an der festphase |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| JP2001147230A (ja) | 1999-11-19 | 2001-05-29 | Hitachi Software Eng Co Ltd | バイオチップ読取装置及び標識試薬 |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| US8858855B2 (en) | 2006-04-20 | 2014-10-14 | Boston Scientific Scimed, Inc. | High pressure balloon |
| US9180279B2 (en) | 2006-08-07 | 2015-11-10 | W. L. Gore & Associates, Inc. | Inflatable imbibed polymer devices |
| US8772046B2 (en) | 2007-02-06 | 2014-07-08 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
| US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
| US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
| US9492797B2 (en) | 2008-09-23 | 2016-11-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| US9598725B2 (en) | 2010-03-02 | 2017-03-21 | Bio-Rad Laboratories, Inc. | Emulsion chemistry for encapsulated droplets |
| US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
| EP2473618B1 (de) | 2009-09-02 | 2015-03-04 | Bio-Rad Laboratories, Inc. | System zum mischen von flüssigkeiten durch gerinnung mehrerer emulsionen |
| JP2011062119A (ja) | 2009-09-16 | 2011-03-31 | Seiko Epson Corp | 生体試料定量用チップ |
| JP6155419B2 (ja) | 2010-03-25 | 2017-07-05 | バイオ−ラッド・ラボラトリーズ・インコーポレーテッド | 検出用の液滴輸送システム |
| CA2767182C (en) | 2010-03-25 | 2020-03-24 | Bio-Rad Laboratories, Inc. | Droplet generation for droplet-based assays |
| DE202011110979U1 (de) | 2010-11-01 | 2017-12-04 | Bio-Rad Laboratories, Inc. | System zum Bilden von Emulsionen |
| CA2830443C (en) | 2011-03-18 | 2021-11-16 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
| CA2834291A1 (en) | 2011-04-25 | 2012-11-01 | Biorad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| WO2013155531A2 (en) | 2012-04-13 | 2013-10-17 | Bio-Rad Laboratories, Inc. | Sample holder with a well having a wicking promoter |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
-
1990
- 1990-07-24 GB GB909016163A patent/GB9016163D0/en active Pending
-
1991
- 1991-07-23 AU AU82386/91A patent/AU648330B2/en not_active Ceased
- 1991-07-23 DK DK91913251.4T patent/DK0540585T3/da active
- 1991-07-23 WO PCT/EP1991/001398 patent/WO1992001812A1/en active IP Right Grant
- 1991-07-23 CA CA002087982A patent/CA2087982A1/en not_active Abandoned
- 1991-07-23 AT AT91913251T patent/ATE116376T1/de not_active IP Right Cessation
- 1991-07-23 JP JP03512534A patent/JP3138471B2/ja not_active Expired - Fee Related
- 1991-07-23 DE DE69106366T patent/DE69106366T2/de not_active Expired - Fee Related
- 1991-07-23 EP EP91913251A patent/EP0540585B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69106366D1 (de) | 1995-02-09 |
| JPH05508545A (ja) | 1993-12-02 |
| DE69106366T2 (de) | 1995-05-11 |
| JP3138471B2 (ja) | 2001-02-26 |
| AU648330B2 (en) | 1994-04-21 |
| DK0540585T3 (da) | 1995-03-20 |
| AU8238691A (en) | 1992-02-18 |
| GB9016163D0 (en) | 1990-09-05 |
| CA2087982A1 (en) | 1992-01-25 |
| EP0540585A1 (de) | 1993-05-12 |
| WO1992001812A1 (en) | 1992-02-06 |
| EP0540585B1 (de) | 1994-12-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| REN | Ceased due to non-payment of the annual fee |