ATE102651T1 - METHOD OF GENE ENGINEERING PRODUCTION OF ANTIBODIES. - Google Patents

METHOD OF GENE ENGINEERING PRODUCTION OF ANTIBODIES.

Info

Publication number
ATE102651T1
ATE102651T1 AT88121675T AT88121675T ATE102651T1 AT E102651 T1 ATE102651 T1 AT E102651T1 AT 88121675 T AT88121675 T AT 88121675T AT 88121675 T AT88121675 T AT 88121675T AT E102651 T1 ATE102651 T1 AT E102651T1
Authority
AT
Austria
Prior art keywords
antibodies
gene engineering
hapten
antigen
engineering production
Prior art date
Application number
AT88121675T
Other languages
German (de)
Inventor
Andreas Dr Plueckthun
Arne Skerra
Original Assignee
Andreas Dr Plueckthun
Arne Skerra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=6343878&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE102651(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Andreas Dr Plueckthun, Arne Skerra filed Critical Andreas Dr Plueckthun
Application granted granted Critical
Publication of ATE102651T1 publication Critical patent/ATE102651T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Steroid Compounds (AREA)

Abstract

Functional antibody fragments can be produced in bacteria by coupling each of the genes for the individual chains to signal sequences which bring about the transport of the prepeptides through the cytoplasmic membrane, expressing the gene structures in a bacterium, and isolating the functional protein from the periplasmic space or the medium. The functional protein is advantageously isolated by affinity chromatography on an adsorbent which is loaded with the hapten or antigen, and elution with a solution of the hapten or antigen.
AT88121675T 1987-12-31 1988-12-24 METHOD OF GENE ENGINEERING PRODUCTION OF ANTIBODIES. ATE102651T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19873744595 DE3744595A1 (en) 1987-12-31 1987-12-31 METHOD FOR THE GENETIC ENGINEERING OF ANTIBODY
EP88121675A EP0324162B1 (en) 1987-12-31 1988-12-24 Method of producing antibodies by genetic engineering

Publications (1)

Publication Number Publication Date
ATE102651T1 true ATE102651T1 (en) 1994-03-15

Family

ID=6343878

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88121675T ATE102651T1 (en) 1987-12-31 1988-12-24 METHOD OF GENE ENGINEERING PRODUCTION OF ANTIBODIES.

Country Status (12)

Country Link
EP (1) EP0324162B1 (en)
JP (1) JP2771204B2 (en)
KR (1) KR970007861B1 (en)
AT (1) ATE102651T1 (en)
AU (1) AU628310B2 (en)
DE (2) DE3744595A1 (en)
DK (1) DK732988A (en)
ES (1) ES2063022T3 (en)
HU (1) HU212592B (en)
IE (1) IE62257B1 (en)
PT (1) PT89362B (en)
ZA (1) ZA889711B (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5618920A (en) * 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
GB9206318D0 (en) * 1992-03-24 1992-05-06 Cambridge Antibody Tech Binding substances
US6916605B1 (en) 1990-07-10 2005-07-12 Medical Research Council Methods for producing members of specific binding pairs
US7063943B1 (en) 1990-07-10 2006-06-20 Cambridge Antibody Technology Methods for producing members of specific binding pairs
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
SE9101433D0 (en) * 1991-05-13 1991-05-13 Marianne Hansson RECOMBINANT DNA SEQUENCE AND ITS USE
US5858657A (en) * 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
US6492160B1 (en) 1991-05-15 2002-12-10 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5962255A (en) * 1992-03-24 1999-10-05 Cambridge Antibody Technology Limited Methods for producing recombinant vectors
US6225447B1 (en) 1991-05-15 2001-05-01 Cambridge Antibody Technology Ltd. Methods for producing members of specific binding pairs
DE69230142T2 (en) 1991-05-15 2000-03-09 Cambridge Antibody Tech METHOD FOR PRODUCING SPECIFIC BINDING PAIRS
US7018809B1 (en) 1991-09-19 2006-03-28 Genentech, Inc. Expression of functional antibody fragments
US6146850A (en) * 1991-11-04 2000-11-14 Xoma Corporation Proteins encoding gelonin sequences
US5837491A (en) * 1991-11-04 1998-11-17 Xoma Corporation Polynucleotides encoding gelonin sequences
US5621083A (en) 1991-11-04 1997-04-15 Xoma Corporation Immunotoxins comprising ribosome-inactivating proteins
DK1024191T3 (en) * 1991-12-02 2008-12-08 Medical Res Council Preparation of autoantibodies displayed on phage surfaces from antibody segment libraries
EP0618977A4 (en) * 1991-12-18 1997-02-12 Univ Calgary Expression-secretion vectors for the production of biologically active fv fragments.
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US7368111B2 (en) 1995-10-06 2008-05-06 Cambridge Antibody Technology Limited Human antibodies specific for TGFβ2
GB9705376D0 (en) * 1997-03-14 1997-04-30 Harris William J Antibody fragment formulations and assay formats for detecting the presence an d concentration of analytes
US6492497B1 (en) 1999-04-30 2002-12-10 Cambridge Antibody Technology Limited Specific binding members for TGFbeta1
ATE382053T1 (en) * 2001-08-27 2008-01-15 Genentech Inc SYSTEM FOR ANTIBODIES EXPRESSION AND SYNTHESIS
EP1908769A1 (en) * 2001-08-27 2008-04-09 Genentech, Inc. A system for antibody expression and assembly
EP2102354B1 (en) * 2007-01-12 2017-08-16 Dow Global Technologies LLC Apparatus and methods for osmotically shocking cells
RU2677925C1 (en) 2013-12-12 2019-01-22 Конинклейке Филипс Н.В. Method to enable standard alternating current/direct current power adapters to operate in high magnetic fields

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8308235D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
EP0196864A3 (en) * 1985-03-25 1988-03-23 Cetus Corporation Alkaline phosphatase-mediated processing and secretion of recombinant proteins, dna sequences for use therein and cells transformed using such sequences
EP0234592A1 (en) * 1986-02-28 1987-09-02 Teijin Limited Plasmid containing DNA fragment coding for human immunoglobulin G Fc region protein and use thereof for production of said protein
JPS62201581A (en) * 1986-02-28 1987-09-05 Rikagaku Kenkyusho Production of novel plasmid, microbial cell and human immunoglobulin g fc fragment protein
WO1989000605A1 (en) * 1987-07-16 1989-01-26 Codon Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same
CA1341235C (en) * 1987-07-24 2001-05-22 Randy R. Robinson Modular assembly of antibody genes, antibodies prepared thereby and use

Also Published As

Publication number Publication date
AU2761788A (en) 1989-07-06
AU628310B2 (en) 1992-09-17
JPH02799A (en) 1990-01-05
JP2771204B2 (en) 1998-07-02
EP0324162A1 (en) 1989-07-19
IE883893L (en) 1989-06-30
DK732988D0 (en) 1988-12-30
PT89362B (en) 1993-08-31
DK732988A (en) 1989-07-01
DE3744595A1 (en) 1989-07-13
DE3888337D1 (en) 1994-04-14
HUT49169A (en) 1989-08-28
IE62257B1 (en) 1995-01-11
KR970007861B1 (en) 1997-05-17
EP0324162B1 (en) 1994-03-09
ES2063022T3 (en) 1995-01-01
HU212592B (en) 1996-08-29
KR890010203A (en) 1989-08-07
PT89362A (en) 1989-12-29
ZA889711B (en) 1989-09-27

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RZN Patent revoked