AT406324B - Plant-growth-promoting solution and process for its preparation - Google Patents
Plant-growth-promoting solution and process for its preparation Download PDFInfo
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- AT406324B AT406324B AT203098A AT203098A AT406324B AT 406324 B AT406324 B AT 406324B AT 203098 A AT203098 A AT 203098A AT 203098 A AT203098 A AT 203098A AT 406324 B AT406324 B AT 406324B
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Abstract
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AT 406 324 BAT 406 324 B
Die Erfindung betrifft eine das Wachstum von Pflanzen fördernde Lösung und ein Verfahren zum Herstellen dieser das Wachstum von Pflanzen fördernden Lösung.The invention relates to a plant-promoting solution and a method for producing this plant-promoting solution.
Pfianzenwachstum kann auf verschiedene Art und Weise gefördert werden. So besteht die Möglichkeit, den Pflanzen hinreichend Nährstoffe (enthaltend die erforderlichen Makro- und Mikroelemente) zuzuführen. Des weiteren ist es möglich, die optimale Temperatur für das Wachstum und die optimale Belichtung sicherzustellen.Plant growth can be promoted in a number of ways. So there is the possibility to supply the plants with sufficient nutrients (containing the necessary macro and micro elements). It is also possible to ensure the optimal temperature for growth and optimal exposure.
Es gibt weitere Substanzen, deren Anwesenheit das Wachstum von Pflanzen unterstützt. Zu diesen zählen verschiedene bekannte Wachstumshormone, die im allgemeinen von den Pflanzen selbst erzeugt werden. Darüber hinaus gibt es andere, teilweise unbekannte Substanzen, die eine das Wachstum von Pflanzen unterstützende Wirkung haben und die von anderen Organismen erzeugt werden, wie beispielsweise von Mikroorganismen.There are other substances whose presence supports the growth of plants. These include various known growth hormones, which are generally produced by the plants themselves. In addition, there are other substances, some of which are unknown, that support the growth of plants and are produced by other organisms, such as microorganisms.
Es ist bekannt, daß viele Mikroorganismen im Boden zusammen mit Pflanzen leben, teils in Symbiose und teils nur in losem Kontakt mit den Pflanzen. Einige dieser Mikroorganismen unterstützen das Pflanzenwachstum, indem sie Luftstickstoff in Form von Nitraten binden und die Pflanzen die sc gebildeten Nitrate als wachstumsfördemde Stoffe in sich aufnehmen. Ein Mitglied dieser Gruppe von Bakterien ist das Bakterium Rhizobium.It is known that many microorganisms live together with plants in the soil, partly in symbiosis and partly only in loose contact with the plants. Some of these microorganisms support plant growth by binding atmospheric nitrogen in the form of nitrates and the plants absorbing the nitrates formed as growth-promoting substances. A member of this group of bacteria is the bacterium rhizobium.
Der Erfindung liegt die Aufgabe zugrunde, eine Lösung zur Verfügung zu stellen, die beim Bewässern von Pflanzen mit der Lösung eine wachstumsfördemde Wirkung hat. Des weiteren liegt der Erfindung die Aufgabe zugrunde, ein Verfahren zum Herstellen einer solchen Lösung zur Verfügung zu Stellen-Gelöst wird diese Aufgabe zunächst mit den Merkmalen der Ansprüche 1 und 3.The invention has for its object to provide a solution that has a growth-promoting effect when watering plants with the solution. Furthermore, the invention is based on the object of providing a method for producing such a solution. This object is initially achieved with the features of claims 1 and 3.
Bevorzugte und vorteilhafte Ausgestaltungen der Erfindung sowohl was die Lösung, als auch was deren Herstellung anlangt, sind Gegenstand der Unteransprüche -Preferred and advantageous refinements of the invention, both in terms of the solution and in terms of its manufacture, are the subject of the dependent claims.
Die Erfindung beruht auf der Erkenntnis, daß Bakterien der Gattung Rhizobium Substanzen als Stoffwechselprodukte produzieren, die das Wachstum von verschiedenen Pflanzen (einschließlich Hülsenfruchtpflanzen) fördern.The invention is based on the knowledge that bacteria of the genus Rhizobium produce substances as metabolites which promote the growth of various plants (including legume plants).
Besonders bevorzugt im Rahmen der Erfindung ist das Bakterium Rhizobium meliloti.The bacterium Rhizobium meliloti is particularly preferred in the context of the invention.
Werden Pflanzen mit der erfindungsgemäßen bzw. der nach dem erfindungsgemäßen Verfahren hergestellten Lösung gegossen, dann wachsen die so gegossenen Pflanzen nicht nur höher, sondern sind auch insgesamt stärker.If plants are watered with the solution according to the invention or the solution produced by the process according to the invention, the plants thus watered not only grow taller but are also stronger overall.
Nachstehend wird beispielhaft ein Verfahren zum Herstellen der erfindungsgemäßen Lösung unter Verwendung des Bakterium Rhizobium meliloti und die Ergebnisse von Versuchen mit der erfindungsgemäßen Lösung beschrieben. 1. Herstellen der Lösung: 1a. Züchten von Rhizobium meliloti:A method for producing the solution according to the invention using the bacterium Rhizobium meliloti and the results of experiments with the solution according to the invention are described below by way of example. 1. Preparation of the solution: 1a. Breeding Rhizobium meliloti:
Das Bakterium wurde in einem Medium 48 Stunden lang unter Schütteln bei 30 °C in einem wässerigen Medium kultiviert, das folgende Zusammensetzung hatte: 0,1% Hefeextrakt, 0,5% NaCI, 1 mM MgS04-6H20, 1 mM CaCI2. H20.The bacterium was cultured in a medium with shaking at 30 ° C for 48 hours in an aqueous medium having the following composition: 0.1% yeast extract, 0.5% NaCl, 1mM MgSO4-6H20, 1mM CaCl2. H20.
Das Kulturmedium hatte einen pH-Wert von 7,0 bis 7,2.The culture medium had a pH of 7.0 to 7.2.
Nach 48 Stunden Kultivieren hatte das Kulturmedium eine optische Dichte von 0,625 bei 600 nm und die Bakterien wurden durch Zentrifugieren bei 2500 U'1 10 Minuten lang bei 4°C gesammelt. Die gesammelten Bakterien wurden mit physiologischer Kochsalzlösung gewaschen und dann erneut zentrifugiert. Die so gewonnenen Bakterien wurden in physiologischer Kochsalzlösung suspendiert. 2 1 b. 11nmobilisieren von Rhizobium melilotiAfter 48 hours of culture, the culture medium had an optical density of 0.625 at 600 nm and the bacteria were collected by centrifugation at 2500 rpm for 10 minutes at 4 ° C. The collected bacteria were washed with physiological saline and then centrifuged again. The bacteria thus obtained were suspended in physiological saline. 2 1 b. Mobilize Rhizobium meliloti
Zum Immobilisierer der Bakterien wurde eine Lösung enthaltend 0,1% Natriumalginat (Handelsbezeichnung Protanal F250, Hersteller Protan, Drammen, Norwegen), 3,0% Carrageen (Handelsbezeichnung Carragenat No. C-1263, Hersteller Sigma Co.) und 0,01% EDTA (Ethylendiamintetraessigsäure) bei 37°C vorinkubiert. In der so hergestellten Immobilisierungslösung wurde eine 5%-ige Bakteriensuspension erzeugt- Diese Suspension wurde tropfenweise bei einer Temperatur von 37°C zu dem 4- fachen Volumen einer 100mM KC1-Lösung zugetropft. Nachdem 1,5 Stunden lang bei einer Temperatur von 24eC vorsichtig gerührt wurde, wurden die verfestigten Kügelchen, auf welchen die Bakterien immobilisiert waren, absetzen gelassen. Die so gewonnenen Kügelchen mit den in ihnen immobilisierten Bakterien wurden mit dem 5- fachenA solution containing 0.1% sodium alginate (trade name Protanal F250, manufacturer Protan, Drammen, Norway), 3.0% carrageenan (trade name Carragenat No. C-1263, manufacturer Sigma Co.) and 0.01% was used to immobilize the bacteria. Pre-incubated EDTA (ethylenediaminetetraacetic acid) at 37 ° C. A 5% bacterial suspension was generated in the immobilization solution thus prepared. This suspension was added dropwise at a temperature of 37 ° C. to 4 times the volume of a 100 mM KC1 solution. After stirring gently at a temperature of 24eC for 1.5 hours, the solidified beads on which the bacteria were immobilized were allowed to settle. The beads thus obtained, with the bacteria immobilized in them, were increased 5-fold
Claims (10)
Priority Applications (1)
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AT203098A AT406324B (en) | 1998-12-01 | 1998-12-01 | Plant-growth-promoting solution and process for its preparation |
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AT203098A AT406324B (en) | 1998-12-01 | 1998-12-01 | Plant-growth-promoting solution and process for its preparation |
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AT406324B true AT406324B (en) | 2000-04-25 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001026465A1 (en) * | 1999-10-08 | 2001-04-19 | Mcgill University | Use of lipo-chitooligosaccharides for increasing photosynthesis in plants and corresponding methods and compositions |
US7250068B1 (en) | 2000-10-06 | 2007-07-31 | Mcgill University | Method of increasing photosynthesis in plants comprising an exposure thereof to lipochitooligosaccharides and compositions therefor |
CN108441438A (en) * | 2018-02-06 | 2018-08-24 | 杭州师范大学 | Rhizobium WYJ-E13 and its application in preparing RADIX CURCUMAE growth promoter |
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EP0064720A2 (en) * | 1981-05-04 | 1982-11-17 | The Research And Development Institute Inc. At Montana State University | Compositions containing and methods of use of an infectivity-cured Hr plasmid-bearing microorganism |
EP0242474A2 (en) * | 1986-04-21 | 1987-10-28 | W.R. Grace & Co.-Conn. | Rhizobium inoculants |
WO1990015138A1 (en) * | 1989-05-30 | 1990-12-13 | Wisconsin Alumni Research Foundation | Recombinant rhizobium bacteria inoculants |
WO1994025568A1 (en) * | 1993-04-23 | 1994-11-10 | Her Majesty The Queen In Right Of Canada As Represented By The Department Of Agriculture And Agri-Food Canada | Cold-tolerant strains of rhizobium species |
WO1995017806A1 (en) * | 1993-12-29 | 1995-07-06 | Philom Bios Inc. | Methods and compositions for increasing the benefits of rhizobium inoculation to legume crop productivity |
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1998
- 1998-12-01 AT AT203098A patent/AT406324B/en not_active IP Right Cessation
Patent Citations (5)
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EP0064720A2 (en) * | 1981-05-04 | 1982-11-17 | The Research And Development Institute Inc. At Montana State University | Compositions containing and methods of use of an infectivity-cured Hr plasmid-bearing microorganism |
EP0242474A2 (en) * | 1986-04-21 | 1987-10-28 | W.R. Grace & Co.-Conn. | Rhizobium inoculants |
WO1990015138A1 (en) * | 1989-05-30 | 1990-12-13 | Wisconsin Alumni Research Foundation | Recombinant rhizobium bacteria inoculants |
WO1994025568A1 (en) * | 1993-04-23 | 1994-11-10 | Her Majesty The Queen In Right Of Canada As Represented By The Department Of Agriculture And Agri-Food Canada | Cold-tolerant strains of rhizobium species |
WO1995017806A1 (en) * | 1993-12-29 | 1995-07-06 | Philom Bios Inc. | Methods and compositions for increasing the benefits of rhizobium inoculation to legume crop productivity |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001026465A1 (en) * | 1999-10-08 | 2001-04-19 | Mcgill University | Use of lipo-chitooligosaccharides for increasing photosynthesis in plants and corresponding methods and compositions |
EP1554932A2 (en) * | 1999-10-08 | 2005-07-20 | McGILL UNIVERSITY | Use of lipo-chitooligosaccharides for increasing photosynthesis in plants and corresponding methods and compositions |
EP1554932A3 (en) * | 1999-10-08 | 2006-03-29 | McGILL UNIVERSITY | Use of lipo-chitooligosaccharides for increasing photosynthesis in plants and corresponding methods and compositions |
US7637980B2 (en) | 1999-10-08 | 2009-12-29 | Mcgill University | Method of increasing photosynthesis in plants comprising an exposure thereof to lipochitooligosaccharides and compositions therefor |
EP2907389A1 (en) * | 1999-10-08 | 2015-08-19 | McGill University | Use of lipo-chitooligosaccharides for increasing photosynthesis in plants and corresponding methods and compositions |
US7250068B1 (en) | 2000-10-06 | 2007-07-31 | Mcgill University | Method of increasing photosynthesis in plants comprising an exposure thereof to lipochitooligosaccharides and compositions therefor |
CN108441438A (en) * | 2018-02-06 | 2018-08-24 | 杭州师范大学 | Rhizobium WYJ-E13 and its application in preparing RADIX CURCUMAE growth promoter |
CN108441438B (en) * | 2018-02-06 | 2020-10-09 | 杭州师范大学 | Rhizobium WYJ-E13 and application thereof in preparation of curcuma wenyujin growth promoter |
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ATA203098A (en) | 1999-09-15 |
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