AR127146A1 - COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS - Google Patents

COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS

Info

Publication number
AR127146A1
AR127146A1 ARP220102579A ARP220102579A AR127146A1 AR 127146 A1 AR127146 A1 AR 127146A1 AR P220102579 A ARP220102579 A AR P220102579A AR P220102579 A ARP220102579 A AR P220102579A AR 127146 A1 AR127146 A1 AR 127146A1
Authority
AR
Argentina
Prior art keywords
plant
contact
genetically modified
explant
methods
Prior art date
Application number
ARP220102579A
Other languages
Spanish (es)
Inventor
Yurong Chen
Byung Kang
- Kumpf Jennifer Guk
Mary Ann Saltarikos
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR127146A1 publication Critical patent/AR127146A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation

Abstract

La invención proporciona composiciones y métodos novedosos para regenerar una planta monocotiledónea o parte de la planta genéticamente modificada en contacto con un medio de regeneración que tiene una baja concentración de sal. Los métodos proporcionados en el presente documento pueden incluir la transformación de al menos una célula vegetal del explante de embrión con un polinucleótido heterólogo, cultivar el explante de embrión en contacto con un primer medio de inducción de brotes que una auxina y una citoquinina y regenerar una planta o parte de la planta genéticamente modificada a partir de una célula vegetal o explante transformado o editado. Reivindicación 1: Un método para producir una planta monocotiledónea o parte de la planta genéticamente modificada que comprende: introducir una molécula polinucleotídica heteróloga en al menos una célula de un explante de embrión de semilla monocotiledónea que comprende tejido meristemático cultivar el explante de embrión en contacto con un primer medio de inducción de brotes que comprende una primera auxina y una primera citoquinina, y regenerar la planta monocotiledónea o parte de la planta genéticamente modificada a partir del explante de embrión cultivado en contacto con un medio de regeneración, en donde el medio de regeneración tiene una baja concentración de sal.The invention provides novel compositions and methods for regenerating a genetically modified monocotyledonous plant or plant part in contact with a regeneration medium having a low salt concentration. The methods provided herein may include transforming at least one plant cell of the embryo explant with a heterologous polynucleotide, culturing the embryo explant in contact with a first sprout induction medium containing an auxin and a cytokinin, and regenerating a genetically modified plant or part of a plant from a transformed or edited plant cell or explant. Claim 1: A method of producing a genetically modified monocot plant or plant part comprising: introducing a heterologous polynucleotide molecule into at least one cell of a monocot seed embryo explant comprising meristematic tissue cultivating the embryo explant in contact with a first shoot induction medium comprising a first auxin and a first cytokinin, and regenerating the monocotyledonous plant or part of the genetically modified plant from the embryo explant cultured in contact with a regeneration medium, wherein the regeneration medium It has a low concentration of salt.

ARP220102579A 2021-09-27 2022-09-26 COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS AR127146A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163248960P 2021-09-27 2021-09-27

Publications (1)

Publication Number Publication Date
AR127146A1 true AR127146A1 (en) 2023-12-20

Family

ID=85721327

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220102579A AR127146A1 (en) 2021-09-27 2022-09-26 COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS

Country Status (2)

Country Link
AR (1) AR127146A1 (en)
WO (1) WO2023049903A1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003041491A2 (en) * 2001-11-13 2003-05-22 Agricore United Doubled haploid production and genetic transformation
US7354760B2 (en) * 2001-12-26 2008-04-08 University Of Central Florida Research Foundation, Inc. Expression of protective antigens in transgenic chloroplasts
US8581035B2 (en) * 2006-08-31 2013-11-12 Monsanto Technology Llc Plant transformation without selection
US11807846B2 (en) * 2012-04-29 2023-11-07 Monsanto Technology Llc Methods for transforming corn explants
US20210054390A1 (en) * 2018-05-24 2021-02-25 Monsanto Technology Llc Genome editing in plants

Also Published As

Publication number Publication date
WO2023049903A1 (en) 2023-03-30
WO2023049903A8 (en) 2023-12-14

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