AR126939A1 - HIGH MOLECULAR WEIGHT MODIFIED dsRNA COMPOSITIONS RELATED TO BIOCIDES - Google Patents

HIGH MOLECULAR WEIGHT MODIFIED dsRNA COMPOSITIONS RELATED TO BIOCIDES

Info

Publication number
AR126939A1
AR126939A1 ARP220102359A ARP220102359A AR126939A1 AR 126939 A1 AR126939 A1 AR 126939A1 AR P220102359 A ARP220102359 A AR P220102359A AR P220102359 A ARP220102359 A AR P220102359A AR 126939 A1 AR126939 A1 AR 126939A1
Authority
AR
Argentina
Prior art keywords
dsrnas
molecular weight
high molecular
fertility
reduces
Prior art date
Application number
ARP220102359A
Other languages
Spanish (es)
Inventor
Darren H Wakefield
Grace B Arhancet
Vladimir Trubetskoy
Juan P Arhancet
Jason Klein
Original Assignee
nanoSUR LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by nanoSUR LLC filed Critical nanoSUR LLC
Publication of AR126939A1 publication Critical patent/AR126939A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P23/00—Chemosterilants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/02—Acaricides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Abstract

Se describen los ARN de doble hebra modificados químicamente después de la transcripción (ARNbcM) que tienen una modificación polialquiloxilo de peso molecular elevado en la posición 2’-OH. Esta modificación proporciona una mayor biodisponibilidad del compuesto, mejor estabilidad del compuesto y proporciona una mayor estabilidad frente al nucleasas. Los ARNbcM se pueden producir de manera rentable de una manera fácilmente escalable. Se describen composiciones de ARNbcM que tienen nucleótidos modificados químicamente, de modo que los ARNbcM se modifican para contener polímeros de polialquiloxilo de peso molecular elevado. Se describen métodos sintéticos para hacer eficientemente estos ARNbcM y métodos de uso de ARNbcM. Reivindicación 43: Un método de modificación de la expresión de un polinucleótido de interés en cualquiera de los siguientes: un insecto, un ácaro, un hongo o una mala hierba que comprende administrar una composición de una cualquiera de las reivindicaciones 1 - 9. Reivindicación 48: El método de las reivindicaciones 43 - 47, donde la expresión modificada aumenta la mortalidad o induce retraso en el crecimiento, o detiene el desarrollo de estadios o reduce la fertilidad del insecto diana. Reivindicación 49: El método de una cualquiera de las reivindicaciones 43 - 48, donde la expresión modificada reduce la fertilidad de los insectos lepidópteros. Reivindicación 51: El método de una cualquiera de las reivindicaciones 43 - 48, donde la expresión aumenta la mortalidad o induce retraso en el crecimiento, o detiene el desarrollo de estadios o reduce la fertilidad de los insectos coleópteros. Reivindicación 53: El método de la reivindicación 43, donde la mala hierba es Palmer Amaranth. Reivindicación 54: El método de la reivindicación 43, donde el hongo es Fusarium Graminearum o Botrytis. Reivindicación 55: El método de la reivindicación 43, donde el ácaro es ácaro Varroa.Described are post-transcriptionally chemically modified double-stranded RNAs (dsRNAs) that have a high molecular weight polyalkyloxyl modification at the 2-OH position. This modification provides greater bioavailability of the compound, better stability of the compound and provides greater stability against nucleases. dsRNAs can be produced cost-effectively in an easily scalable manner. Disclosed are MdsRNA compositions that have chemically modified nucleotides, such that the MdsRNAs are modified to contain high molecular weight polyalkyloxy polymers. Synthetic methods to efficiently make these dsRNAs and methods of using dsRNAs are described. Claim 43: A method of modifying the expression of a polynucleotide of interest in any of the following: an insect, a mite, a fungus or a weed comprising administering a composition of any one of claims 1 - 9. Claim 48 : The method of claims 43 - 47, wherein the modified expression increases mortality or induces growth retardation, or stops stage development or reduces fertility of the target insect. Claim 49: The method of any one of claims 43-48, wherein the modified expression reduces the fertility of lepidopteran insects. Claim 51: The method of any one of claims 43 - 48, wherein the expression increases mortality or induces growth retardation, or arrests the development of stages or reduces the fertility of coleopteran insects. Claim 53: The method of claim 43, wherein the weed is Palmer Amaranth. Claim 54: The method of claim 43, wherein the fungus is Fusarium Graminearum or Botrytis. Claim 55: The method of claim 43, wherein the mite is Varroa mite.

ARP220102359A 2021-08-31 2022-08-31 HIGH MOLECULAR WEIGHT MODIFIED dsRNA COMPOSITIONS RELATED TO BIOCIDES AR126939A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163239165P 2021-08-31 2021-08-31

Publications (1)

Publication Number Publication Date
AR126939A1 true AR126939A1 (en) 2023-11-29

Family

ID=83508899

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220102359A AR126939A1 (en) 2021-08-31 2022-08-31 HIGH MOLECULAR WEIGHT MODIFIED dsRNA COMPOSITIONS RELATED TO BIOCIDES

Country Status (3)

Country Link
AR (1) AR126939A1 (en)
CA (1) CA3228823A1 (en)
WO (1) WO2023034849A2 (en)

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066605A2 (en) * 1999-04-30 2000-11-09 Cyclops Genome Sciences Limited Polynucleotides
US20040167090A1 (en) * 2003-02-21 2004-08-26 Monahan Sean D. Covalent modification of RNA for in vitro and in vivo delivery
CA2693280C (en) 2004-04-09 2017-09-12 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
TW200836762A (en) * 2006-11-27 2008-09-16 Enzon Pharmaceuticals Inc Polymeric short interfering RNA conjugates
KR101982126B1 (en) 2010-03-08 2019-05-27 몬산토 테크놀로지 엘엘씨 Polynucleotide Molecules for Gene Regulation in Plants
US9428791B2 (en) * 2012-08-14 2016-08-30 The Board Of Trustees Of The Leland Stanford Junior University Probes of RNA structure and methods for using the same
HUE053033T2 (en) 2013-06-19 2021-06-28 Apse Llc Compositions and methods using capsids resistant to hydrolases
US11441147B2 (en) 2013-07-10 2022-09-13 Basf Se RNAi for the control of phytopathogenic fungi and oomycetes by inhibiting the expression of CYP51 genes
WO2016043960A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
BR112019008856A2 (en) 2016-11-07 2019-07-09 nanoSUR LLC Post-transcriptionally modified chemically modified double stranded ribbons
PE20200691A1 (en) 2017-05-01 2020-06-11 Donald Danforth Plant Science Center ARNI APPROACH TO PROTECTING CROPS AGAINST PESTS
CN111033816B (en) 2017-08-28 2022-09-13 株式会社村田制作所 Nonaqueous electrolyte battery and communication device
US11572557B2 (en) * 2018-01-29 2023-02-07 The Board Of Trustees Of The Leland Stanford Junior University Photoreversible acylation reagents
BR112021002792A2 (en) 2018-08-13 2021-06-29 Pioneer Hi-Bred International, Inc. new insecticidal toxin receptors and methods of use
US20220307024A1 (en) * 2019-06-17 2022-09-29 Alnylam Pharmaceuticals, Inc. Delivery of oligonucleotides to the striatum
DE102019212345B4 (en) 2019-08-19 2024-02-08 Robert Bosch Gmbh Electromechanically driven brake pressure generator

Also Published As

Publication number Publication date
CA3228823A1 (en) 2023-03-09
WO2023034849A3 (en) 2023-05-19
WO2023034849A8 (en) 2024-03-07
WO2023034849A2 (en) 2023-03-09

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