AR124613A1 - NEW PSYCHOSE-6-PHOSPHATE PHOSPHASE, COMPOSITION FOR PRODUCING PSYCHOSE INCLUDING PHOSPHASE AND METHOD FOR PRODUCING PSYCHOSE USING PHOSPHASE - Google Patents

NEW PSYCHOSE-6-PHOSPHATE PHOSPHASE, COMPOSITION FOR PRODUCING PSYCHOSE INCLUDING PHOSPHASE AND METHOD FOR PRODUCING PSYCHOSE USING PHOSPHASE

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Publication number
AR124613A1
AR124613A1 ARP180103591A ARP180103591A AR124613A1 AR 124613 A1 AR124613 A1 AR 124613A1 AR P180103591 A ARP180103591 A AR P180103591A AR P180103591 A ARP180103591 A AR P180103591A AR 124613 A1 AR124613 A1 AR 124613A1
Authority
AR
Argentina
Prior art keywords
psychose
phosphate
producing
phosphorylase
phosphatase
Prior art date
Application number
ARP180103591A
Other languages
Spanish (es)
Inventor
Su Jin Kim
Yang Hee Kim
So Kim
- Kim Jungeun Hyeong
Eul Park
- Kim Seong Bo Soo
Eun Jung Choi
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of AR124613A1 publication Critical patent/AR124613A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)

Abstract

La presente descripción se refiere a una nueva psicosa-6-fosfato fosfatasa, una composición para producir psicosa que comprende la fosfatasa, y un método para producir psicosa usando la fosfatasa. Reivindicación 1: Una psicosa-6-fosfato fosfatasa que se deriva del género Meiothermus y que tiene capacidad de desfoforilación selectiva de psicosa-6-fosfato. Reivindicación 3: La psicosa-6-fosfato fosfatasa de acuerdo con la Reivindicación 1, donde la psicosa-6-fosfato fosfatasa comprende una secuencia de aminoácidos de la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, o SEQ ID Nº 4. Reivindicación 8: Una composición para producir psicosa, que comprende la psicosa-6-fosfato fosfatasa de acuerdo con cualquiera de las reivindicaciones 1 a 5, un microorganismo que expresa el mismo, o un cultivo de microorganismo. Reivindicación 9: La composición para producir psicosa de acuerdo con la Reivindicación 8, donde la composición además comprende: (a) (i) almidón, maltodextrina, sacarosa, o una combinación de estos; (ii) fosfato; (iii) fructosa-6-fosfato-3-epimerasa; (iv) glucosa-6-fosfato isomerasa; (v) fosfoglucomutasa o glucoquinasa; (vi) al menos una seleccionada del grupo que consiste en a-glucan fosforilasa, almidón fosforilasa, maltodextrina fosforilasa, sacarosa fosforilasa, a-amilasa, pululanasa, isoamilasa, glucoamilasa, o sucrasa; o (b) un microorganismo que expresa las enzimas de (a) o un cultivo del microrganismo. Reivindicación 18: Un método para producir psicosa, que comprende la reacción de almidón, maltodextrina, sacarosa, o una combinación de estos, y fosfato con (a) inositol-mono-fosfatasa; fructosa-6-fosfato-3-epimerasa; glucosa-6-fosfato isomerasa; fosfoglucomutasa o glucoquinasa; y a-glucan fosforilasa, almidón fosforilasa, maltodextrina fosforilasa, sacarosa fosforilasa, a-amilasa, pululanasa, isoamilasa, glucoamilasa, o sucrasa; o (b) un microorganismo que expresa las enzimas de (a) o un cultivo del microorganismo.The present disclosure relates to a new psychose-6-phosphate phosphatase, a composition for producing psychose comprising the phosphatase, and a method for producing psychose using the phosphatase. Claim 1: A psychose-6-phosphate phosphatase which is derived from the genus Meiothermus and which has the ability to selectively dephosphorylate psychose-6-phosphate. Claim 3: The psychose-6-phosphate phosphatase according to Claim 1, wherein the psychose-6-phosphate phosphatase comprises an amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID No. 4. Claim 8: A composition for producing psychose, comprising the psychose-6-phosphate phosphatase according to any of claims 1 to 5, a microorganism expressing the same, or a microorganism culture. Claim 9: The composition for producing psychose according to Claim 8, wherein the composition further comprises: (a) (i) starch, maltodextrin, sucrose, or a combination thereof; (ii) phosphate; (iii) fructose-6-phosphate-3-epimerase; (iv) glucose-6-phosphate isomerase; (v) phosphoglucomutase or glucokinase; (vi) at least one selected from the group consisting of α-glucan phosphorylase, starch phosphorylase, maltodextrin phosphorylase, sucrose phosphorylase, α-amylase, pullulanase, isoamylase, glucoamylase, or sucrase; or (b) a microorganism that expresses the enzymes of (a) or a culture of the microorganism. Claim 18: A method for producing psychose, comprising the reaction of starch, maltodextrin, sucrose, or a combination of these, and phosphate with (a) inositol-mono-phosphatase; fructose-6-phosphate-3-epimerase; glucose-6-phosphate isomerase; phosphoglucomutase or glucokinase; and a-glucan phosphorylase, starch phosphorylase, maltodextrin phosphorylase, sucrose phosphorylase, a-amylase, pullulanase, isoamylase, glucoamylase, or sucrase; or (b) a microorganism that expresses the enzymes of (a) or a culture of the microorganism.

ARP180103591A 2017-12-08 2018-12-07 NEW PSYCHOSE-6-PHOSPHATE PHOSPHASE, COMPOSITION FOR PRODUCING PSYCHOSE INCLUDING PHOSPHASE AND METHOD FOR PRODUCING PSYCHOSE USING PHOSPHASE AR124613A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20170167873 2017-12-08

Publications (1)

Publication Number Publication Date
AR124613A1 true AR124613A1 (en) 2023-04-19

Family

ID=66751529

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180103591A AR124613A1 (en) 2017-12-08 2018-12-07 NEW PSYCHOSE-6-PHOSPHATE PHOSPHASE, COMPOSITION FOR PRODUCING PSYCHOSE INCLUDING PHOSPHASE AND METHOD FOR PRODUCING PSYCHOSE USING PHOSPHASE

Country Status (3)

Country Link
KR (1) KR102114865B1 (en)
AR (1) AR124613A1 (en)
WO (1) WO2019112368A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102509561B1 (en) * 2020-12-21 2023-03-14 주식회사 삼양사 Phosphatase with high substrate specificity and use of the same
CN114601745B (en) * 2022-03-25 2023-06-27 上海龙殷生物科技有限公司 Skin care product raw material, cosmetic, preparation method and application

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014060425A1 (en) 2012-10-17 2014-04-24 Tetra Laval Holdings & Finance S.A. A tube holding element
WO2014060452A1 (en) * 2012-10-18 2014-04-24 Universiteit Gent A thermostable sucrose and sucrose-6'-phosphate phosphorylase
KR101455759B1 (en) * 2013-04-23 2014-10-28 씨제이제일제당(주) D-psicose 3-epimerase variants and production method of D-psicose using them
KR20140140215A (en) * 2013-05-28 2014-12-09 경상대학교산학협력단 Corynebacterium including polynucleotide coding psicose 3-epimerase and producing method for Psicose using the same
KR101577147B1 (en) * 2014-10-01 2015-12-11 경상대학교산학협력단 Preparing method for psicose
KR101695830B1 (en) * 2014-10-30 2017-01-12 주식회사 삼양사 Expression system for psicose epimerase and production for psicose using the same

Also Published As

Publication number Publication date
WO2019112368A1 (en) 2019-06-13
KR20190068470A (en) 2019-06-18
KR102114865B1 (en) 2020-05-27

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