AR123184A1 - COMPOSITIONS AND METHODS TO IMPROVE RESISTANCE TO NORTHERN LEAF BLIGHT IN CORN - Google Patents

COMPOSITIONS AND METHODS TO IMPROVE RESISTANCE TO NORTHERN LEAF BLIGHT IN CORN

Info

Publication number
AR123184A1
AR123184A1 ARP210102215A ARP210102215A AR123184A1 AR 123184 A1 AR123184 A1 AR 123184A1 AR P210102215 A ARP210102215 A AR P210102215A AR P210102215 A ARP210102215 A AR P210102215A AR 123184 A1 AR123184 A1 AR 123184A1
Authority
AR
Argentina
Prior art keywords
leaf blight
northern leaf
resistance
recombinant dna
dna construct
Prior art date
Application number
ARP210102215A
Other languages
Spanish (es)
Inventor
Shane E Abbitt
Bailin Li
Shawn Thatcher
Yurong Li
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR123184A1 publication Critical patent/AR123184A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Abstract

La divulgación se refiere a métodos y composiciones para identificar y seleccionar plantas de maíz con resistencia mejorada al tizón foliar norteño. También son una característica las plantas de maíz generadas mediante los métodos descritos en el presente documento. Reivindicación 1: Una construcción de ADN recombinante que comprende un polinucleótido aislado unido operativamente a al menos una secuencia reguladora, en donde dicho polinucleótido aislado comprende al menos una secuencia de nucleótidos que codifica un polipéptido capaz de conferir o mejorar la resistencia al tizón foliar norteño, en donde el polipéptido tiene una secuencia de aminoácidos con al menos un 90% de identidad de secuencia respecto de la SEQ ID Nº 100, y en donde el polipéptido capaz de conferir o mejorar la resistencia al tizón foliar norteño tiene un sitio de acción o un modo de acción diferente en comparación con la SEQ ID Nº 97. Reivindicación 9: Un proceso de alteración del nivel de expresión de una proteína capaz de conferir resistencia al tizón foliar norteño en una célula de maíz que comprende: a. transformar una célula de maíz con la construcción de ADN recombinante de la reivindicación 1; y b. cultivar la célula de maíz transformada en condiciones adecuadas para la expresión de la construcción de ADN recombinante, en donde la expresión de la construcción de ADN recombinante da lugar a la producción de niveles alterados de una proteína capaz de conferir resistencia al tizón foliar norteño en la célula de maíz transformada cuando se compara con los niveles de expresión en una planta de maíz de tipo silvestre con resistencia al tizón foliar norteño.The disclosure relates to methods and compositions for identifying and selecting maize plants with improved resistance to northern leaf blight. Also a feature are corn plants generated by the methods described herein. Claim 1: A recombinant DNA construct comprising an isolated polynucleotide operatively linked to at least one regulatory sequence, wherein said isolated polynucleotide comprises at least one nucleotide sequence encoding a polypeptide capable of conferring or enhancing resistance to northern leaf blight, wherein the polypeptide has an amino acid sequence with at least 90% sequence identity to SEQ ID No. 100, and wherein the polypeptide capable of conferring or enhancing resistance to northern leaf blight has a site of action or a different mode of action compared to SEQ ID No. 97. Claim 9: A process for altering the expression level of a protein capable of conferring resistance to northern leaf blight in a maize cell comprising: a. transforming a maize cell with the recombinant DNA construct of claim 1; and b. culturing the transformed maize cell under conditions suitable for expression of the recombinant DNA construct, wherein expression of the recombinant DNA construct results in the production of altered levels of a protein capable of conferring resistance to northern leaf blight on the transformed maize cell when compared to expression levels in a wild-type maize plant with resistance to northern leaf blight.

ARP210102215A 2020-08-10 2021-08-09 COMPOSITIONS AND METHODS TO IMPROVE RESISTANCE TO NORTHERN LEAF BLIGHT IN CORN AR123184A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202063063578P 2020-08-10 2020-08-10

Publications (1)

Publication Number Publication Date
AR123184A1 true AR123184A1 (en) 2022-11-09

Family

ID=77520807

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP210102215A AR123184A1 (en) 2020-08-10 2021-08-09 COMPOSITIONS AND METHODS TO IMPROVE RESISTANCE TO NORTHERN LEAF BLIGHT IN CORN

Country Status (7)

Country Link
EP (1) EP4192232A2 (en)
CN (1) CN116234816A (en)
AR (1) AR123184A1 (en)
BR (1) BR112023002602A2 (en)
CA (1) CA3189779A1 (en)
MX (1) MX2023001641A (en)
WO (1) WO2022035653A2 (en)

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Also Published As

Publication number Publication date
CA3189779A1 (en) 2022-02-17
CN116234816A (en) 2023-06-06
BR112023002602A2 (en) 2023-04-04
WO2022035653A3 (en) 2022-03-24
EP4192232A2 (en) 2023-06-14
MX2023001641A (en) 2023-03-08
WO2022035653A2 (en) 2022-02-17

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