AR113432A1 - TARGETED INTEGRATION OF NUCLEIC ACIDS - Google Patents
TARGETED INTEGRATION OF NUCLEIC ACIDSInfo
- Publication number
- AR113432A1 AR113432A1 ARP180103814A ARP180103814A AR113432A1 AR 113432 A1 AR113432 A1 AR 113432A1 AR P180103814 A ARP180103814 A AR P180103814A AR P180103814 A ARP180103814 A AR P180103814A AR 113432 A1 AR113432 A1 AR 113432A1
- Authority
- AR
- Argentina
- Prior art keywords
- targeted integration
- host cell
- nucleic acids
- rrs
- nucleotide sequence
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente se refiere a las células huésped de integración dirigida (TI) adecuadas para la expresión de proteínas recombinantes, así como también a los métodos para producir y usar dichas células huésped de TI. Reivindicación 1: Una célula huésped de integración dirigida (TI) que comprende una secuencia de nucleótidos exógena integrada a un sitio de integración dentro de un locus específico del genoma de la célula huésped, en donde el locus es al menos aproximadamente 90% homólogo a una secuencia seleccionada de las secuencias con números de identificación: 1 - 7. Reivindicación 13: La célula huésped de TI de cualquiera de las reivindicaciones 1 - 12, en donde la secuencia de nucleótidos exógena comprende una o más secuencias de reconocimiento de recombinación (RRS), en donde la RRS puede ser reconocida por una recombinasa.This is concerned with targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods for producing and using such TI host cells. Claim 1: A targeted integration (TI) host cell comprising an exogenous nucleotide sequence integrated into an integration site within a specific locus of the host cell genome, wherein the locus is at least about 90% homologous to a sequence selected from sequences with identification numbers: 1-7. Claim 13: The TI host cell of any of claims 1-12, wherein the exogenous nucleotide sequence comprises one or more recombination recognition sequences (RRS) , where the RRS can be recognized by a recombinase.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762609806P | 2017-12-22 | 2017-12-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR113432A1 true AR113432A1 (en) | 2020-04-29 |
Family
ID=70465601
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP180103814A AR113432A1 (en) | 2017-12-22 | 2018-12-21 | TARGETED INTEGRATION OF NUCLEIC ACIDS |
Country Status (1)
Country | Link |
---|---|
AR (1) | AR113432A1 (en) |
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2018
- 2018-12-21 AR ARP180103814A patent/AR113432A1/en unknown
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