AR113432A1 - TARGETED INTEGRATION OF NUCLEIC ACIDS - Google Patents

TARGETED INTEGRATION OF NUCLEIC ACIDS

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Publication number
AR113432A1
AR113432A1 ARP180103814A ARP180103814A AR113432A1 AR 113432 A1 AR113432 A1 AR 113432A1 AR P180103814 A ARP180103814 A AR P180103814A AR P180103814 A ARP180103814 A AR P180103814A AR 113432 A1 AR113432 A1 AR 113432A1
Authority
AR
Argentina
Prior art keywords
targeted integration
host cell
nucleic acids
rrs
nucleotide sequence
Prior art date
Application number
ARP180103814A
Other languages
Spanish (es)
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of AR113432A1 publication Critical patent/AR113432A1/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente se refiere a las células huésped de integración dirigida (TI) adecuadas para la expresión de proteínas recombinantes, así como también a los métodos para producir y usar dichas células huésped de TI. Reivindicación 1: Una célula huésped de integración dirigida (TI) que comprende una secuencia de nucleótidos exógena integrada a un sitio de integración dentro de un locus específico del genoma de la célula huésped, en donde el locus es al menos aproximadamente 90% homólogo a una secuencia seleccionada de las secuencias con números de identificación: 1 - 7. Reivindicación 13: La célula huésped de TI de cualquiera de las reivindicaciones 1 - 12, en donde la secuencia de nucleótidos exógena comprende una o más secuencias de reconocimiento de recombinación (RRS), en donde la RRS puede ser reconocida por una recombinasa.This is concerned with targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods for producing and using such TI host cells. Claim 1: A targeted integration (TI) host cell comprising an exogenous nucleotide sequence integrated into an integration site within a specific locus of the host cell genome, wherein the locus is at least about 90% homologous to a sequence selected from sequences with identification numbers: 1-7. Claim 13: The TI host cell of any of claims 1-12, wherein the exogenous nucleotide sequence comprises one or more recombination recognition sequences (RRS) , where the RRS can be recognized by a recombinase.

ARP180103814A 2017-12-22 2018-12-21 TARGETED INTEGRATION OF NUCLEIC ACIDS AR113432A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762609806P 2017-12-22 2017-12-22

Publications (1)

Publication Number Publication Date
AR113432A1 true AR113432A1 (en) 2020-04-29

Family

ID=70465601

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180103814A AR113432A1 (en) 2017-12-22 2018-12-21 TARGETED INTEGRATION OF NUCLEIC ACIDS

Country Status (1)

Country Link
AR (1) AR113432A1 (en)

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