AR111761A1 - A METHOD FOR PURIFYING AN ANTIBODY OR AN ANTIBODY FRAGMENT FROM THIS USING AFFINITY CHROMATOGRAPHY - Google Patents

A METHOD FOR PURIFYING AN ANTIBODY OR AN ANTIBODY FRAGMENT FROM THIS USING AFFINITY CHROMATOGRAPHY

Info

Publication number
AR111761A1
AR111761A1 ARP180101273A ARP180101273A AR111761A1 AR 111761 A1 AR111761 A1 AR 111761A1 AR P180101273 A ARP180101273 A AR P180101273A AR P180101273 A ARP180101273 A AR P180101273A AR 111761 A1 AR111761 A1 AR 111761A1
Authority
AR
Argentina
Prior art keywords
antibody
antibody fragment
affinity chromatography
purifying
fragment
Prior art date
Application number
ARP180101273A
Other languages
Spanish (es)
Inventor
Gyong Ha
- Lee Dong Eok Sik
Yoo Hee Yang
Chung Min Lee
Se Jun Kim
Hong Jai Lee
Jung Min Yoo
Sung Kang
- Kim Kyunghwa Muk
Original Assignee
Cj Healthcare Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Healthcare Corp filed Critical Cj Healthcare Corp
Publication of AR111761A1 publication Critical patent/AR111761A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Abstract

En forma específica, a un método para separar el anticuerpo y fragmento de anticuerpo de este con alta pureza y purificar el mismo usando un buffer de elución capaz de aumentar la estabilidad para la mejora de la estabilidad. Reivindicación 1: Un método para purificar un anticuerpo blanco o un fragmento de anticuerpo de este, que comprende: (a) cargar una muestra que comprende un anticuerpo o un fragmento de anticuerpo de este en una columna de cromatografía de afinidad que comprende una resina de afinidad de cadena pesada; (b) lavar la columna con un buffer de lavado; y (c) recuperar el anticuerpo blanco o el fragmento de anticuerpo de este de la columna de cromatografía de afinidad usando un buffer de elución, que tiene un pH que varía de pH 3,8 a pH 4,7.Specifically, to a method to separate the antibody and antibody fragment thereof with high purity and purify it using an elution buffer capable of increasing stability for the improvement of stability. Claim 1: A method for purifying a white antibody or an antibody fragment thereof, comprising: (a) loading a sample comprising an antibody or an antibody fragment thereof into an affinity chromatography column comprising a resin of heavy chain affinity; (b) wash the column with a wash buffer; and (c) recovering the white antibody or the antibody fragment thereof from the affinity chromatography column using an elution buffer, which has a pH ranging from pH 3.8 to pH 4.7.

ARP180101273A 2017-05-16 2018-05-15 A METHOD FOR PURIFYING AN ANTIBODY OR AN ANTIBODY FRAGMENT FROM THIS USING AFFINITY CHROMATOGRAPHY AR111761A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20170060535 2017-05-16

Publications (1)

Publication Number Publication Date
AR111761A1 true AR111761A1 (en) 2019-08-14

Family

ID=64603003

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180101273A AR111761A1 (en) 2017-05-16 2018-05-15 A METHOD FOR PURIFYING AN ANTIBODY OR AN ANTIBODY FRAGMENT FROM THIS USING AFFINITY CHROMATOGRAPHY

Country Status (3)

Country Link
KR (1) KR102087823B1 (en)
AR (1) AR111761A1 (en)
TW (1) TWI679052B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102209790B1 (en) * 2018-12-20 2021-02-01 에이치케이이노엔 주식회사 Method for purification of vaccine virus using affinity chromatography

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG2013061528A (en) * 2008-08-14 2015-03-30 Merck Sharp & Dohme Methods for purifying antibodies using protein a affinity chromatography
AR096713A1 (en) * 2013-06-25 2016-01-27 Cadila Healthcare Ltd PURIFICATION PROCESS FOR MONOCLONAL ANTIBODIES
RU2017120358A (en) * 2014-11-10 2018-12-13 Ф.Хоффманн-Ля Рош Аг ANTI-IL-1-BETA ANTIBODIES AND WAYS OF THEIR APPLICATION

Also Published As

Publication number Publication date
TW201900257A (en) 2019-01-01
KR20180125899A (en) 2018-11-26
TWI679052B (en) 2019-12-11
KR102087823B1 (en) 2020-03-13

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