AR107210A2 - AN ISOLATED MOLECULE OF DNA POTENTIATING FOR THE EXPRESSION OF A CODING SEQUENCE, FRAGMENT, GENETIC VARIANTE, CASSETTE, VECTOR, CELL, PLANT AND SEED CONTAINING THE MOLECULE - Google Patents

AN ISOLATED MOLECULE OF DNA POTENTIATING FOR THE EXPRESSION OF A CODING SEQUENCE, FRAGMENT, GENETIC VARIANTE, CASSETTE, VECTOR, CELL, PLANT AND SEED CONTAINING THE MOLECULE

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Publication number
AR107210A2
AR107210A2 ARP150104192A ARP150104192A AR107210A2 AR 107210 A2 AR107210 A2 AR 107210A2 AR P150104192 A ARP150104192 A AR P150104192A AR P150104192 A ARP150104192 A AR P150104192A AR 107210 A2 AR107210 A2 AR 107210A2
Authority
AR
Argentina
Prior art keywords
molecule
expression
plant
cell
coding sequence
Prior art date
Application number
ARP150104192A
Other languages
Spanish (es)
Original Assignee
Bioceres S A
Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet
Univ Nacional Del Litoral
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioceres S A, Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet, Univ Nacional Del Litoral filed Critical Bioceres S A
Priority to ARP150104192A priority Critical patent/AR107210A2/en
Publication of AR107210A2 publication Critical patent/AR107210A2/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Una molécula aislada de ADN potenciadora de la expresión de una secuencia codificante, fragmento, variante genética, casete, vector, célula, planta y semilla que contengan la molécula, en donde la molécula comprende intrones capaces de inducir un incremento en la expresión de un transgen, que pueden ser útiles en construcciones de ADN destinadas a la transformación de células vegetales, donde la célula o planta tienen integrado a su genoma una construcción que contiene uno de estos intrones bajo el control de un promotor y corriente arriba de una secuencia codificante presentando niveles de expresión superiores respecto de células o plantas transformadas con construcciones que carecen del intrón de la presente, en donde las secuencias de las regiones 5’ no codificantes de los genes antes nombrados contienen, además de los intrones, promotores de uso en construcciones de ADN y secuencias exónicas que muestran sinergismo con el efecto producido por el intrón, y donde las secuencias 5’ no codificantes de los genes COX5c a las cuales se les ha delecionado el intrón promueven la expresión tejido específica en polen cuando forman parte de construcciones de ADN para transformación vegetal.An isolated DNA molecule that enhances the expression of a coding sequence, fragment, genetic variant, cassette, vector, cell, plant and seed containing the molecule, wherein the molecule comprises introns capable of inducing an increase in the expression of a transgene , which can be useful in DNA constructs destined to the transformation of plant cells, where the cell or plant has a construction that contains one of these introns integrated into its genome under the control of a promoter and upstream of a coding sequence presenting levels of superior expression with respect to cells or plants transformed with constructs that lack the intron of the present, wherein the sequences of the 5 'non-coding regions of the aforementioned genes contain, in addition to the introns, promoters of use in DNA constructs and exonic sequences that show synergism with the effect produced by intron, and where 5 ’non-coding sequences of the COX5c genes to which the intron has been deleted promote specific tissue expression in pollen when they are part of DNA constructs for plant transformation.

ARP150104192A 2015-12-18 2015-12-18 AN ISOLATED MOLECULE OF DNA POTENTIATING FOR THE EXPRESSION OF A CODING SEQUENCE, FRAGMENT, GENETIC VARIANTE, CASSETTE, VECTOR, CELL, PLANT AND SEED CONTAINING THE MOLECULE AR107210A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ARP150104192A AR107210A2 (en) 2015-12-18 2015-12-18 AN ISOLATED MOLECULE OF DNA POTENTIATING FOR THE EXPRESSION OF A CODING SEQUENCE, FRAGMENT, GENETIC VARIANTE, CASSETTE, VECTOR, CELL, PLANT AND SEED CONTAINING THE MOLECULE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP150104192A AR107210A2 (en) 2015-12-18 2015-12-18 AN ISOLATED MOLECULE OF DNA POTENTIATING FOR THE EXPRESSION OF A CODING SEQUENCE, FRAGMENT, GENETIC VARIANTE, CASSETTE, VECTOR, CELL, PLANT AND SEED CONTAINING THE MOLECULE

Publications (1)

Publication Number Publication Date
AR107210A2 true AR107210A2 (en) 2018-04-11

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Family Applications (1)

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ARP150104192A AR107210A2 (en) 2015-12-18 2015-12-18 AN ISOLATED MOLECULE OF DNA POTENTIATING FOR THE EXPRESSION OF A CODING SEQUENCE, FRAGMENT, GENETIC VARIANTE, CASSETTE, VECTOR, CELL, PLANT AND SEED CONTAINING THE MOLECULE

Country Status (1)

Country Link
AR (1) AR107210A2 (en)

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