AR107175A1 - A METHOD FOR IDENTIFYING A STRATEGY TO ACHIEVE FERTILIZATION IN MAMMALS AND THE RIGHT TIME FOR INSEMINATION - Google Patents
A METHOD FOR IDENTIFYING A STRATEGY TO ACHIEVE FERTILIZATION IN MAMMALS AND THE RIGHT TIME FOR INSEMINATIONInfo
- Publication number
- AR107175A1 AR107175A1 ARP160103995A ARP160103995A AR107175A1 AR 107175 A1 AR107175 A1 AR 107175A1 AR P160103995 A ARP160103995 A AR P160103995A AR P160103995 A ARP160103995 A AR P160103995A AR 107175 A1 AR107175 A1 AR 107175A1
- Authority
- AR
- Argentina
- Prior art keywords
- patterns
- apm
- location patterns
- location
- percentage
- Prior art date
Links
Abstract
Reivindicación 1: Un método para identificar una estrategia para alcanzar la fertilización en mamíferos que comprende los siguientes pasos: tratar una primera muestra de células espermáticas capacitadas in vitro con un marcador fluorescente; obtener una o más imágenes fluorescentes t₀ que exhiben uno o más patrones de localización de GM₁ asociados con células espermáticas capacitadas in vitro con marcado fluorescente t₀, dichas imágenes fluorescentes t₀ se obtienen en momentos post-capacitación in vitro seleccionados entre: 0,1 hora a 5 horas; 0,1 hora a 8 horas; 0,1 a 12 horas; o 0,1 hora a 18 horas (t₀); medir una cantidad de patrones de localización de GM₁ en el acrosoma apical (AA), una cantidad de patrones de localización de GM₁ en la membrana plasmática acrosomal (APM) y una cantidad total de patrones de localización de GM₁ para las células espermáticas capacitadas in vitro con marcador fluorescente t₀ exhibidas en las imágenes con fluorescencia t₀ para determinar un porcentaje de [patrones de localización GM₁ AA más patrones de localización GM₁ APM] t₀; determinar un estado de fertilidad asociado con un porcentaje de [patrones de localización de GM₁ AA más patrones de localización de GM₁ APM] t₀ donde un porcentaje de referencia de [patrones de localización de GM₁ AA más patrones de localización de GM₁ APM] correspondiente a: mayor que 35% indica un estado de fertilidad alta; un desvío estándar debajo de 35% indica un estado de fertilidad media; y dos o más desvíos estándar debajo de 35% indican un estado de fertilidad baja; comparar el porcentaje de [patrones de localización de GM₁ AA y patrones de localización de GM₁ APM] t₀ medido con el porcentaje de referencia de [patrones de localización de GM₁ AA más patrones de localización de GM₁ APM]; y identificar una estrategia reproductiva sobre la base del estado de la fertilidad a fin de alcanzar la fertilización.Claim 1: A method for identifying a strategy to achieve mammalian fertilization comprising the following steps: treating a first sample of sperm cells trained in vitro with a fluorescent marker; obtaining one or more t₀ fluorescent images that exhibit one or more GM₁ localization patterns associated with in vitro trained sperm cells with t₀ fluorescent labeling, said t₀ fluorescent images are obtained at post-in vitro training moments selected from: 0.1 hour to 5 hours; 0.1 hour to 8 hours; 0.1 to 12 hours; or 0.1 hour to 18 hours (t₀); measure a number of GM₁ localization patterns in the apical acrosome (AA), a quantity of GM₁ localization patterns in the acrosomal plasma membrane (APM) and a total amount of GM₁ localization patterns for in vitro trained sperm cells with fluorescent marker t₀ displayed on images with fluorescence t₀ to determine a percentage of [GM₁ AA location patterns plus GM₁ APM location patterns] t₀; determine a fertility status associated with a percentage of [GM₁ AA location patterns plus GM₁ APM location patterns] t₀ where a reference percentage of [GM₁ AA location patterns plus GM₁ APM location patterns] corresponding to: greater than 35% indicates a high fertility status; a standard deviation below 35% indicates a state of medium fertility; and two or more standard deviations below 35% indicate a low fertility status; compare the percentage of [GM₁ AA location patterns and GM₁ APM location patterns] t₀ measured with the reference percentage of [GM₁ AA location patterns plus GM₁ APM location patterns]; and identify a reproductive strategy based on the state of fertility in order to achieve fertilization.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562387348P | 2015-12-23 | 2015-12-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR107175A1 true AR107175A1 (en) | 2018-03-28 |
Family
ID=61768638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP160103995A AR107175A1 (en) | 2015-12-23 | 2016-12-22 | A METHOD FOR IDENTIFYING A STRATEGY TO ACHIEVE FERTILIZATION IN MAMMALS AND THE RIGHT TIME FOR INSEMINATION |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR107175A1 (en) |
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2016
- 2016-12-22 AR ARP160103995A patent/AR107175A1/en unknown
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