AR107122A1 - PESTICIDE GENES AND METHODS OF USE - Google Patents

PESTICIDE GENES AND METHODS OF USE

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Publication number
AR107122A1
AR107122A1 ARP160103974A ARP160103974A AR107122A1 AR 107122 A1 AR107122 A1 AR 107122A1 AR P160103974 A ARP160103974 A AR P160103974A AR P160103974 A ARP160103974 A AR P160103974A AR 107122 A1 AR107122 A1 AR 107122A1
Authority
AR
Argentina
Prior art keywords
polypeptides
methods
amino acid
polypeptide
recombinant
Prior art date
Application number
ARP160103974A
Other languages
Spanish (es)
Original Assignee
Agbiome Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agbiome Inc filed Critical Agbiome Inc
Publication of AR107122A1 publication Critical patent/AR107122A1/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proveen composiciones con actividad pesticida y métodos de uso de las mismas. Las composiciones incluyen polipéptidos aislados y recombinantes que poseen actividad pesticida, moléculas de ácidos nucleicos recombinantes y sintéticos que codifican los polipéptidos, construcciones de ADN y vectores que comprenden las moléculas de ácido nucleico, células huésped que comprenden los vectores, y anticuerpos para los polipéptidos. Las secuencias de polinucleótidos que codifican los polipéptidos se pueden utilizar en construcciones de ADN o casetes de expresión para la transformación y expresión en organismos de interés. Las composiciones y métodos provistos son útiles para la producción de organismos con una mejor resistencia o tolerancia a las plagas. También se proporcionan plantas y semillas transgénicas que comprenden una secuencia de nucleótidos que codifica una proteína pesticida. Tales plantas son resistentes a los insectos y otras plagas. Se proveen métodos para la producción de los diversos polipéptidos descriptos en la presente, y para el uso de esos polipéptidos en el control o eliminación de una plaga. También se incluyen métodos y kits para la detección de polipéptidos en una muestra. Reivindicación 1: Un polipéptido recombinante que posee actividad pesticida, caracterizado porque comprende: (a) un polipéptido que comprende una secuencia de aminoácidos que posee por lo menos 90% de identidad de secuencia respecto de una secuencia de aminoácidos seleccionada del grupo integrado por las secuencias de acuerdo a las Ids. de Sec. Nº 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 18, 52, 151, 152, 174, 54, 149, 181, 56, 23, 29, 154, 26, 25, 65, 146, 20, 57, 48, 63, 145, 2, 3, 7, 102, 80, 131, 105, 165, 9, 82, 11, 197, 50, 83, 14, 187, 128, 13, 107, 99, 100, 16, 143, 126, 58, 87, 31, 32, 121, 123, 124, 122, 119, 201, 202, 111, 110, 34, 90, 98, 61, 97, 76, 194, 37, 138, 114, 168, 36, 37, 136, 73, 74, 96, 132, 67, 176, 46, 95, 163, 171, 173, 71, 72, 204, 133, 134, 39, 40, 160 o 59; o (b) un polipéptido que comprende la secuencia de aminoácidos de acuerdo a las Ids. de Sec. Nº 205, 206, 207, 208, 209, 210 211, 212, 213, 214, 18, 52, 151, 152, 174, 54, 149, 181, 56, 23, 29, 154, 26, 25, 65, 146, 20, 57, 48, 63, 145, 2, 3, 7, 102, 80, 131, 105, 165, 9, 82, 11, 197, 50, 83, 14, 187, 128, 13, 107, 99, 100, 16, 143, 126, 58, 87, 31, 32, 121, 123, 124, 122, 119, 201, 202, 111, 110, 34, 90, 98, 61, 97, 76, 194, 137, 138, 114, 168, 36, 37, 136, 73, 74, 96, 132, 67, 176, 46, 95, 163, 171, 173, 71, 72, 204, 133, 134, 39, 40, 160 o 59.Compositions with pesticidal activity and methods of use thereof are provided. The compositions include isolated and recombinant polypeptides that possess pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising nucleic acid molecules, host cells comprising vectors, and antibodies to the polypeptides. The polynucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for the production of organisms with a better resistance or tolerance to pests. Transgenic plants and seeds that comprise a nucleotide sequence encoding a pesticidal protein are also provided. Such plants are resistant to insects and other pests. Methods are provided for the production of the various polypeptides described herein, and for the use of those polypeptides in the control or elimination of a pest. Methods and kits for the detection of polypeptides in a sample are also included. Claim 1: A recombinant polypeptide having pesticidal activity, characterized in that it comprises: (a) a polypeptide comprising an amino acid sequence that has at least 90% sequence identity with respect to an amino acid sequence selected from the group consisting of the sequences according to ID. of Sec. No. 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 18, 52, 151, 152, 174, 54, 149, 181, 56, 23, 29, 154, 26, 25, 65, 146, 20, 57, 48, 63, 145, 2, 3, 7, 102, 80, 131, 105, 165, 9, 82, 11, 197, 50, 83, 14, 187, 128, 13, 107, 99, 100, 16, 143, 126, 58, 87, 31, 32, 121, 123, 124, 122, 119, 201, 202, 111, 110, 34, 90, 98, 61, 97, 76, 194, 37, 138, 114, 168, 36, 37, 136, 73, 74, 96, 132, 67, 176, 46, 95, 163, 171, 173, 71, 72, 204, 133, 134, 39, 40, 160 or 59; or (b) a polypeptide comprising the amino acid sequence according to ID. of Sec. No. 205, 206, 207, 208, 209, 210 211, 212, 213, 214, 18, 52, 151, 152, 174, 54, 149, 181, 56, 23, 29, 154, 26, 25 , 65, 146, 20, 57, 48, 63, 145, 2, 3, 7, 102, 80, 131, 105, 165, 9, 82, 11, 197, 50, 83, 14, 187, 128, 13 , 107, 99, 100, 16, 143, 126, 58, 87, 31, 32, 121, 123, 124, 122, 119, 201, 202, 111, 110, 34, 90, 98, 61, 97, 76 , 194, 137, 138, 114, 168, 36, 37, 136, 73, 74, 96, 132, 67, 176, 46, 95, 163, 171, 173, 71, 72, 204, 133, 134, 39 , 40, 160 or 59.

ARP160103974A 2015-12-22 2016-12-22 PESTICIDE GENES AND METHODS OF USE AR107122A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562270742P 2015-12-22 2015-12-22

Publications (1)

Publication Number Publication Date
AR107122A1 true AR107122A1 (en) 2018-03-21

Family

ID=61768606

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160103974A AR107122A1 (en) 2015-12-22 2016-12-22 PESTICIDE GENES AND METHODS OF USE

Country Status (1)

Country Link
AR (1) AR107122A1 (en)

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