AR104956A1 - IMPROVED METHOD FOR THE PRODUCTION OF G-CARBOXYLED POLYPEPTIDES - Google Patents

IMPROVED METHOD FOR THE PRODUCTION OF G-CARBOXYLED POLYPEPTIDES

Info

Publication number
AR104956A1
AR104956A1 ARP160101723A ARP160101723A AR104956A1 AR 104956 A1 AR104956 A1 AR 104956A1 AR P160101723 A ARP160101723 A AR P160101723A AR P160101723 A ARP160101723 A AR P160101723A AR 104956 A1 AR104956 A1 AR 104956A1
Authority
AR
Argentina
Prior art keywords
production
carboxyled
polypeptides
improved method
polypeptide
Prior art date
Application number
ARP160101723A
Other languages
Spanish (es)
Inventor
Jahn Doreen
Stahn Rainer
Dr Goletz Steffen
Original Assignee
Glycotope Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycotope Gmbh filed Critical Glycotope Gmbh
Publication of AR104956A1 publication Critical patent/AR104956A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Métodos para producir un polipéptido g-carboxilado en una célula huésped que presenta actividad de g-glutamil carboxilasa. La producción varía merced a la presencia de vitamina K y ciclodextrina en el medio que se usa para cultivar las células. Reivindicación 1: Un método para producir un polipéptido g-carboxilado, caracterizado porque comprende cultivar una célula huésped que presenta actividad de g-glutamil carboxilasa y que comprende una secuencia de nucleótidos que codifica el polipéptido correspondiente en un medio de cultivo apropiado, donde el medio de cultivo comprende vitamina K y ciclodextrina.Methods for producing a g-carboxylated polypeptide in a host cell that exhibits g-glutamyl carboxylase activity. Production varies thanks to the presence of vitamin K and cyclodextrin in the medium used to grow the cells. Claim 1: A method of producing a g-carboxylated polypeptide, characterized in that it comprises culturing a host cell that exhibits g-glutamyl carboxylase activity and comprising a nucleotide sequence encoding the corresponding polypeptide in an appropriate culture medium, wherein the medium of culture comprises vitamin K and cyclodextrin.

ARP160101723A 2015-06-09 2016-06-09 IMPROVED METHOD FOR THE PRODUCTION OF G-CARBOXYLED POLYPEPTIDES AR104956A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LU92735 2015-06-09

Publications (1)

Publication Number Publication Date
AR104956A1 true AR104956A1 (en) 2017-08-30

Family

ID=53499060

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160101723A AR104956A1 (en) 2015-06-09 2016-06-09 IMPROVED METHOD FOR THE PRODUCTION OF G-CARBOXYLED POLYPEPTIDES

Country Status (2)

Country Link
AR (1) AR104956A1 (en)
WO (1) WO2016198499A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7572619B2 (en) 2000-03-22 2009-08-11 Octagene Gmbh Recombinant blood clotting factors
WO2003048313A2 (en) * 2001-11-30 2003-06-12 Invitrogen Corporation Cell culture media
KR101603632B1 (en) 2006-09-10 2016-03-16 글리코토페 게엠베하 Use of human cells of myeloid leukaemia origin for expression of antibodies
US8999962B2 (en) * 2008-06-03 2015-04-07 John Ray Biffin Method for increasing bone density and/or reducing any osteochondral defects in an animal and a composition including vitamin K

Also Published As

Publication number Publication date
WO2016198499A1 (en) 2016-12-15

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