AR098754A1 - ENZYMATIC HYDROLYSIS PROCEDURE WITH IN-SITU PRODUCTION OF HYDROLASE GLUCOSIDS BY GENETICALLY MODIFIED MICROORGANISMS (MGM) AND NOT MGM - Google Patents

ENZYMATIC HYDROLYSIS PROCEDURE WITH IN-SITU PRODUCTION OF HYDROLASE GLUCOSIDS BY GENETICALLY MODIFIED MICROORGANISMS (MGM) AND NOT MGM

Info

Publication number
AR098754A1
AR098754A1 ARP140104658A ARP140104658A AR098754A1 AR 098754 A1 AR098754 A1 AR 098754A1 AR P140104658 A ARP140104658 A AR P140104658A AR P140104658 A ARP140104658 A AR P140104658A AR 098754 A1 AR098754 A1 AR 098754A1
Authority
AR
Argentina
Prior art keywords
mgm
enzymatic hydrolysis
genetically modified
enzymes
glucosids
Prior art date
Application number
ARP140104658A
Other languages
Spanish (es)
Inventor
Ben Chaabane Fadhel
Guy Berrin Jean
Original Assignee
Ifp Energies Now
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ifp Energies Now filed Critical Ifp Energies Now
Publication of AR098754A1 publication Critical patent/AR098754A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un procedimiento de hidrólisis enzimática de materiales lignocelulósicos, en el que el medio de reacción producido in situ comprende una mezcla de hongo Trichoderma reesei no modificado genéticamente (no MGM) y las enzimas que secreta, completado con enzimas purificadas secretadas por al menos un microorganismo modificado genéticamente (MGM), que las citadas enzimas han sido separadas del citado microorganismo MGM, y la proporción de las citadas enzimas secretadas por el microorganismo MGM y separadas del citado microorganismo es de 1% a 20% con respecto a la cantidad total de enzimas.An enzymatic hydrolysis process of lignocellulosic materials, in which the reaction medium produced in situ comprises a mixture of genetically modified Trichoderma reesei (non-MGM) fungus and the enzymes it secretes, completed with purified enzymes secreted by at least one modified microorganism genetically (MGM), that the aforementioned enzymes have been separated from said MGM microorganism, and the proportion of said enzymes secreted by the MGM microorganism and separated from said microorganism is from 1% to 20% with respect to the total amount of enzymes.

ARP140104658A 2013-12-17 2014-12-12 ENZYMATIC HYDROLYSIS PROCEDURE WITH IN-SITU PRODUCTION OF HYDROLASE GLUCOSIDS BY GENETICALLY MODIFIED MICROORGANISMS (MGM) AND NOT MGM AR098754A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1362831A FR3014903B1 (en) 2013-12-17 2013-12-17 ENZYMATIC HYDROLYSIS METHOD WITH IN SITU PRODUCTION OF HYDROLASED GLYCOSIDES BY GENETICALLY MODIFIED MICROORGANISMS (MGM) AND NON-MGM

Publications (1)

Publication Number Publication Date
AR098754A1 true AR098754A1 (en) 2016-06-08

Family

ID=50473461

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140104658A AR098754A1 (en) 2013-12-17 2014-12-12 ENZYMATIC HYDROLYSIS PROCEDURE WITH IN-SITU PRODUCTION OF HYDROLASE GLUCOSIDS BY GENETICALLY MODIFIED MICROORGANISMS (MGM) AND NOT MGM

Country Status (3)

Country Link
AR (1) AR098754A1 (en)
FR (1) FR3014903B1 (en)
WO (1) WO2015091079A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3049957B1 (en) * 2016-04-08 2020-09-25 Ifp Energies Now PROCESS FOR THE PRODUCTION OF CELLULASES WITH PRETREATED LIGNOCELLULOSIC MARK
FR3113291A1 (en) 2020-08-06 2022-02-11 IFP Energies Nouvelles Process for the production of alcohol by enzymatic hydrolysis and fermentation of lignocellulosic biomass

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3990945A (en) 1975-04-28 1976-11-09 Bio Research Center Company Limited Enzymatic hydrolysis of cellulose
CH663113A5 (en) 1983-11-28 1987-11-13 Sprecher & Schuh Ag COUPLING ARRANGEMENT BETWEEN AN ELECTROMAGNETIC SWITCHING DEVICE AND A REMOVABLE AUXILIARY CONTACT BLOCK.
CA2698765A1 (en) * 2007-09-07 2009-03-19 Danisco Us Inc. Beta-glucosidase enhanced filamentous fungal whole cellulase compositions and methods of use
DK2268804T3 (en) * 2008-03-21 2017-12-11 Danisco Us Inc HEMICELLULASE-ENRICHED COMPOSITIONS FOR IMPROVED BIOMASS HYDROLYSE
FR2935986B1 (en) 2008-09-12 2012-11-23 Inst Francais Du Petrole BETA-GLUCOSIDASE VARIANTS WITH IMPROVED ACTIVITY AND USES THEREOF
RU2013146245A (en) * 2011-03-17 2015-04-27 ДАНИСКО ЮЭс ИНК. METHOD FOR REDUCING VISCOSITY IN THE SUGARING PROCESS
SG192025A1 (en) * 2011-03-17 2013-08-30 Danisco Us Inc Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
US20140073017A1 (en) * 2011-03-17 2014-03-13 Danisco Us Inc. Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars

Also Published As

Publication number Publication date
FR3014903B1 (en) 2017-12-01
FR3014903A1 (en) 2015-06-19
WO2015091079A1 (en) 2015-06-25

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