AR087037A1 - AMILOID FIBERS OF LISOZIMA, SOLID SUPPORT, BIOCATALIZER AND PROCEDURES - Google Patents

AMILOID FIBERS OF LISOZIMA, SOLID SUPPORT, BIOCATALIZER AND PROCEDURES

Info

Publication number
AR087037A1
AR087037A1 ARP120102400A ARP120102400A AR087037A1 AR 087037 A1 AR087037 A1 AR 087037A1 AR P120102400 A ARP120102400 A AR P120102400A AR P120102400 A ARP120102400 A AR P120102400A AR 087037 A1 AR087037 A1 AR 087037A1
Authority
AR
Argentina
Prior art keywords
lysozyme
fibers
biological
biocatalizer
lisozima
Prior art date
Application number
ARP120102400A
Other languages
Spanish (es)
Inventor
Nieves Chehin Rosana
Silvina Chaves Analia
Dario Borsarelli Claudio
Mariana Romero Cintia
Maria Pera Licia
Domingo Baigori Mario
Original Assignee
Consejo Nac Invest Cient Tec
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Nac Invest Cient Tec filed Critical Consejo Nac Invest Cient Tec
Priority to ARP120102400A priority Critical patent/AR087037A1/en
Priority to PCT/IB2013/055405 priority patent/WO2014006560A2/en
Priority to BR112015000022A priority patent/BR112015000022A2/en
Publication of AR087037A1 publication Critical patent/AR087037A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Fibras amiloide de lisozima, en donde la lisozima está autoensamblada formando complejos fibrosos (nanotubos biológicos) cuya estructura secundaria comprende al menos una estructura cross-b, siendo que la lisozima nativa carece de dichas estructuras. Las fibras amiloides de lisozima carecen de actividad biológica y enzimática. El soporte para la unión de proteínas comprende a las fibras amiloides de lisozima y el biocatalizador comprende al menos una enzima unida al soporte de fibras amiloides de lisozima por un método de fotosensibilización. La proteína inmovilizada puede ser una enzima, por ejemplo una lipasa. El procedimiento incrementa notablemente la estabilidad enzimática del producto biológico inmovilizado.Lysozyme amyloid fibers, where lysozyme is self-assembled forming fibrous complexes (biological nanotubes) whose secondary structure comprises at least one cross-b structure, with the native lysozyme lacking such structures. Lysozyme amyloid fibers lack biological and enzymatic activity. The support for protein binding comprises the lysozyme amyloid fibers and the biocatalyst comprises at least one enzyme linked to the lysozyme amyloid fiber support by a photosensitization method. The immobilized protein can be an enzyme, for example a lipase. The process significantly increases the enzymatic stability of the immobilized biological product.

ARP120102400A 2012-07-02 2012-07-02 AMILOID FIBERS OF LISOZIMA, SOLID SUPPORT, BIOCATALIZER AND PROCEDURES AR087037A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ARP120102400A AR087037A1 (en) 2012-07-02 2012-07-02 AMILOID FIBERS OF LISOZIMA, SOLID SUPPORT, BIOCATALIZER AND PROCEDURES
PCT/IB2013/055405 WO2014006560A2 (en) 2012-07-02 2013-07-01 Lysozyme amyloid fibers, solid carrier, biocatalyst and processes
BR112015000022A BR112015000022A2 (en) 2012-07-02 2013-07-01 lysozyme amyloid fiber, protein immobilization support, biocatalyst, process for preparing a support, process for producing a biocatalyst, fiber use and support use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP120102400A AR087037A1 (en) 2012-07-02 2012-07-02 AMILOID FIBERS OF LISOZIMA, SOLID SUPPORT, BIOCATALIZER AND PROCEDURES

Publications (1)

Publication Number Publication Date
AR087037A1 true AR087037A1 (en) 2014-02-12

Family

ID=49882538

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120102400A AR087037A1 (en) 2012-07-02 2012-07-02 AMILOID FIBERS OF LISOZIMA, SOLID SUPPORT, BIOCATALIZER AND PROCEDURES

Country Status (3)

Country Link
AR (1) AR087037A1 (en)
BR (1) BR112015000022A2 (en)
WO (1) WO2014006560A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110904080A (en) * 2019-12-10 2020-03-24 陕西理工大学 Induction method of lysozyme amyloid fiber model

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7166451B1 (en) * 2003-02-24 2007-01-23 The Ohio State University Immobilization of enzyme on a fibrous matrix
EP1991662B1 (en) * 2006-02-21 2018-02-07 The Trustees Of Princeton University High-yield activation of polymer surfaces for covalent attachment of molecules
US8591932B2 (en) * 2009-09-17 2013-11-26 W. L. Gore & Associates, Inc. Heparin entities and methods of use

Also Published As

Publication number Publication date
WO2014006560A3 (en) 2015-09-17
BR112015000022A2 (en) 2017-06-27
WO2014006560A2 (en) 2014-01-09

Similar Documents

Publication Publication Date Title
CY1122690T1 (en) PROCEDURE FOR FRIDAY BETA-3 COMPETITORS AND INTERMEDIATES
MY165630A (en) An integrated process for producing biofuels
BR112015000950A2 (en) HYDROLYSIS METHOD OF A PRETREATED CELLULOSIC MATERIAL, PRODUCTION PROCESS OF A FERMENTATION PRODUCT, AND COMPOSITION
IN2014MN00989A (en)
WO2014176531A3 (en) Co-solvent to produce reactive intermediates from biomass
EA201490895A1 (en) PRODUCTION OF SUGAR ALCOHOL FROM BIOMASS
ECSP13013114A (en) EXTRACTION OF CHITIN IN A SINGLE STAGE BY ENZYMATIC HYDROLYSIS IN THE MIDDLE ACID
WO2012019175A8 (en) Production of malonyl-coa derived products via anaerobic pathways
BR112012029999A2 (en) method for biofuel production
BR112013018857A2 (en) process for the production of fatty acid alcohol esters and composition of the alcohol fermentation process
BR112014014656A2 (en) Innovative bacterial culture process to improve capsular polyiosis productivity
PE20130048A1 (en) APPARATUS AND PROCESS FOR THE FERMENTATION OF HYDROLYZED BIOMASS
EA201400076A1 (en) METHOD FOR CREATING ORGANIC MATERIAL
BRPI0819641A2 (en) ALCOHOL PRODUCTION PROCESS IN A BIORFINERY CONTEXT
BR112016010340A2 (en) new methods for producing active tert
EA201101626A1 (en) BACTERIA WITH HIGHLY EFFICIENT METABOLISM
AR079187A1 (en) FERMENTATION PROCESS FOR THE PRODUCTION OF GLYCOLIC ACID
BR112012030528A2 (en) process and microorganisms for lipid production
BR112016023823A2 (en) method for producing a fermentation product
WO2013188313A3 (en) Fungal xylanases and xylosidases
BR112017020906A2 (en) microalgae adapted for heterotrophic culture conditions
BRPI1009574A2 (en) Method for reducing the production of co2 in a fermentation process to produce an alcohol, and modified microorganism.
BR112015029081A2 (en) MYCELIOPHTHORA THERMOPHILA HOST CELL EXPRESSING A RECOMBINANT BETA-XYLOSIDASE ENZYME, RECOMBINANT BETA-XYLOSIDASE ENZYME EXPRESSED THROUGH THE SAME, COMPOSITION, USE OF HOST CELL, BETA-XYLOSIDASE ENZYME OR COMPOSITION AND METHODS OF PRODUCING FERMENTED SUGARS AND PRODUCTION OF BIO PRODUCTS OF BIOMASS
BR112012031965A8 (en) use of inducible promoters in the production of glycolic acid
BR112016009619A2 (en) methods for saccharifying a cellulosic material and for producing a fermentation product from cellulosic material

Legal Events

Date Code Title Description
FG Grant, registration