AR086406A1 - NEMATODE RESISTANT TRANSGENIC PLANTS - Google Patents

NEMATODE RESISTANT TRANSGENIC PLANTS

Info

Publication number
AR086406A1
AR086406A1 ARP120101716A ARP120101716A AR086406A1 AR 086406 A1 AR086406 A1 AR 086406A1 AR P120101716 A ARP120101716 A AR P120101716A AR P120101716 A ARP120101716 A AR P120101716A AR 086406 A1 AR086406 A1 AR 086406A1
Authority
AR
Argentina
Prior art keywords
polynucleotide
chain
plant
expression
seq
Prior art date
Application number
ARP120101716A
Other languages
Spanish (es)
Original Assignee
Basf Plant Science Co Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Co Gmbh filed Critical Basf Plant Science Co Gmbh
Publication of AR086406A1 publication Critical patent/AR086406A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/0107—DELTA14-sterol reductase (1.3.1.70)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Composiciones de ARN bicatenarios y plantas transgénicas capaces de inhibir la expresión de genes de plantas y métodos asociados con ello. Específicamente, uso de interferencia de ARN para inhibir la expresión de un polinucleótido de esterol C-14 reductasa de planta, y generación de plantas que tienen una mayor resistencia a nematodos parásitos.Reivindicación 1: Un vector de expresión aislado caracterizado porque codifica un ARN bicatenario que comprende una primera cadena y una segunda cadena complementaria a la primera cadena, en donde la primera cadena es sustancialmente idéntica a al menos 19, 20 ó 21 nucleótidos consecutivos de un polinucleótido blanco de planta que codifica una esterol C-14 reductasa de planta seleccionada del grupo que consiste en (a) un polinucleótido que tiene una secuencia establecida en la SEQ ID Nº 1, (b) un polinucleótido que tiene una secuencia establecida en la SEQ ID Nº 4, y (c) un polinucleótido que tiene una secuencia establecida en la SEQ ID Nº 6, en donde el ARN bicatenario inhibe la expresión del polinucleótido objeto.Compositions of double stranded RNA and transgenic plants capable of inhibiting the expression of plant genes and methods associated therewith. Specifically, use of RNA interference to inhibit the expression of a plant sterol C-14 reductase polynucleotide, and generation of plants that have a greater resistance to parasitic nematodes. Claim 1: An isolated expression vector characterized in that it encodes a double stranded RNA comprising a first chain and a second chain complementary to the first chain, wherein the first chain is substantially identical to at least 19, 20 or 21 consecutive nucleotides of a white plant polynucleotide encoding a selected plant C-14 reductase sterol from the group consisting of (a) a polynucleotide having a sequence established in SEQ ID No. 1, (b) a polynucleotide having a sequence established in SEQ ID No. 4, and (c) a polynucleotide having an established sequence in SEQ ID No. 6, where double stranded RNA inhibits expression of the target polynucleotide.

ARP120101716A 2011-05-16 2012-05-15 NEMATODE RESISTANT TRANSGENIC PLANTS AR086406A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161486367P 2011-05-16 2011-05-16

Publications (1)

Publication Number Publication Date
AR086406A1 true AR086406A1 (en) 2013-12-11

Family

ID=47176365

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120101716A AR086406A1 (en) 2011-05-16 2012-05-15 NEMATODE RESISTANT TRANSGENIC PLANTS

Country Status (2)

Country Link
AR (1) AR086406A1 (en)
WO (1) WO2012156902A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0954568A1 (en) * 1996-06-21 1999-11-10 The General Hospital Corporation Plant sterol reductases and uses thereof
WO1998045457A1 (en) * 1996-12-26 1998-10-15 Monsanto Company Transgenic plants with modified sterol biosynthetic pathways
US20080193937A1 (en) * 2005-03-30 2008-08-14 Jitao Zou Identification of a Sterol Acyltransferase Gene

Also Published As

Publication number Publication date
WO2012156902A1 (en) 2012-11-22

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