AR086040A2 - A METHOD FOR REDUCING THE EXPRESSION OR ENZYMATIC ACTIVITY OF A TOBACCO POLYPEPTIDE - Google Patents

A METHOD FOR REDUCING THE EXPRESSION OR ENZYMATIC ACTIVITY OF A TOBACCO POLYPEPTIDE

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Publication number
AR086040A2
AR086040A2 ARP120101409A ARP120101409A AR086040A2 AR 086040 A2 AR086040 A2 AR 086040A2 AR P120101409 A ARP120101409 A AR P120101409A AR P120101409 A ARP120101409 A AR P120101409A AR 086040 A2 AR086040 A2 AR 086040A2
Authority
AR
Argentina
Prior art keywords
expression
enzymatic activity
induced
nucleic acid
plant cell
Prior art date
Application number
ARP120101409A
Other languages
Spanish (es)
Original Assignee
Us Smokeless Tobacco Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/934,944 external-priority patent/US7812227B2/en
Priority claimed from US10/943,507 external-priority patent/US7855318B2/en
Priority claimed from PCT/US2004/034218 external-priority patent/WO2005038018A2/en
Priority claimed from PCT/US2004/034065 external-priority patent/WO2005038033A2/en
Priority claimed from US11/110,062 external-priority patent/US7700851B2/en
Priority claimed from US11/116,881 external-priority patent/US7700834B2/en
Application filed by Us Smokeless Tobacco Co filed Critical Us Smokeless Tobacco Co
Publication of AR086040A2 publication Critical patent/AR086040A2/en

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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Reivindicación 1: Un método para reducir la expresión o actividad enzimática de un polipéptido de tabaco constitutivo, o inducido por etileno o inducido por senescencia en una célula vegetal, caracterizado porque dicho método comprende: introducir un transgén en dicha célula vegetal, donde dicho transgén codifica: (a) una molécula de ácido nucleico antisentido; (b) una molécula de ácido nucleico de ARN de cadena doble; o (c) una molécula de ácido nucleico sentido que resulta en la co-supresión; donde dicho transgén comprende una secuencia de ácidos nucleicos seleccionada del grupo que consiste en SEQ ID Nº 1, 4, 5, 7, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 367, 369, 371, 373, 375, 377, 379, 381, 383, 446, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, y 549 - 2193; donde la expresión de dicho transgén en dicha célula vegetal reduce el nivel de expresión o actividad enzimática de un polipéptido de tabaco constitutivo, o inducido por etileno o inducido por senescencia endógeno en dicha célula vegetal.Claim 1: A method for reducing the expression or enzymatic activity of a constituent tobacco polypeptide, or induced by ethylene or induced by senescence in a plant cell, characterized in that said method comprises: introducing a transgene into said plant cell, wherein said transgene encodes : (a) an antisense nucleic acid molecule; (b) a double stranded RNA nucleic acid molecule; or (c) a sense nucleic acid molecule that results in co-suppression; wherein said transgene comprises a nucleic acid sequence selected from the group consisting of SEQ ID No. 1, 4, 5, 7, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 367, 369, 371, 373, 375, 377, 379, 381, 383, 446, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, and 549-2193; wherein the expression of said transgene in said plant cell reduces the level of expression or enzymatic activity of a constituent tobacco polypeptide, or induced by ethylene or induced by endogenous senescence in said plant cell.

ARP120101409A 2004-04-29 2012-04-24 A METHOD FOR REDUCING THE EXPRESSION OR ENZYMATIC ACTIVITY OF A TOBACCO POLYPEPTIDE AR086040A2 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US56623504P 2004-04-29 2004-04-29
US60735704P 2004-09-03 2004-09-03
US10/934,944 US7812227B2 (en) 2001-11-13 2004-09-03 Cloning of cytochrome p450 genes from nicotiana
US10/943,507 US7855318B2 (en) 2001-11-13 2004-09-17 Cloning of cytochrome P450 genes from Nicotiana
PCT/US2004/034218 WO2005038018A2 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana
PCT/US2004/034065 WO2005038033A2 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana
US64676405P 2005-01-25 2005-01-25
US66509705P 2005-03-24 2005-03-24
US66545105P 2005-03-24 2005-03-24
US11/110,062 US7700851B2 (en) 2001-11-13 2005-04-19 Tobacco nicotine demethylase genomic clone and uses thereof
US11/116,881 US7700834B2 (en) 2001-11-13 2005-04-27 Nicotiana nucleic acid molecules and uses thereof

Publications (1)

Publication Number Publication Date
AR086040A2 true AR086040A2 (en) 2013-11-13

Family

ID=36838617

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP050101723A AR049265A1 (en) 2004-04-29 2005-04-29 NUCLEIC ACID MOLECULES OF NICOTIANA AND USES OF THE SAME
ARP120101409A AR086040A2 (en) 2004-04-29 2012-04-24 A METHOD FOR REDUCING THE EXPRESSION OR ENZYMATIC ACTIVITY OF A TOBACCO POLYPEPTIDE

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP050101723A AR049265A1 (en) 2004-04-29 2005-04-29 NUCLEIC ACID MOLECULES OF NICOTIANA AND USES OF THE SAME

Country Status (1)

Country Link
AR (2) AR049265A1 (en)

Also Published As

Publication number Publication date
AR049265A1 (en) 2006-07-12

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