AR080087A1 - METHOD TO PROMOTE THE DIFFERENTIATION OF HUMAN EMBRYOUS MOTHER CELLS - Google Patents
METHOD TO PROMOTE THE DIFFERENTIATION OF HUMAN EMBRYOUS MOTHER CELLSInfo
- Publication number
- AR080087A1 AR080087A1 ARP110100320A AR080087A1 AR 080087 A1 AR080087 A1 AR 080087A1 AR P110100320 A ARP110100320 A AR P110100320A AR 080087 A1 AR080087 A1 AR 080087A1
- Authority
- AR
- Argentina
- Prior art keywords
- cells
- lineage
- markers
- pancreatic
- characteristic
- Prior art date
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Métodos para promover la diferenciacion de células madre pluripotentes en células que producen insulina. Reivindicacion 1:Un método para aumentar la expresion de NGN3 y NKX6.1 en una poblacion de células que expresan marcadores característicos del linaje endocrino pancreático, el método comprende las etapas de: a) cultivar células madre pluripotentes, b) diferenciar las células madre pluripotentes en células que expresan marcadores característicos del linaje del endodermo definitivo, c) diferenciar las células característicos del linaje del que expresan marcadores endodermo pancreático, por que expresan marcadores endodermo definitivo en células característicos del linaje del medio de suplementar el medio que se usa para diferenciar las células que expresan marcadores característicos del linaje del endodermo definitivo con un compuesto seleccionado del grupo que consiste de H9, H-89, CF 109203X, HA-1004, PP2, PPI, LY 294002, Wortmannin, SB-203580, SB-202190, Tirfostina 25, Tirfostina, AG1478, Tirfostina 46, GW 5074, Kenpaullona, HNMPA, AG490, Y27632, y ML-7, y d) diferenciar las células que expresan marcadores característicos del linaje del endodermo pancreático en células que expresan marcadores característicos del linaje pancreático endocrino.Methods to promote the differentiation of pluripotent stem cells into cells that produce insulin. Claim 1: A method for increasing the expression of NGN3 and NKX6.1 in a population of cells expressing characteristic markers of the pancreatic endocrine lineage, the method comprises the steps of: a) culturing pluripotent stem cells, b) differentiating pluripotent stem cells in cells that express characteristic markers of the definitive endoderm lineage, c) differentiate the characteristic cells of the lineage from those that express pancreatic endoderm markers, because they express definitive endoderm markers in cells characteristic of the lineage of the medium to supplement the medium used to differentiate the cells expressing characteristic markers of the definitive endoderm lineage with a compound selected from the group consisting of H9, H-89, CF 109203X, HA-1004, PP2, PPI, LY 294002, Wortmannin, SB-203580, SB-202190, Tirfostina 25, Tirfostina, AG1478, Tirfostina 46, GW 5074, Kenpaullona, HNMPA, AG490, Y27632, and ML-7, and d) diferenci ar cells expressing characteristic markers of the pancreatic endoderm lineage in cells expressing characteristic markers of the endocrine pancreatic lineage.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/970,365 US9133439B2 (en) | 2009-12-23 | 2010-12-16 | Differentiation of human embryonic stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR080087A1 true AR080087A1 (en) | 2012-03-14 |
Family
ID=45841697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP110100320 AR080087A1 (en) | 2010-12-16 | 2011-01-31 | METHOD TO PROMOTE THE DIFFERENTIATION OF HUMAN EMBRYOUS MOTHER CELLS |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR080087A1 (en) |
-
2011
- 2011-01-31 AR ARP110100320 patent/AR080087A1/en unknown
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