AR059065A1 - METHODS TO MODULATE THE MANOSA CONTENT OF THE RECOMBINANT PROTEINS - Google Patents
METHODS TO MODULATE THE MANOSA CONTENT OF THE RECOMBINANT PROTEINSInfo
- Publication number
- AR059065A1 AR059065A1 ARP070100213A AR059065A1 AR 059065 A1 AR059065 A1 AR 059065A1 AR P070100213 A ARP070100213 A AR P070100213A AR 059065 A1 AR059065 A1 AR 059065A1
- Authority
- AR
- Argentina
- Prior art keywords
- recombinant
- content
- glycoprotein
- producing
- manosa
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Así también, tanto un método para producir una composicion de anticuerpo recombinante como para producir una composicion de anticuerpo recombinante monoclonal humano que enlaza la IL-15. Además composiciones de glicoproteína recombinante y anticuerpo recombinante con contenido de baja manosa. Reivindicacion 1: Un método para producir una composicion de glicoproteína recombinante con contenido de baja manosa que comprende el cultivo de una célula hospedante que expresa la glicoproteína recombinante en un medio de cultivo que tiene una osmolalidad de aproximadamente 600 mOsm/Kg o menos. Reivindicacion 37: Un método para detectar y/o cuantificar el contenido de alta manosa de una glicoproteína en una muestra, dicho método consta de someter a dicha muestra a una digestion con la enzima endoglicosidasa seguida de la separacion de las glicoproteínas digeridas mediante electroforesis.Also, both a method for producing a recombinant antibody composition and for producing a human monoclonal recombinant antibody composition that binds IL-15. In addition compositions of recombinant glycoprotein and recombinant antibody with low mannose content. Claim 1: A method of producing a low-mannose-containing recombinant glycoprotein composition comprising culturing a host cell that expresses the recombinant glycoprotein in a culture medium that has an osmolality of about 600 mOsm / Kg or less. Claim 37: A method for detecting and / or quantifying the high mannose content of a glycoprotein in a sample, said method consists of subjecting said sample to a digestion with the endoglycosidase enzyme followed by the separation of digested glycoproteins by electrophoresis.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US76147706P | 2006-01-23 | 2006-01-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR059065A1 true AR059065A1 (en) | 2008-03-12 |
Family
ID=39186214
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP070100213 AR059065A1 (en) | 2006-01-23 | 2007-01-18 | METHODS TO MODULATE THE MANOSA CONTENT OF THE RECOMBINANT PROTEINS |
Country Status (3)
Country | Link |
---|---|
CN (2) | CN111676263A (en) |
AR (1) | AR059065A1 (en) |
ZA (1) | ZA200807126B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
HU231463B1 (en) * | 2015-08-04 | 2024-01-28 | Richter Gedeon Nyrt. | Method for increasing the galactose content of recombinant proteins |
CN116590371B (en) * | 2023-07-13 | 2023-10-17 | 智享生物(苏州)有限公司 | Cell culture method for reducing high mannose type antibody in Chinese hamster ovary cells |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4925796A (en) * | 1986-03-07 | 1990-05-15 | Massachusetts Institute Of Technology | Method for enhancing glycoprotein stability |
WO2002101019A2 (en) * | 2001-06-13 | 2002-12-19 | Genentech, Inc. | Methods of culturing animal cells and polypeptide production in animal cells |
US7247304B2 (en) * | 2001-08-23 | 2007-07-24 | Genmab A/S | Methods of treating using anti-IL-15 antibodies |
-
2007
- 2007-01-18 AR ARP070100213 patent/AR059065A1/en not_active Application Discontinuation
- 2007-01-23 CN CN202010302530.7A patent/CN111676263A/en active Pending
- 2007-01-23 CN CNA2007800067616A patent/CN101410528A/en active Pending
-
2008
- 2008-08-19 ZA ZA200807126A patent/ZA200807126B/en unknown
Also Published As
Publication number | Publication date |
---|---|
CN111676263A (en) | 2020-09-18 |
ZA200807126B (en) | 2009-07-29 |
CN101410528A (en) | 2009-04-15 |
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