AR059065A1 - METHODS TO MODULATE THE MANOSA CONTENT OF THE RECOMBINANT PROTEINS - Google Patents

METHODS TO MODULATE THE MANOSA CONTENT OF THE RECOMBINANT PROTEINS

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Publication number
AR059065A1
AR059065A1 ARP070100213A AR059065A1 AR 059065 A1 AR059065 A1 AR 059065A1 AR P070100213 A ARP070100213 A AR P070100213A AR 059065 A1 AR059065 A1 AR 059065A1
Authority
AR
Argentina
Prior art keywords
recombinant
content
glycoprotein
producing
manosa
Prior art date
Application number
Other languages
Spanish (es)
Inventor
Nicole Le
La Cruz Michael De
Gregory Flynn
Jian Wu
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of AR059065A1 publication Critical patent/AR059065A1/en

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Abstract

Así también, tanto un método para producir una composicion de anticuerpo recombinante como para producir una composicion de anticuerpo recombinante monoclonal humano que enlaza la IL-15. Además composiciones de glicoproteína recombinante y anticuerpo recombinante con contenido de baja manosa. Reivindicacion 1: Un método para producir una composicion de glicoproteína recombinante con contenido de baja manosa que comprende el cultivo de una célula hospedante que expresa la glicoproteína recombinante en un medio de cultivo que tiene una osmolalidad de aproximadamente 600 mOsm/Kg o menos. Reivindicacion 37: Un método para detectar y/o cuantificar el contenido de alta manosa de una glicoproteína en una muestra, dicho método consta de someter a dicha muestra a una digestion con la enzima endoglicosidasa seguida de la separacion de las glicoproteínas digeridas mediante electroforesis.Also, both a method for producing a recombinant antibody composition and for producing a human monoclonal recombinant antibody composition that binds IL-15. In addition compositions of recombinant glycoprotein and recombinant antibody with low mannose content. Claim 1: A method of producing a low-mannose-containing recombinant glycoprotein composition comprising culturing a host cell that expresses the recombinant glycoprotein in a culture medium that has an osmolality of about 600 mOsm / Kg or less. Claim 37: A method for detecting and / or quantifying the high mannose content of a glycoprotein in a sample, said method consists of subjecting said sample to a digestion with the endoglycosidase enzyme followed by the separation of digested glycoproteins by electrophoresis.

ARP070100213 2006-01-23 2007-01-18 METHODS TO MODULATE THE MANOSA CONTENT OF THE RECOMBINANT PROTEINS AR059065A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US76147706P 2006-01-23 2006-01-23

Publications (1)

Publication Number Publication Date
AR059065A1 true AR059065A1 (en) 2008-03-12

Family

ID=39186214

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP070100213 AR059065A1 (en) 2006-01-23 2007-01-18 METHODS TO MODULATE THE MANOSA CONTENT OF THE RECOMBINANT PROTEINS

Country Status (3)

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CN (2) CN111676263A (en)
AR (1) AR059065A1 (en)
ZA (1) ZA200807126B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU231463B1 (en) * 2015-08-04 2024-01-28 Richter Gedeon Nyrt. Method for increasing the galactose content of recombinant proteins
CN116590371B (en) * 2023-07-13 2023-10-17 智享生物(苏州)有限公司 Cell culture method for reducing high mannose type antibody in Chinese hamster ovary cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925796A (en) * 1986-03-07 1990-05-15 Massachusetts Institute Of Technology Method for enhancing glycoprotein stability
WO2002101019A2 (en) * 2001-06-13 2002-12-19 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells
US7247304B2 (en) * 2001-08-23 2007-07-24 Genmab A/S Methods of treating using anti-IL-15 antibodies

Also Published As

Publication number Publication date
CN111676263A (en) 2020-09-18
ZA200807126B (en) 2009-07-29
CN101410528A (en) 2009-04-15

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