AR057542A1 - Procedimiento de seleccion implementada por ligandos en exceso. - Google Patents

Procedimiento de seleccion implementada por ligandos en exceso.

Info

Publication number
AR057542A1
AR057542A1 ARP060104421A ARP060104421A AR057542A1 AR 057542 A1 AR057542 A1 AR 057542A1 AR P060104421 A ARP060104421 A AR P060104421A AR P060104421 A ARP060104421 A AR P060104421A AR 057542 A1 AR057542 A1 AR 057542A1
Authority
AR
Argentina
Prior art keywords
ligands
selection procedure
procedure implemented
targets
selection
Prior art date
Application number
ARP060104421A
Other languages
English (en)
Original Assignee
Halitus Inst Medico S A
Radrizzani Helguera Martin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Halitus Inst Medico S A, Radrizzani Helguera Martin filed Critical Halitus Inst Medico S A
Priority to ARP060104421A priority Critical patent/AR057542A1/es
Priority to EP07118003A priority patent/EP1914321A3/en
Publication of AR057542A1 publication Critical patent/AR057542A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Procedimiento de seleccion implementada por ligandos en exceso que comprende un método estocástico basado en la probabilidad de existencia de un alto numeros de ligandos posibles contra un mismo blanco. El objetivo de este protocolo es el de proveer bibliotecas con concentraciones de ligandos compatibles a las concentraciones de equilibrio en solucion. La presente provee ligandos consistentes en oligonucleotidos y cada uno de ellos posee una estructura definida por su secuencia, capaz de reconocer el blanco utilizado para la seleccion. La ventaja de este método sobre los otros es la capacidad de obtener una gran cantidad de ligandos específicos en un solo paso y en unas pocas horas. Cuando se utilizan proteínas como blancos y bibliotecas combinatorias de oligonucleotidos, los ligandos obtenidos son de una afinidad del rango nanomolar y son sensibles a la conformacion de la misma. Además, este método permite reconocer blancos diferenciales de mezclas complejas, sin necesidad de sustancias puras.
ARP060104421A 2006-10-06 2006-10-06 Procedimiento de seleccion implementada por ligandos en exceso. AR057542A1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ARP060104421A AR057542A1 (es) 2006-10-06 2006-10-06 Procedimiento de seleccion implementada por ligandos en exceso.
EP07118003A EP1914321A3 (en) 2006-10-06 2007-10-06 Selection procedure by excess ligands

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP060104421A AR057542A1 (es) 2006-10-06 2006-10-06 Procedimiento de seleccion implementada por ligandos en exceso.

Publications (1)

Publication Number Publication Date
AR057542A1 true AR057542A1 (es) 2007-12-05

Family

ID=38829099

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP060104421A AR057542A1 (es) 2006-10-06 2006-10-06 Procedimiento de seleccion implementada por ligandos en exceso.

Country Status (2)

Country Link
EP (1) EP1914321A3 (es)
AR (1) AR057542A1 (es)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1078992A3 (en) * 1999-08-26 2003-08-27 Tomas Antonio Santa Coloma Polyclonal and Monoclonal Oligobodies (Synthetic, Oligonucleotide-Based Antibody-Like Reagents), and a method of producing oligobodies

Also Published As

Publication number Publication date
EP1914321A3 (en) 2009-03-04
EP1914321A9 (en) 2009-07-22
EP1914321A2 (en) 2008-04-23

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