AR037896A1 - PROCEDURE FOR THE TRANSFORMATION OF VEGETABLE PLASTIDES - Google Patents

PROCEDURE FOR THE TRANSFORMATION OF VEGETABLE PLASTIDES

Info

Publication number
AR037896A1
AR037896A1 ARP020104942A AR037896A1 AR 037896 A1 AR037896 A1 AR 037896A1 AR P020104942 A ARP020104942 A AR P020104942A AR 037896 A1 AR037896 A1 AR 037896A1
Authority
AR
Argentina
Prior art keywords
sequence
derived therefrom
recombination
dna
organism
Prior art date
Application number
Other languages
Spanish (es)
Original Assignee
Sungene Gmbh & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmbh & Co Kgaa filed Critical Sungene Gmbh & Co Kgaa
Publication of AR037896A1 publication Critical patent/AR037896A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Procedimiento para la integración específica de la secuencia de una secuencia de DNA en el DNA plastídico de un organismo vegetal o de células derivadas del mismo, en donde: a) las moléculas de DNA plastídicas de dicho organismo vegetal o de las células derivadas del mismo contienen por lo menos una secuencia de reconocimiento de recombinasa R1 y se reunen; b) i) por lo menos un constructor de transformación que comprende una secuencia de inserción flanqueada por lo menos unilateralmente por al menos otra secuencia de recombinación R2 así como también; ii) por lo menos una recombinasa específica de la secuencia adecuada para la inducción de eventos de recombinación en dichas secuencias de recombinación R1 y R2 en por lo menos un plástido de dicho organismo vegetal o de células derivadas del mismo; y c) se inserta la secuencia de inserción en el DNA plastídico bajo recombinación de R1 con R2; y d) se aíslan plantas o células, en las cuales se insertó la secuencia de inserción en la molécula de DNA plastídica. Cassette de expresión que contiene una secuencia de ácidos nucleicos que codifica para una recombinasa elegida entre el grupo de resolvasas/invertasas bajo control de un promotor activo en plástidos vegetales. Organismo vegetal que contiene el cassette de expresión anterior y cultivos celulares, órganos, tejidos, partes o elementos de reproducción transgénicos derivados del mismo. Uso del organismo o de cultivos celulares, órganos, tejidos, partes o elementos de reproducción transgénicos derivados del mismo como alimentos, forrajes o semillas o para la preparación de productos farmacéuticos o productos químicos puros.Procedure for the specific integration of the sequence of a DNA sequence in the plastidic DNA of a plant organism or of cells derived therefrom, where: a) the plastid DNA molecules of said plant organism or of the cells derived therefrom contain at least one R1 recombinase recognition sequence and meet; b) i) at least one transformation constructor comprising an insertion sequence flanked at least unilaterally by at least one other recombination sequence R2 as well as; ii) at least one sequence specific recombinase suitable for the induction of recombination events in said R1 and R2 recombination sequences in at least one plastid of said plant organism or of cells derived therefrom; and c) the insertion sequence is inserted into the plastidic DNA under recombination of R1 with R2; and d) plants or cells are isolated, in which the insertion sequence was inserted into the plastidic DNA molecule. Expression cassette containing a nucleic acid sequence encoding a recombinase chosen from the resolvase / invertase group under the control of an active promoter in plant plastids. Plant organism containing the previous expression cassette and cell cultures, organs, tissues, parts or transgenic reproductive elements derived therefrom. Use of the organism or cell cultures, organs, tissues, parts or transgenic reproductive elements derived from it as food, fodder or seeds or for the preparation of pharmaceutical products or pure chemicals.

ARP020104942 2001-12-20 2002-12-18 PROCEDURE FOR THE TRANSFORMATION OF VEGETABLE PLASTIDES AR037896A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10163159 2001-12-20

Publications (1)

Publication Number Publication Date
AR037896A1 true AR037896A1 (en) 2004-12-09

Family

ID=7710299

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP020104942 AR037896A1 (en) 2001-12-20 2002-12-18 PROCEDURE FOR THE TRANSFORMATION OF VEGETABLE PLASTIDES

Country Status (4)

Country Link
EP (1) EP1461439A1 (en)
AR (1) AR037896A1 (en)
AU (1) AU2002366865A1 (en)
WO (1) WO2003054201A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10236001A1 (en) * 2002-08-06 2004-02-19 Icon Genetics Ag Producing transgenic plants transformed in the plastome, by introducing two DNA segments each containing a region homologous with plastome nucleic acid
ATE542908T1 (en) 2002-10-15 2012-02-15 Syngenta Participations Ag PLASTIC TRANSFORMATION
AU2015202438B2 (en) * 2004-04-06 2017-05-25 Fibria Celulose S/A Cambium/xylem-preferred promoters and uses thereof
GB2449466B (en) * 2007-05-23 2009-11-18 Algentech Ltd Production of proteins in plant tissue
EP2199399A1 (en) 2008-12-17 2010-06-23 BASF Plant Science GmbH Production of ketocarotenoids in plants

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6632672B2 (en) * 1998-08-19 2003-10-14 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification
CA2379115C (en) * 1999-07-23 2011-04-26 The Regents Of The University Of California Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system
EP1222296A2 (en) * 1999-10-15 2002-07-17 Calgene LLC Methods and vectors for site-specific recombination in plant cell plastids

Also Published As

Publication number Publication date
AU2002366865A1 (en) 2003-07-09
EP1461439A1 (en) 2004-09-29
WO2003054201A1 (en) 2003-07-03

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Legal Events

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