AR022590A1 - METHOD FOR DETECTING SHIKIMIC ACID - Google Patents

METHOD FOR DETECTING SHIKIMIC ACID

Info

Publication number
AR022590A1
AR022590A1 ARP000100628A ARP000100628A AR022590A1 AR 022590 A1 AR022590 A1 AR 022590A1 AR P000100628 A ARP000100628 A AR P000100628A AR P000100628 A ARP000100628 A AR P000100628A AR 022590 A1 AR022590 A1 AR 022590A1
Authority
AR
Argentina
Prior art keywords
shikimic acid
chromophore
acid
detecting
tissue extracts
Prior art date
Application number
ARP000100628A
Other languages
Spanish (es)
Original Assignee
Syngenta Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Ltd filed Critical Syngenta Ltd
Publication of AR022590A1 publication Critical patent/AR022590A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Diabetes (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

La presente provee métodos para detectar ácido shikímico que comprenden los pasos de: oxidar ácido shikímico con ácido peryodico o un reactivo quecomprende peryodato y ácido peryodico; agregar una base fuerte para generar un cromoforo; estabilizaral cromoforo con sulfito; detectar la presencia delcromoforo; y optativamente cuantificar la cantidad del cromoforo. Los métodos de la presente pueden llevarse a cabo directamente con extractos de tejido,preferentemente extractos de tejido vegetal.This provides methods for detecting shikimic acid comprising the steps of: oxidizing shikimic acid with periodic acid or a reagent comprising periodate and periodic acid; add a strong base to generate a chromophore; stabilizaral chromoforo with sulphite; detect the presence of chromophore; and optionally quantify the amount of chromophore. The methods herein can be carried out directly with tissue extracts, preferably plant tissue extracts.

ARP000100628A 1999-02-19 2000-02-14 METHOD FOR DETECTING SHIKIMIC ACID AR022590A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US25328299A 1999-02-19 1999-02-19

Publications (1)

Publication Number Publication Date
AR022590A1 true AR022590A1 (en) 2002-09-04

Family

ID=22959622

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP000100628A AR022590A1 (en) 1999-02-19 2000-02-14 METHOD FOR DETECTING SHIKIMIC ACID

Country Status (11)

Country Link
JP (1) JP4410946B2 (en)
CN (1) CN1164943C (en)
AR (1) AR022590A1 (en)
AU (1) AU774136B2 (en)
BR (1) BR0008953B1 (en)
CA (1) CA2362977C (en)
IL (2) IL144946A0 (en)
MX (1) MXPA01008358A (en)
TW (1) TWI223067B (en)
WO (1) WO2000049399A1 (en)
ZA (1) ZA200106824B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102980866A (en) * 2012-11-26 2013-03-20 东北农业大学 Kit for testing in-vivo shikimic acid content of plants
CN107084943B (en) * 2017-04-28 2019-06-25 浙江大学 A kind of method of quick obtaining transgenic corns glyphosate tolerant phenotype
CN106896077B (en) * 2017-04-28 2019-05-28 浙江大学 The detection method of transgenic corns glyphosate tolerant phenotype based on imaging-PAM

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4294911A (en) * 1979-06-18 1981-10-13 Eastman Kodak Company Development of light-sensitive quinone diazide compositions using sulfite stabilizer
DK100592D0 (en) * 1992-08-10 1992-08-10 Mouritsen & Elsner Aps METHOD OF CHEMICAL CONNECTION ON SOLID PHASES

Also Published As

Publication number Publication date
AU2309800A (en) 2000-09-04
AU774136B2 (en) 2004-06-17
MXPA01008358A (en) 2003-06-06
BR0008953A (en) 2001-11-27
CN1351711A (en) 2002-05-29
BR0008953B1 (en) 2012-09-18
CN1164943C (en) 2004-09-01
CA2362977C (en) 2008-08-12
WO2000049399A1 (en) 2000-08-24
CA2362977A1 (en) 2000-08-24
ZA200106824B (en) 2003-02-17
IL144946A (en) 2007-03-08
IL144946A0 (en) 2002-06-30
JP4410946B2 (en) 2010-02-10
JP2002537559A (en) 2002-11-05
TWI223067B (en) 2004-11-01

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