AR021818A1 - PRODUCTION OF SOLUBLE RECOMBINANT TRIPSYNOGEN ANALOGS - Google Patents

PRODUCTION OF SOLUBLE RECOMBINANT TRIPSYNOGEN ANALOGS

Info

Publication number
AR021818A1
AR021818A1 ARP990104729A ARP990104729A AR021818A1 AR 021818 A1 AR021818 A1 AR 021818A1 AR P990104729 A ARP990104729 A AR P990104729A AR P990104729 A ARP990104729 A AR P990104729A AR 021818 A1 AR021818 A1 AR 021818A1
Authority
AR
Argentina
Prior art keywords
analogs
trypsinogen
trypsin
tripsynogen
production
Prior art date
Application number
ARP990104729A
Other languages
Spanish (es)
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of AR021818A1 publication Critical patent/AR021818A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invencion se refiere a los análogos de tripsinogeno y tripsina producidos en forma recombinante, incluyendo a los ácidos nucleicos quecodifican a los análogos de tripsinogeno y tripsina. Los análogos de tripsinogeno de la presente invencion contienen modificaciones de la secuencia líder detripsinogeno de forma tal que el tripsinogeno no puede ser clivado por las tripsina o enzimas tipo tripsina. La presente invencion también incluye los procesospara producir análogos de tripsinogenorecombinante que pueden ser subsecuentemente activado.The present invention relates to recombinantly produced trypsinogen and trypsin analogs, including nucleic acids that encode trypsinogen and trypsin analogs. The trypsinogen analogs of the present invention contain modifications of the detripsinogen leader sequence such that trypsinogen cannot be cleaved by trypsin or trypsin-like enzymes. The present invention also includes processes for producing trypsinogen-like analogs that can be subsequently activated.

ARP990104729A 1998-09-21 1999-09-20 PRODUCTION OF SOLUBLE RECOMBINANT TRIPSYNOGEN ANALOGS AR021818A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10121398P 1998-09-21 1998-09-21

Publications (1)

Publication Number Publication Date
AR021818A1 true AR021818A1 (en) 2002-08-07

Family

ID=22283537

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990104729A AR021818A1 (en) 1998-09-21 1999-09-20 PRODUCTION OF SOLUBLE RECOMBINANT TRIPSYNOGEN ANALOGS

Country Status (6)

Country Link
EP (1) EP1141263A4 (en)
AR (1) AR021818A1 (en)
AU (1) AU6388499A (en)
CA (1) CA2343966A1 (en)
PE (1) PE20001068A1 (en)
WO (1) WO2000017332A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7053500A (en) * 1999-09-15 2001-04-17 Eli Lilly And Company Chymotrypsin-free trypsin
US7276605B2 (en) 2001-02-01 2007-10-02 Roche Diagnostics Operations, Inc. Method for producing recombinant trypsin
DE60328802D1 (en) 2002-08-30 2009-09-24 Novozymes Inc PROCESS FOR THE PREPARATION OF MAMMALS TRYPSINS
CN104312933B (en) * 2014-10-17 2017-03-29 江南大学 A kind of method that optimization signal peptide improves the expression of trypsin exocytosiss
JP2018537976A (en) 2015-11-25 2018-12-27 アカデミシュ・ジークンホイス・ライデン Recombinant serine protease
CN116445462A (en) * 2023-04-20 2023-07-18 西安麦博泰克生物科技有限公司 Purification preparation method of recombinant porcine pepsin and recombinant porcine pepsin

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763215A (en) * 1984-08-16 1998-06-09 Bio-Technology General Corporation Method of removing N-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby
TW257792B (en) * 1992-10-01 1995-09-21 Lilly Co Eli
IL107536A0 (en) * 1992-11-13 1994-02-27 Lilly Co Eli Expression vectors for bovine trypsin and trypsinogen and host cells transformed therewith
US5773248A (en) * 1995-11-13 1998-06-30 Uab Research Foundation Nucleic acid encoding a human α3(IX) collagen protein and method of producing the protein recombinantly
JPH09294583A (en) * 1996-03-08 1997-11-18 Ajinomoto Co Inc Aminopeptidase gx and hydrolysis of protein using the same

Also Published As

Publication number Publication date
EP1141263A1 (en) 2001-10-10
EP1141263A4 (en) 2002-08-28
CA2343966A1 (en) 2000-03-30
PE20001068A1 (en) 2000-10-18
AU6388499A (en) 2000-04-10
WO2000017332A1 (en) 2000-03-30

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